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We narrowed to 93 results for: IRES-GFP

Showing: 81 - 93 of 93 results

  1. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ...are generated by inserting a circularly-permuted GFP into an intracellular loop of various G-protein-coupled...variations and heat transfer at the subcellular level requires a probe with fast kinetics and high signal/noise...

  2. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ..., Hess et al. successfully evolved wild type GFP to EGFP using CRISPR-X and subsequent FACS sorting. They...with unique tissue-specific promoters expressing GFP, tetracycline response elements, and shRNAs many ... of DsRed connected to a pH-sensitive variant of GFP (SEP) by a 9 amino-acid linker (see Figure 1). The...for more selective targeting of fusion proteins, requires less biotin, and shows enhanced labeling of proximate...

  3. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...and labeled with primary antibody (0.01 µg/mL Anti-GFP [N86/38.1R] (Addgene #180084), 0.01 µg/mL Anti-HA...Addgene cloned EGFP into pAG Lenti CMV N-HA Puro (Addgene #236079) to create pAG Lenti CMV HA-EGFP Puro (Addgene...excited to share our very own set of empty, HA-, and EGFP-expressing lentiviral backbones designed right here... packaging and strong expression after inserting EGFP and provide step-by-step protocols for viral generation...Figure 2). Looking for more? Explore our empty and EGFP retroviral plasmid set, our bacterial plasmid set...where those interactions occur in the genome. This requires both targeting a specific genome location and ...

  5. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ...cassette comprised of OCT4, SOX2, LIN28, NANOG, and a GFP reporter. Minicircles are removed from cells by dilution...promoters for transcriptional regulation, so it requires a different approach for regulating transgene ...Generating iPSCs with plasmid-based expression requires serial transfection of 1 or 2 plasmids that express... the gold standard instead. Teratoma formation requires injecting iPSCs into immunodeficient mice and ...suited for these situations. The second scenario requires higher levels of safety and as little chance of...

  6. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...measurable through an epitope tag such as FLAG/His/GFP. Fluorescent protein tags, which correlate closely...antigen of interest in the given protocol. This requires use of one of the five pillars of antibody validation...

  7. Lentiviral Vector Guide

    Type
    Guide
    ...selectable marker. Fluorescent reporters, such as GFP, are another common selection marker. Instead of ... sorting (FACS) is used to sort cells expressing GFP and later expanded into a cell line. More information...as a promoter on the transfer plasmid, and this requires Tat for activation. Second-generation plasmids...vectors from your plasmids (Figure 5). This process requires a production cell line, typically human embryonic...

  8. Molecular Biology Reference

    Type
    Guide
    ...contain a reporter gene (for example, luciferase or GFP) that offers a read-out of the activity of the genetic... regions of interest. In vitro DNA replication requires the 4 nucleotides, a DNA polymerase enzyme, the...tagged base is incorporated. Sanger sequencing requires a lot of DNA because the ultimate goal is to have...

  9. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    ...conditional allele, or floxed mouse, from Yang et al. requires two gRNAs targeting two intronic regions and two...allele The design for a floxed conditional allele requires you to target 2 loxP sites to intronic regions...commonly used cassettes, such as fluorescent proteins (EGFP, mCherry etc.), recombinases (Cre, Flp etc.), and...Overall, the main drawback of i-GONAD is that it requires a high-concentration, column-purified DNA template...

  10. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

    Type
    Blog Post
    ...original CAPTURE method The original CAPTURE method requires the creation of a stable cell line that co-expresses...expression. Additionally, CAPTURE 3C-based sequencing requires a large number of cells (~5x107). Together, these...lentiviruses delivering 1) a BioTAP-tagged dCas9 and an eGFP tag, and 2) gRNAs Number of loci targeted One ...

  11. CRISPR Guide

    Type
    Collection
    ...fluorescent marker like green fluorescent protein (GFP), creating a customizable DNA or RNA label for fluorescence...indel formation compared to HDR. Prime editing requires more optimization and typically has lower efficiency...living cells. Visualizing a specific genomic locus requires recruitment of many copies of labeled proteins...gRNAs that tile the locus. Chromosome painting requires delivery of hundreds of gRNAs with target sites...

  12. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...and astrocytes located in the CNS, however, it requires high doses. Requests have shifted toward the more...relatively even distribution of target gene expression (EGFP, in the depositing manuscript) across the brain....

  13. CRISPR Guide

    Type
    Guide
    ...fluorescent marker like green fluorescent protein (GFP), creating a customizable DNA or RNA label for fluorescence...indel formation compared to HDR. Prime editing requires more optimization and typically has lower efficiency...living cells. Visualizing a specific genomic locus requires recruitment of many copies of labeled proteins...gRNAs that tile the locus. Chromosome painting requires delivery of hundreds of gRNAs with target sites...
Showing: 81 - 93 of 93 results