...fluorescent protein that could be co-imaged with GFP using the same wavelength for excitation. The result...for how sensitive you can make a reaction. NLuc requires 100-fold less protein to produce the same amount...detecting traditional luciferases’ dim light emissions requires long acquisition times that may be impractical...

...are generated by inserting a circularly-permuted GFP into an intracellular loop of various G-protein-coupled...variations and heat transfer at the subcellular level requires a probe with fast kinetics and high signal/noise...

..., Hess et al. successfully evolved wild type GFP to EGFP using CRISPR-X and subsequent FACS sorting. They...with unique tissue-specific promoters expressing GFP, tetracycline response elements, and shRNAs many ... of DsRed connected to a pH-sensitive variant of GFP (SEP) by a 9 amino-acid linker (see Figure 1). The...for more selective targeting of fusion proteins, requires less biotin, and shows enhanced labeling of proximate...

...typically used for gRNA May contain reporter gene (e.g. GFP) to identify and enrich positive cells, or selection...transfer vectors May contain reporter gene (e.g. GFP) or selection marker to identify and enrich positive...targeting the region where the edit should be made HDR requires a repair template and displays reduced efficiency...

...labeling. Size is also a factor. Bulkier tags, such as GFP, may interfere with protein expression or function...another factor to consider. If your application requires longer periods of exposure, you’ll want a more...

...cassette comprised of OCT4, SOX2, LIN28, NANOG, and a GFP reporter. Minicircles are removed from cells by dilution...promoters for transcriptional regulation, so it requires a different approach for regulating transgene ...Generating iPSCs with plasmid-based expression requires serial transfection of 1 or 2 plasmids that express... the gold standard instead. Teratoma formation requires injecting iPSCs into immunodeficient mice and ...suited for these situations. The second scenario requires higher levels of safety and as little chance of...

...measurable through an epitope tag such as FLAG/His/GFP. Fluorescent protein tags, which correlate closely...antigen of interest in the given protocol. This requires use of one of the five pillars of antibody validation...

...typically used for gRNA May contain reporter gene (e.g. GFP) to identify and enrich positive cells, or selection...transfer vectors May contain reporter gene (e.g. GFP) or selection marker to identify and enrich positive...targeting the region where the edit should be made HDR requires a repair template and displays reduced efficiency...

...another marker, such as GFP. A researcher can use FACS to sort cells expressing GFP and later expand these...transcription of shRNAs. cDNA expression requires RNA pol II, and thus requires an RNA pol II promoter, such as...driven by the 5'LTR, which is a weak promoter and requires the presence of Tat to activate expression. The...

...contain a reporter gene (for example, luciferase or GFP) that offers a read-out of the activity of the genetic... regions of interest. In vitro DNA replication requires the 4 nucleotides, a DNA polymerase enzyme, the...tagged base is incorporated. Sanger sequencing requires a lot of DNA because the ultimate goal is to have...

...conditional allele, or floxed mouse, from Yang et al. requires two gRNAs targeting two intronic regions and two...allele The design for a floxed conditional allele requires you to target 2 loxP sites to intronic regions...commonly used cassettes, such as fluorescent proteins (EGFP, mCherry etc.), recombinases (Cre, Flp etc.), and...Overall, the main drawback of i-GONAD is that it requires a high-concentration, column-purified DNA template...

...original CAPTURE method The original CAPTURE method requires the creation of a stable cell line that co-expresses...expression. Additionally, CAPTURE 3C-based sequencing requires a large number of cells (~5x107). Together, these...lentiviruses delivering 1) a BioTAP-tagged dCas9 and an eGFP tag, and 2) gRNAs Number of loci targeted One ...

...fluorescent marker like green fluorescent protein (GFP), creating a customizable DNA or RNA label for fluorescence...indel formation compared to HDR. Prime editing requires more optimization and typically has lower efficiency...living cells. Visualizing a specific genomic locus requires recruitment of many copies of labeled proteins...gRNAs that tile the locus. Chromosome painting requires delivery of hundreds of gRNAs with target sites...

...and astrocytes located in the CNS, however, it requires high doses. Requests have shifted toward the more...relatively even distribution of target gene expression (EGFP, in the depositing manuscript) across the brain....

...fluorescent marker like green fluorescent protein (GFP), creating a customizable DNA or RNA label for fluorescence...indel formation compared to HDR. Prime editing requires more optimization and typically has lower efficiency...living cells. Visualizing a specific genomic locus requires recruitment of many copies of labeled proteins...gRNAs that tile the locus. Chromosome painting requires delivery of hundreds of gRNAs with target sites...