We narrowed to 771 results for: LIS
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TypeBlog Post...13226-x Cameron, P., Coons, M. M., Klompe, S. E., Lied, A. M., Smith, S. C., Vidal, B., Donohoue, P. D....
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Design Tips for Prime Editing
TypeBlog Post...., Levesque, S., Jing, Y., Simpson, D., Zhao, A., Li, H., Yan, W., Goudy, L., Schmidt, R., Solley, S. ... -
Hot Plasmids - November 2023
TypeBlog Post...in single cells. Find iPAK4 plasmids here! Lin D., Li X., et al. (2023). Time-tagged ticker tapes for intracellular...whole-brain reference atlas efforts, including a list of validated antibodies, on their webpage: mAb3D... -
Isolating Genomic Regions of Interest with the CRISPR System
TypeBlog Post...affinity purification with an anti-Cas9 antibody, the Li lab found a microRNA (miR483) that binds to the promoter... -
Split Fluorescent Proteins for Studying Protein-Protein Interactions
TypeBlog Post...interactions in living cells. Shao S, Zhang H, Zeng Y, Li Y, Sun C, Sun Y. Commun Biol. 2021 Yujie Sun ... -
Fluorescent Proteins 101: Fluorescent Protein Timers
TypeBlog Post...PubMed PMID: 19136976. 5. Miyatsuka, Takeshi, Zhongmei Li, and Michael S. German. "Chronology of islet differentiation... -
CRISPR Guide
TypeCollection...Bartolomei, M. S., Ferguson-Smith, A., Li, D., Yang, L., Wu, Y., & Li, J. (2015). CRISPR-Cas9-Mediated Genetic...: 25494202 Liu, X., Zhang, Y., Chen, Y., Li, M., Zhou, F., Li, K., Cao, H., Ni, M., Liu, Y., Gu, Z., Dickerson...: 23287718 Cui, Z., Tian, R., Huang, Z., Jin, Z., Li, L., Liu, J., Huang, Z., Xie, H., Liu, D., Mo, H...., J. A., Ran, F. A., Konermann, S., Agarwala, V., Li, Y., Fine, E. J., Wu, X., Shalem, O., Cradick, T....V., Nguyen, N. T., Zheng, Z., Gonzales, A. P. W., Li, Z., Peterson, R. T., Yeh, J. J., Aryee, M. J., &...29160308 Hess, G. T., Frésard, L., Han, K., Lee, C. H., Li, A., Cimprich, K. A., Montgomery, S. B., & Bassik...., Levesque, S., Jing, Y., Simpson, D., Zhao, A., Li, H., Yan, W., Goudy, L., Schmidt, R., Solley, S. ... -
Using AAV for Neuronal Tracing
TypeBlog Post...PubMed Central PMCID: PMC1462937. Dietzschold, B., Li, J., Faber, M., and Schnell, M. (2008). Concepts ...Sorensen, S.A., Slaughterbeck, C.R., Wakeman, W., Li, Y., Feng, D., Ho, A., Nicholas, E., Hirokawa, K....advantage of AAV vectors is that there are well-established protocols for their production and purification...AAV1, -2, -5, -7, -8 (Taymans et al., 2007), AAV1 (Hollis et al., 2008) AAV6 (Towne et al., 2010), AAV8, ...PMID: 19098898. PubMed Central PMCID: PMC2895694. Hollis, E. R. 2nd, Kadoya, K., Hirsch, M., Samulski, R... -
Antibodies 101: Normalization and Loading Controls for Western Blots
TypeBlog Post...saturate the detector. In between these extremes lies the linear detection range. Figure 4: The... -
Hot Plasmids: Fall 2024
TypeBlog Post...plasmids here! Zhong, B. L., Elliot, J. M., Wang, P., Li, H., Hall, R. N., Wang, B., Prakash, M., & Dunn, ...and in ternary complexes with other proteins, establishing CRBNmidi as a promising tool for future structure-guided... -
CRISPR 101: Homology Directed Repair
TypeBlog Post...Engineering Approaches with the CRISPR/Cas9 System. Li, K et al. PLoS ONE 9(8); (2014). PubMed. Making... -
Overcoming the AAV Size Limitation for CRISPR Delivery
TypeBlog Post...-4 Swiech L, Heidenreich M, Banerjee A, Habib N, Li Y, Trombetta J, Sur M, Zhang F (2014) In vivo interrogation... Originally published Jul 14, 2015 and last updated Sep 16, 2020 by Beth Kenkel. CRISPR genome editing...Cas12b and CasX. While there are no papers yet published that deliver these Cas proteins with AAV, at just... -
Antibodies 101: Reading a Flow Plot
TypeBlog Post...G., Chiu, A., Bader, A., Langan, E., Siebert, S., Li, J., Greiner, D., & Anderson, D. (2015). Neutrophil...strategy Figure 4 shows a gating strategy from a published experiment (Barlow-Anacker et al., 2017). This... -
Thoughts on the Future of Research 2015 Boston Symposium
TypeBlog Post...academic research environment. The “how many?” question lies at the base of all these questions as there is not... career development. Publishing Panel The Early Career Researchers in Publishing Panel brought in editors...Outcomes University websites do not currently publish career outcomes for PhDs or postdocs. Having this...Path Panel and the Early Career Researchers in Publishing panel, focused on showing researchers that they... PIs and all gave advice on how to succeed in publishing in the current academic environment. Some great...writing skills will help you in your academic publishing goals and make you a better communicator overall...have any questions or concerns throughout the publishing process. The diversity panel - Equity and inclusion... -
mTOR Pathway
TypeCollection...PMID: 22500797 . Rapamycin: one drug, many effects. Li J, Kim SG, Blenis J. Cell Metab. 2014 Mar 4;19(3)...Screening Kit Background mTOR Pathway Plasmids mTOR Gene List Resources The m echanistic or m ammalian t arget...available plasmids for the gene, or browse the gene list below. For multiple isoforms or subunits, individual...David Sabatini . Return to top mTOR Pathway - Gene List Click on a name to find available plasmids for the... for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail help... -
Antibodies 101: Introduction to Gating in Flow Cytometry
TypeBlog Post...luckily, the vast majority of my cells of interest lies within a clear negative population. When you’re ... -
Using Phosphoserine to Study Protein Phosphorylation
TypeBlog Post...PMID: 25514926. PubMed Central PMCID: PMC4383998. 3. Li, Tong, et al. "Cysteinyl‐tRNA formation: the last...difficulties with kinase specificity in vitro. The establishment of a system in which purified, recombinant protein...interested in the intersection of cell signaling, metabolism, and the mighty mitochondrion. You can follow... -
Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?
TypeBlog Post...common diseases. References Zhang Y, Yin C, Zhang T, Li F, Yang W, Kaminski R, Fagan PR, Putatunda R, Young...viral reservoirs, and CRISPR may be the way to accomplish this tough job! Kamel Khalili’s lab at Temple...infected cells, an idea that became much more realistic with the advent of CRISPR. Using CRISPR/Cas9 SAM...enough T-cells could be reached to ablate a well-established viral reservoir. A second challenge for CRISPR...developing resistance. Shortly after Kaminski et al. published their results, Wang et al. showed that HIV-1 could... -
Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods
TypeBlog Post...343-345. PubMed PMID: 19363495. 2. Wang JW, Wang A, Li K, Wang B, Jin S, Reiser M, Lockey RF. CRISPR/Cas9... -
Plant Plasmids and Resources
TypeCollection...editing across crop species and genotypes - Zhenghe Li. A robust CRISPR/Cas9 system for convenient, high-efficiency... for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail help...