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We narrowed to 148 results for: LYC

Showing: 81 - 100 of 148 results

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Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

Type

Blog Post

...available in bacterial expression plasmids, glycoproteins and other proteins that require post-translational...
Antibodies 101: Conjugation

Type

Blog Post

...groups from cysteines, or carbohydrates from glycosylation (Dennler, et al., 2015). However, there are ...
Developing Lab Management Software for Biology

Type

Blog Post

...is a little simplified): Find the appropriate glycerol stock within our freezers Pick the sample into...
Viral Vectors 101: Chemogenetics

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Blog Post

... with the IPD of an anion channel, such as the glycine receptor, will promote hyperpolarization. PSAM ...
Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

Type

Blog Post

... the plasmid that should be used to maintain a glycerol stock. Choose from: ampicillin, apramycin, blasticidin...
Overcoming the Challenges of Lentiviral Production

Type

Blog Post

...sucrose gradient, spin columns, and polyethylene glycol (PEG) precipitation. All of these methods ultimately...
Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

Type

Blog Post

...VSV-G and the baboon endogenous retrovirus Rless glycoprotein (BaEVRless) had high rates of transduction for...
Illuminating Epigenetics with A FRET Based Biosensor

Type

Blog Post

...9 methylation, while the K27 reporter uses the polycomb chromodomain to recognise lysine 27 methylation...
Viral Vectors 101: Producing Your rAAV

Type

Blog Post

...your supernatant, you can simply use polyethylene glycol (PEG). We add 25 mL of 40% PEG solution to each...
10 Basic tips for mammalian cell culture

Type

Blog Post

...Interfaces. Sana is particularly interested in Glycobiology and Immunology. You can find Sana on LinkedIn...
Plasmids 101: Multicistronic Vectors

Type

Blog Post

...downstream.4 The "cleavage" occurs between the Glycine and Proline residues found on the C-terminus meaning...
Tips for arabidopsis transformation

Type

Blog Post

...starter culture can be used for colony PCR and glycerol stocks. Dip/drip plants more than once: Dip your...
CRISPR 101: Ribonucleoprotein (RNP) Delivery

Type

Blog Post

...Two methods to accomplish this are Polyethylene Glycol (PEG) mediated transfection of protoplasts and ...
Plasmids 101: Colony PCR

Type

Blog Post

...standard for reference and to add a loading dye with glycerol to your samples before pipetting them onto the...
Your Lentiviral Plasmid FAQs Answered

Type

Blog Post

...generation of the transfer vector. Q5: What envelope glycoprotein will be expressed on virus particles? A5: This...
Twenty Years of Sharing: Expanding Accessibility

Type

Blog Post

...Transformation How to Design a Primer Creating Bacterial Glycerol Stocks Inoculating a Liquid Bacteria Culture ...
Antibodies 101: The Four ELISAs and When to Use Them

Type

Blog Post

... Selecting the Right Antibody Antibodies 101: Polyclonal Antibodies Resources on addgene.org The Addgene...
Viral Production at Addgene

Type

Blog Post

...preparation by precipitation in 10% polyethylene glycol (PEG) followed by centrifugation. Precipitated ...
New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

Type

Blog Post

... the cells could be batch-sorted to isolate a polyclonal cell line with strong gas vesicle expression ...
Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

Type

Blog Post

...genes involved in the production of a cell surface glycan that’s required for ricin uptake. The ricin screen...

Showing: 81 - 100 of 148 results

Results per page:

Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

Antibodies 101: Conjugation

Developing Lab Management Software for Biology

Viral Vectors 101: Chemogenetics

Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

Overcoming the Challenges of Lentiviral Production

Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

Illuminating Epigenetics with A FRET Based Biosensor

Viral Vectors 101: Producing Your rAAV

10 Basic tips for mammalian cell culture

Plasmids 101: Multicistronic Vectors

Tips for arabidopsis transformation

CRISPR 101: Ribonucleoprotein (RNP) Delivery

Plasmids 101: Colony PCR

Your Lentiviral Plasmid FAQs Answered

Twenty Years of Sharing: Expanding Accessibility

Antibodies 101: The Four ELISAs and When to Use Them

Viral Production at Addgene

New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

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