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We narrowed to 526 results for: MEA;

Showing: 81 - 100 of 526 results

  1. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    ...is the sum of the mean rate for each paper. Green and red lines depict the sum of mean +1 and -1 std for...Smoothing our plasmid order data with a 7-day rolling mean gave us a crude insight into the magnitude of the...of COVID-19 on data smoothed with a 7-day rolling mean. While this global approximation already shows... could be different.  Conclusion Figure 7: Mean inferred effects by region. In conclusion, our...

  2. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ...and the distances the packages needed to travel meant the accessibility varied country to country.   Figure... you can imagine, shipping to so many countries means that our shipping team is always engaged in some...researchers all over the world — and we really do mean all over (Figure 3). We continue to add new posts...Stats and figures are helpful, but our favorite measure of our educational resources’ value is feedback...digital content. We have implemented proactive measures to find potential barriers that may not be obvious...

  3. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ...single cells in a population. It can be used to measure protein expression, identify rare cells, or even... cytometers, and output is generated through measurement of events. An event is something that passes ...scatter (SSC) and forward scatter (FSC), which measure light that “scatters” past the cell, instead of... doublets and debris. The side scatter roughly measures granularity, a proxy for nucleic complexity of...it from left to right.  First, SSC and FSC are measured to differentiate cells from debris. Cells are ...

  4. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    ...inactivated native restriction/methylation system. This means the strain can neither restrict nor methylate DNA... proteins Tuner (DE3)   Contains mutated lac permease whch allows for linear control of expression ...most experiments, the lac promoter can “leak,” meaning that a low level of expression exists even without... these cases, some strains carry an additional measure of control such as the pLys plasmid, which suppresses...fear not—there are a few simple troubleshooting measures that can help get you back on track: Compatibility...

  5. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ...Addgene on #souptwitter or if you just enjoy a warm meal on a winter day, you should be happy to learn that...pSOUP to mCherry, satisfying ingredients reagents permeate the molecular biologist’s kitchen lab - and scientists...generated allows for qualitative and quantitative measurement of processes including RNA movement by tagging...hydroxybenzylidene imidazolinone (DFHBI). DFHBI is membrane permeable and non toxic to cells. Because it rapidly photobleaches...transcribed by RNA Polymerase III (5S, tRNA and U6) and measuring Corn fluorescence by flow cytometry, the Jaffrey...

  6. March for Science

    Type
    Blog Post
    ...of everyone in government (19). To defend the meaning of the word ‘fact’. Policy must be based on our...research, more funding is required. This is by no means an exhaustive list and every marcher is not necessarily...For science to be integrated into politics in meaningful ways, continued conversation about science’s ...on your state in this map. Finally, the march is meant to inspire scientists to be more involved in their...Better Science Nature: What Trump’s new travel ban means for science Nature: Meet the scientists affected...

  7. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...about are (1) your experimental conditions and measurements; (2) the cells you’re targeting; (3) how much... et al., 2018; Korneyenkov & Zamyatnin, 2021), meaning what works great in one system or cell type can...need to test for this in your system if you’re measuring expression over a period of weeks (in vitro) or...cells you want to deliver to, and how you’ll be measuring your experimental output. You’ll also want to ..., at an adequate level for your experimental measurements. The number itself is determined through validation...

  8. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    ...high affinity for an endogenous molecular target, meaning the dye will be attached to the target through ...protein of interest, these tools provide an easier means to identify targets through fluorescent labeling...minimal background staining. Additionally, cell permeability is a critical factor, as it influences the dye's...light when excited. While it is technically cell-permeable, its use in live cells can sometimes result in...in cellular toxicity due to the permeation process. Advanced technologies in fluorescent dyes/ligands...

  9. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    ...familiar. The idea that my piecemeal degree might one day foster a meaningful day job only came into focus...interested in connecting with the public in a meaningful way, and not solely to ensure a profit. Often... of my thesis: a survey instrument designed to measure how individuals respond to issues of unsettled ...

