...and fluorescent light sources. Visible light illuminates each cell as it passes through and scatters in...  Tips for flow cytometry success Break up cell clumps Success in a flow cytometry experiment depends ... through a nylon mesh cell strainer to break up clumps before running on the instrument. To sustain cell...

...injections are administered in the cisterna magna or lumbar spine. Image and caption courtesy of Challis et...parentheses in the"Mouse Strain-Specific Tropism?" column. For a complete list of mice strains with LY6A-...transducing the macaque brain, except for the cerebellum, brain stem, and spinal cord. All PALs, including...compared to AAV9 in the cerebrum, but not the cerebellum, in macaques. It also effectively transduces ...

...using an iodixanol column gradient. We'll show the loading and unloading of the column and give some tips...

...each plasmid needed for the whole experiment Total volume (μL) of plasmid needed for the whole experiment... the equation is labeled with its corresponding column in the calculator spreadsheet: We hope that this...

...Red2Green, a program that uses this waste as plastic lumber for things like truck bumpers, plastic adirondack...this material to produce various grades of plastic lumber and distribute it to industrial and residential...

... may be electrochemical or light-based, with luminescent and fluorescent reporters being especially popular...such as the nucleus, mitochondria, endoplasmic reticulum (ER), vacuole or cellular membranes. Examples ...

... require injection of substrate to produce bioluminescence. Substrate pharmacokinetics and biodistribution...localization: In vivo imaging with fluorescent or bioluminescent reporters usually only detects the signal of...

...encourage iGEM alumni to mentor younger students in society and to support iGEM alumni-industry networking...

... pyogenes Yeo pKS diaCas9_sgRNA 74923 Other/Phaeodactylum tricornutum U6 yes, cut S. pyogenes Winge pJJR50...

...a CRISPR array in the resulting Ignavibacteriae phylum genome. IgnaviCas9 is a hyperthermostable Cas9 ...IgnaviCas9 is most similar to a type II-C Cas9 from Parvibaculum lavamentivorans, whose optimal growth temperature...

...assay. Neutralization was quantified in terms of luminescence. Image from Garcia-Beltran et al., 2021 with...target cells and this can be detected via loss of luminescence.     B.1.351 variant neutralization escape ...

...species. Located at The Ohio State University in Columbus, Ohio, ABRC ships more than 100,000 samples to... for teachers looking to expand their science curriculum. Growing Arabidopsis in the classroom does not...

...iGABASnFR, and accumulates in the endoplasmic reticulum, but exhibits larger fluorescent responses to ...electrical activity through imaging. Since it’s illuminated by red/orange light, Archon1 can also be used...

... The amount of aeration, temperature, culture volume, antibiotic, and medium can all affect copy number...conditions aren't working against you!  The culture inoculum Freshly streaked bacteria have higher copy numbers...

... particles to your solution while still using a volume that’s compatible with your experimental setup.... the filter - When using a concentration filter column, make sure to rinse off the surface of the filter...

...new NEBNext Ultra II FS DNA Library Prep Kit for Illumina sequencing and took a class at NEB’s new teaching...prior NEBNext Ultra II DNA Library Prep Kit for Illumina, one was only able to extract libraries from previously...

...assemble hundreds of different plasmids from a single Illumina MiSeq® run. As shown in Fig. 2, below, differences...prepared as plexWell™ libraries, sequenced on an Illumina® sequencer, and then analyzed with our plasmid...

...calcium dynamics in the cytosol and sarcoplasmic reticulum are of interest to muscle biologists, while neuroscientists... Overview of AAV Encoded Calcium Biosensors Illuminating Epigenetics with a FRET Based Biosensor Fluorescent...

...Type Westerns and ELISAs mostly use HRP, a chemiluminescent conjugate that can be activated to emit light...stored either in a dark, opaque vial or wrapped in aluminum foil to prevent ambient light from degrading them...

...either through a chromogenic or chemiluminescent reaction. Chemiluminescence, which is light produced by ...