We narrowed to 149 results for: LYC

1. Antibodies 101: Buffers, Storage, and Conjugates

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   ...Antibodies 101: Monoclonal Antibodies Antibodies 101: Polyclonal Antibodies Antibodies 101: Introduction to Antibodies...

2. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

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   ...available in bacterial expression plasmids, glycoproteins and other proteins that require post-translational...

3. Antibodies 101: Conjugation

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   ...groups from cysteines, or carbohydrates from glycosylation (Dennler, et al., 2015). However, there are ...

4. Developing Lab Management Software for Biology

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   ...is a little simplified): Find the appropriate glycerol stock within our freezers Pick the sample into...

5. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

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   ...VSV-G and the baboon endogenous retrovirus Rless glycoprotein (BaEVRless) had high rates of transduction for...

6. Viral Vectors 101: Chemogenetics

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   ... with the IPD of an anion channel, such as the glycine receptor, will promote hyperpolarization. PSAM ...

7. Overcoming the Challenges of Lentiviral Production

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   ...sucrose gradient, spin columns, and polyethylene glycol (PEG) precipitation. All of these methods ultimately...

8. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

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   ... the plasmid that should be used to maintain a glycerol stock. Choose from: ampicillin, apramycin, blasticidin...

9. Illuminating Epigenetics with A FRET Based Biosensor

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   ...9 methylation, while the K27 reporter uses the polycomb chromodomain to recognise lysine 27 methylation...

10. Viral Vectors 101: Producing Your rAAV

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    ...your supernatant, you can simply use polyethylene glycol (PEG). We add 25 mL of 40% PEG solution to each...

11. 10 Basic tips for mammalian cell culture

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    ...Interfaces. Sana is particularly interested in Glycobiology and Immunology. You can find Sana on LinkedIn...

12. Plasmids 101: Multicistronic Vectors

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    ...downstream.4 The "cleavage" occurs between the Glycine and Proline residues found on the C-terminus meaning...

13. Tips for arabidopsis transformation

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    ...starter culture can be used for colony PCR and glycerol stocks. Dip/drip plants more than once: Dip your...

14. CRISPR 101: Ribonucleoprotein (RNP) Delivery

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    ...Two methods to accomplish this are Polyethylene Glycol (PEG) mediated transfection of protoplasts and ...

15. Plasmids 101: Colony PCR

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    ...standard for reference and to add a loading dye with glycerol to your samples before pipetting them onto the...

16. Your Lentiviral Plasmid FAQs Answered

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    ...generation of the transfer vector. Q5: What envelope glycoprotein will be expressed on virus particles? A5: This...

17. Antibodies 101: The Four ELISAs and When to Use Them

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    ... Selecting the Right Antibody Antibodies 101: Polyclonal Antibodies Resources on addgene.org The Addgene...

18. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

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    ... the cells could be batch-sorted to isolate a polyclonal cell line with strong gas vesicle expression ...

19. Twenty Years of Sharing: Expanding Accessibility

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    ...Transformation How to Design a Primer Creating Bacterial Glycerol Stocks Inoculating a Liquid Bacteria Culture ...

20. Viral Production at Addgene

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    ...preparation by precipitation in 10% polyethylene glycol (PEG) followed by centrifugation. Precipitated ...