We narrowed to 261 results for: Mac
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TypeBlog Post... our purposes? In search of simple biological machines R bodies can repeatedly extend and contract...using R bodies: simple, force-generating protein machines that can extend and retract over many microns ...
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Antibodies 101: Buffers, Storage, and Conjugates
TypeBlog Post... which is then read by a detector panel in the machine. Fluorophores are light sensitive, so antibodies...idea to make sure that laser is available in your machine before you make your purchase. Picking a single... -
REPLACR Mutagenesis: Replacing In Vitro Recombination Methods
TypeBlog Post...recombineering, bacteria express phage recombination machinery, which facilitates easy genetic modification by...electrocompetent cells in which the phage recombination machinery is induced using L-arabinose. These cells can ... -
Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs
TypeBlog Post...instead use a preexisting, conserved cellular machinery to process gRNAs. Hijacking tRNA processing to...plants. Yang was intrigued by the tRNA processing machinery present in almost every living organism. Precursor... -
Supporting Rare Disease Research with the 2020 BeHEARD Awards
TypeBlog Post...that trigger the activation of dendritic cells, macrophages, T cells, and the recruitment of neutrophils....understand so that we could possibly develop pharmaceutical interventions that would inhibit IL-36 release... -
Rosella: A Fluorescent pH-Biosensor for Studying Autophagy
TypeBlog Post...unless autophagy is induced. Then non-selective macroautophagy of the cytosol leads to accumulation of Pho8Δ60.... E. (2015). Rapid parallel measurements of macroautophagy and mitophagy in mammalian cells using a single... -
Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation
TypeBlog Post... activity. They interact with transcriptional machinery to modify local gene expression, confounding any...genome and directly interacting with transcription machinery. Well, I’ve got some good news: complete... -
Synthetic promoter AAVs for cell-type specific expression in retinal cells
TypeBlog Post...were active in mouse retinas were injected into macaques and postmortem human retinas. About 25% of these... library for retinal cell expression in mouse, macaque, and man. Lab Anim 48, 264 (2019). https://doi.... -
Early Career Researcher Toolbox: Finding Relevant Papers
TypeBlog Post... in order to turn Twitter into a paper-finding-machine. There are four general types of accounts you can...Scholar analyzes publications and figures using machine learning to extract important features such as ... -
CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!
TypeBlog Post...Guided by dCas9, VP64 recruits transcriptional machinery to specific sequences, causing targeted gene regulation...of interest, this allows more transcriptional machinery to be recruited per targeted gene. Performance... -
BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development
TypeBlog Post...proteins assemble into functional multi-subunit macromolecular complexes, but a serendipitous discovery led... amino acid. Clearly, the development of a pharmacological intervention that rescues the function of even... -
Production of Virus in Insect Versus Mammalian Cells
TypeBlog Post...partially because they contain some of the necessary machinery to make these viruses. The cells are also easy...they require cloning specific AAV or lentiviral machinery into a baculovirus plasmid. Production of baculovirus... -
Hot Plasmids: Winter 2025
TypeBlog Post...inherited the genomic edit but not the editing machinery. Together, these advances have the potential to.... Example applications include large-scale pharmacological profiling or interrogation of context-dependent... -
Antibodies 101: Conventional vs Spectral Flow Cytometry
TypeBlog Post...to run larger panels: five laser spectral flow machines have 64 detectors, allowing for the use of up ...be distinguished using spectral flow since the machine uses the full spectral signature, not just a narrow... -
Chromoproteins: Colorful Proteins For Molecular Biology Experiments
TypeBlog Post...-colored protein from the jellyfish Cassiopea xamachana (Blanquet and Phelan, 1987). Eight years later...protein from the mangrove jellyfish Cassiopea xamachana. Marine Biology 94:423–430 . https://doi.org/10.1007... -
Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells
TypeBlog Post...for in vivo delivery of advanced genome editing machinery such as CRISPR-Cas. Figure 1: Schematic...transduced to target cells. Within target cells, Cas9 machinery is transiently expressed and binds to target DNA... -
Starter guide to induced pluripotent stem cells (iPSCs) part 1: A renaissance in regenerative medicine
TypeBlog Post...Kusumika (Kushi) Mukherjee, editor of Trends in Pharmacological Sciences, a Cell Press reviews journal. Stem...Kushi) Mukherjee is the Editor of Trends in Pharmacological Sciences, a Cell Press reviews journal. She... -
Plasmids 101: Protein Expression
TypeBlog Post...range of proteins including antibodies and pharmaceuticals, specifically interleukins. Yeast expression...components of the transcription and translation machinery. These include ribosomes, RNA polymerase, tRNAs... -
Addgene's Cancer Collection Pages Connect the Research Community
TypeBlog Post...behavior of cancer cells, attacking the cellular machinery necessary for division. This approach also kills...variety of cancers, Ras proteins have stumped the pharmaceutical industry for years. They have proven very difficult... -
Antibodies 101: Producing Recombinant Antibodies
TypeBlog Post...Edelhoch method. Int J Biol Macromol. 2015;77:260-5. doi: 10.1016/j.ijbiomac.2015.03.027. Epub 2015 Mar...