  10. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    ...antibody is a monoclonal antibody. But what does that mean, and how do monoclonal antibodies differ from other...antibody-producing B cells that cannot replicate, meaning that only hybridomas will survive this step. Then...then used to produce all lots of the antibody, meaning that the antibodies are largely identical between...antibody that is actually produced by the hybridoma, meaning that there will be changes between lots over time...

  11. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    ...In the lab, it might mean persuasively articulating why the results you've measured should change established... another person.  In an interview setting, this means persuading an employer that your past work has prepared... their team.  When you're writing a grant, this means convincing a committee that your research proposal...

  12. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    ...organisms and cells they infect. In the lab, this means viruses can be engineered to be cell type specific...viruses that infect people in nature, extra safety measures, like replication incompetent viral particles,...are required. We will be covering these safety measures in a separate, upcoming post. If a viral vector...over adenovirus is their low immunogenicity, which means they do not stimulate a strong immune response when...

  13. How to Make Friends and Meet People at a Scientific Conference

    Type
    Blog Post
    ... attend. Make a date or two for beer, coffee or meals with colleagues you know will attend the conference...conference. Invite others to join you. A fun, memorable meal is a great way to build relationships. Make a list...leave an empty seat” here. If there are communal meals, sit with people you don’t know and put your phone...move on to science. More great tips on conference meal etiquette are here. Don’t only talk about science...

  14. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    ...processing, fixation, antigen-retrieval, and permeabilization will influence how the antibody interacts ... Selection Flow Chart    What does validated mean anyway? You now know that application validation...factor in antibody selection, but what does that mean? Not all validation is equal and you should think...proposed pillars.  Remember, validation does not mean that the antibody is guaranteed to work. Antibody-based...

  15. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    ...on the signaling molecule solely asmeans of visualization. This means that if all the primary antibodies...be time to look at the indirect approach - which means it’s time to talk about secondary antibodies. While...require their own incubation and wash steps, which means that indirect methods are often significantly longer...

  16. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

    Type
    Blog Post
    ...this operon can serve as a living biosensor for measuring caffeine content in samples. The Barrick lab and...concentrations in several beverages could be estimated by measuring growth of the ΔguaB pDCAF3 strain. Find the decaffeination...fLuctuation INcrease by Contact (FLINC)-based biosensors measure fluctuations of TagRFP-T fluorescence intensity...use to detect PKA activity can be generalized to measure phosphatase activity, second messenger activity...

  17. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ...mice, AAV is almost entirely non-immunogenic — meaning when conducting in vivo CRISPR screens, AAV is ...types. The combination of serotypes and psuedotypes means you can customize your CRISPR screen to a high degree...improve your selection pool for downstream NGS and means you can use smaller volumes of your library for ...while AAV only has approximately 4.7 kb — which means that when using Cas9, you’re already almost out ...

  18. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    ...However, the rate of transfer was variable. They measured transfer rates by comparing the number of colonies...isolated from cheese. Knockdown efficiency, as measured by knockdown of a constitutely expressed RFP reporter...median knockdown of ~40-fold across all species measured. Beyond understanding the basic biology of bacteria...frequency of each of the 40 library strains was measured after 6 and 12 generations with or without induction...

  19. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    ...electrical potential. Thankfully, there is a tool to measure these electrical events! Voltage indicators are...They can be targeted to the plasma membrane to measure membrane potential and can also be directed to ...vitro and a small molecule is added exogenously to measure a phenotypic response in the form of fluorescence...Indicators As previously mentioned, cellular voltage is measured at the cell membrane, and this voltage is particularly...

  20. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    ...facilitated the development of a simple and scalable means of making targeted genetic edits. Cas9 is a DNA .... Cas9’s ability to alternately serve both as a means of modifying DNA and of modulating gene expression...lines or transgenic animals expressing dCas9 as a means of modulating gene expression (6). Altering the ...sites of interest thus represents a straightforward means of exerting tight control over Cas9 nuclease activity...
Showing: 81 - 100 of 526 results