We narrowed to 527 results for: MEA;

...(usually a laser in fluorescence assays), which means luminescence assays have lower background levels...luciferase gene, and expression of luciferase is measured via the light produced. A similar approach can...assays require cell lysis as the most efficient means to disrupt the cell membrane and deliver the substrate...luciferase or alternative substrates can permit measurements of luminescence from live cells. Luciferase ...with experiments that supply both substrates and measure luciferase output at different wavelengths. Addgene...

...fixation and permeabilization protocol, but very poorly in a methanol fixation-permeabilization protocol.... can also be affected by cell fixation and permeabilization techniques — and so it may be necessary to...mixed in a 1:1 ratio and fixed in 4% PFA and permeabilized in 0.1% saponin. 400,000 cells were stained ...also save money!); and optimizing fixation/permeabilization reagents for intracellular targets. Since...sample with mixed expression, with the expression measurable through an epitope tag such as FLAG/His/GFP. ...

...between 0.80-1.20) would mean you have one copy of the gene; an RQ value of 2 means you have two copies, ...triplicate.  The nice thing about qPCR is that since it measures relative quantification - that is, since you’re... a light technical control - an RQ value of 1.5 means you have either made a pipetting error or an incredible...comparison of assays for accurate copy number measurement of the low-affinity Fc gamma receptor genes FCGR3A...

... antibodies may have similar emission spectra, meaning they will emit fluorescent light at similar wavelengths...record the fluorescence for both at similar levels, meaning that your populations will ‘look’ similar on your...two channels (Figure 1A). Applying compensation means you are telling your cytometer to adjust the fluorescent... does NOT change anything in your samples. This means that, unlike voltages, you can adjust compensation...compensate in the top right corner of a flow plot, meaning that it should be double-positive for the two markers...

...commonly used to measure relative quantification of mRNA, which can be used as a proxy measure for differences...primers. This separate thermocycle allows you to measure the intensity of signal over an entire cycle. Since...want to validate your primer/probe through another means, such as an agarose gel or uMelt analysis (Downey...verification. Primer dimers will often show up as a smear or band around 30-50 bp. uMelt analysis  uMelt is...previously, just because it’s used often doesn’t mean it’s right for your experiment! The model assumes...

...is the sum of the mean rate for each paper. Green and red lines depict the sum of mean +1 and -1 std for...Smoothing our plasmid order data with a 7-day rolling mean gave us a crude insight into the magnitude of the...of COVID-19 on data smoothed with a 7-day rolling mean. While this global approximation already shows... could be different.  Conclusion Figure 7: Mean inferred effects by region. In conclusion, our...

...single cells in a population. It can be used to measure protein expression, identify rare cells, or even... cytometers, and output is generated through measurement of events. An event is something that passes ...scatter (SSC) and forward scatter (FSC), which measure light that “scatters” past the cell, instead of... doublets and debris. The side scatter roughly measures granularity, a proxy for nucleic complexity of...it from left to right.  First, SSC and FSC are measured to differentiate cells from debris. Cells are ...

...and the distances the packages needed to travel meant the accessibility varied country to country.   Figure... you can imagine, shipping to so many countries means that our shipping team is always engaged in some...researchers all over the world — and we really do mean all over (Figure 3). We continue to add new posts...Stats and figures are helpful, but our favorite measure of our educational resources’ value is feedback...digital content. We have implemented proactive measures to find potential barriers that may not be obvious...

...inactivated native restriction/methylation system. This means the strain can neither restrict nor methylate DNA... proteins Tuner (DE3)   Contains mutated lac permease whch allows for linear control of expression ...most experiments, the lac promoter can “leak,” meaning that a low level of expression exists even without... these cases, some strains carry an additional measure of control such as the pLys plasmid, which suppresses...fear not—there are a few simple troubleshooting measures that can help get you back on track: Compatibility...

...of everyone in government (19). To defend the meaning of the word ‘fact’. Policy must be based on our...research, more funding is required. This is by no means an exhaustive list and every marcher is not necessarily...For science to be integrated into politics in meaningful ways, continued conversation about science’s ...on your state in this map. Finally, the march is meant to inspire scientists to be more involved in their...Better Science Nature: What Trump’s new travel ban means for science Nature: Meet the scientists affected...

...Addgene on #souptwitter or if you just enjoy a warm meal on a winter day, you should be happy to learn that...pSOUP to mCherry, satisfying ingredients reagents permeate the molecular biologist’s kitchen lab - and scientists...generated allows for qualitative and quantitative measurement of processes including RNA movement by tagging...hydroxybenzylidene imidazolinone (DFHBI). DFHBI is membrane permeable and non toxic to cells. Because it rapidly photobleaches...transcribed by RNA Polymerase III (5S, tRNA and U6) and measuring Corn fluorescence by flow cytometry, the Jaffrey...

... Zuckerberg Initiative, MedGen, ClinVar) and is meant to be representative but not exhaustive. If you ...

...about are (1) your experimental conditions and measurements; (2) the cells you’re targeting; (3) how much... et al., 2018; Korneyenkov & Zamyatnin, 2021), meaning what works great in one system or cell type can...need to test for this in your system if you’re measuring expression over a period of weeks (in vitro) or...cells you want to deliver to, and how you’ll be measuring your experimental output. You’ll also want to ..., at an adequate level for your experimental measurements. The number itself is determined through validation...

...high affinity for an endogenous molecular target, meaning the dye will be attached to the target through ...protein of interest, these tools provide an easier means to identify targets through fluorescent labeling...minimal background staining. Additionally, cell permeability is a critical factor, as it influences the dye's...light when excited. While it is technically cell-permeable, its use in live cells can sometimes result in...in cellular toxicity due to the permeation process. Advanced technologies in fluorescent dyes/ligands...

...familiar. The idea that my piecemeal degree might one day foster a meaningful day job only came into focus...interested in connecting with the public in a meaningful way, and not solely to ensure a profit. Often... of my thesis: a survey instrument designed to measure how individuals respond to issues of unsettled ...

...In the lab, it might mean persuasively articulating why the results you've measured should change established... another person.  In an interview setting, this means persuading an employer that your past work has prepared... their team.  When you're writing a grant, this means convincing a committee that your research proposal...

...antibody is a monoclonal antibody. But what does that mean, and how do monoclonal antibodies differ from other...antibody-producing B cells that cannot replicate, meaning that only hybridomas will survive this step. Then...then used to produce all lots of the antibody, meaning that the antibodies are largely identical between...antibody that is actually produced by the hybridoma, meaning that there will be changes between lots over time...

...organisms and cells they infect. In the lab, this means viruses can be engineered to be cell type specific...viruses that infect people in nature, extra safety measures, like replication incompetent viral particles,...are required. We will be covering these safety measures in a separate, upcoming post. If a viral vector...over adenovirus is their low immunogenicity, which means they do not stimulate a strong immune response when...

... attend. Make a date or two for beer, coffee or meals with colleagues you know will attend the conference...conference. Invite others to join you. A fun, memorable meal is a great way to build relationships. Make a list...leave an empty seat” here. If there are communal meals, sit with people you don’t know and put your phone...move on to science. More great tips on conference meal etiquette are here. Don’t only talk about science...

...processing, fixation, antigen-retrieval, and permeabilization will influence how the antibody interacts ... Selection Flow Chart    What does validated mean anyway? You now know that application validation...factor in antibody selection, but what does that mean? Not all validation is equal and you should think...proposed pillars.  Remember, validation does not mean that the antibody is guaranteed to work. Antibody-based...