...reagents are appropriate for a given experiment. Make sure to check whether reagents are available to ...efficiency compared to NHEJ knockout. Base editors can make a limited set of mutations. Repress or Interfere...envelope plasmids provide the necessary components to make lentiviral particles (for details about lentivirus...

...Bacteria on an LB Agar Plate You may also like... Making LB Agar Plates Bacterial Transformation Recovering...the way you would hold a pencil, so that you can make a broad stroke. Only touch the surface of the plate... diagonal lines in another section of the plate. Make sure that the first line (and only the first) in...

... lentivirus be used to make stable cell lines? Lentiviruses can be used to make stable cell lines in the...plasmids be used in direct transfections as opposed to making virus? Some (but not all) lentiviral transfer plasmids...

...exhibited high levels of constitutive activity, making them less than ideal. DREADDs Designer Receptors... previously used in human populations in Japan, making it an attractive option for translational studies... workflows (such as laser or LED illumination), making LMOs a versatile option. Coupling brighter bioluminescent...

... invasive. Different activation wavelengths also make it possible to combine multiple opsins in the same...196: 1-28. PMID 22341318 Gradinaru V, Zhang F, Ramakrishnan C, Mattis J, Prakash R, Diester I, Goshen I,...PMID 20621963 Mattis J, Tye KM, Ferenczi EA, Ramakrishnan C, O'Shea DJ, Prakash R, Gunaydin LA, Hyun M...

... with a two-part Gibson reaction if you're only making a small change in a plasmid (such as point mutations...manufacturer's instructions. You can purchase master mix or make your own. Transform bacteria with the DNA and screen...overlapping PCR primers (>60 bp) but too short to make its own part (<150 bp). Pro-Tip Please note that...

...of a microscope are magnification (the ability to make an image larger) and resolution (the ability to ...through the sample. However, some of that light won’t make it through the sample because different components...fine focus knob (the smaller of the focus knobs) to make minor adjustments to the focus. After you are happy...

... of the rotor and place them in a stable rack. **Make sure not to disturb the gradient!** Collect Fractions...flow from the needle set at the 40–60% interface. Make sure that the microcentrifuge tubes are well positioned...spin, add more formulation buffer and sample and make sure to pipet back and forth a few times to mix ...

... mouse genes. Addgene is working with the TRC to make this shRNA cloning vector available to the scientific... microfuge tubes (do NOT use polystyrene tubes), make a cocktail for each transfection: 1 μg pLKO.1 shRNA...scientists with high-quality goods and services. Addgene makes every effort to ensure the accuracy of its literature...typographical or other errors may occur. Addgene makes no warranty of any kind regarding the contents of...

...plasmids. Animals - Antibodies produced in animals make use of the animal’s immune system. The antigen of...Overview of application Collect and lyse samples to make proteins available. Tissue samples may need additional...Ready to put your new knowledge to use? Addgene now makes ready-to-use recombinant antibodies made from plasmids...

...the risk of contaminating reagents we recommend making small aliquots of master mixes, primers, and probes...and place in the Biological Safety Cabinet (BSC). Make sure that the BSC to be used for dilution and the...wells. Cover the cartridge with the DG8 gasket, making sure that it is secure. Transfer the cartridge ...

...place it in the Biological Safety Cabinet (BSC). Make sure that the BSC is supplied with sufficient pipette...wells. Cover the cartridge with the DG8 gasket, making sure that it is secure. Transfer the cartridge ...the risk of contaminating reagents we recommend making small aliquots of master mixes, and primers/probe...

...repeated throughout a specific DNA sequence, which can make it difficult at times to identify compatible restriction...site-specific recombination or a ligation step, making it an easy, cheap and rapid cloning method. So ...

...Over-Agar Antibiotic Plating You may also like... Making LB Agar Plates Bacterial Transformation Recovering...transformed E. coli . Over-agar spreading of antibiotic makes it easy for an investigator to conveniently plate...

... Rule For each sample you want to load on a gel, make 10% more volume than needed because several microliters...For example, if you want to load 1.0 μg in 10μL, make 1.1μg in 11μL. Reference Page Top Index...

...sequencing primers, including: Is the primer unique? Make sure the primer only anneals once within the entire...incorrect sequence. Look at the peaks in the area and make sure they are justifiable peaks. For instance, ...

... that increase the transformation efficiency and make bacteria more susceptible to either chemical or ... plasmids at once. To save money, many labs also make their own competent cells. This is a relatively ...

...endonuclease negative E. coli strain such as NEB stable. Make a mixture of a total of 500 μL PEI-OptiPro SFM with...-Tip There can be batch to batch variation when making the PEI working stock, therefore the ratio of μg...

...plunger. Remove the entire pipette from the container making sure not to touch the sides of the container with...important for scientists to calibrate their pipettes to make sure that they are properly tuned to dispense the...

...solutions at various dilution. Make sure to use the polybrene-containing media to make the cell solution in this...