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Showing: 81 - 100 of 250 results

  1. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    ...recombineering, bacteria express phage recombination machinery, which facilitates easy genetic modification by...electrocompetent cells in which the phage recombination machinery is induced using L-arabinose. These cells can ...

  2. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ...instead use a preexisting, conserved cellular machinery to process gRNAs. Hijacking tRNA processing to...plants. Yang was intrigued by the tRNA processing machinery present in almost every living organism. Precursor...

  4. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    ...unless autophagy is induced. Then non-selective macroautophagy of the cytosol leads to accumulation of Pho8Δ60.... E. (2015). Rapid parallel measurements of macroautophagy and mitophagy in mammalian cells using a single...

  9. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    ... in order to turn Twitter into a paper-finding-machine. There are four general types of accounts you can...Scholar analyzes publications and figures using machine learning to extract important features such as ...

  10. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...inherited the genomic edit but not the editing machinery. Together, these advances have the potential to.... Example applications include large-scale pharmacological profiling or interrogation of context-dependent...

  11. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    ...partially because they contain some of the necessary machinery to make these viruses. The cells are also easy...they require cloning specific AAV or lentiviral machinery into a baculovirus plasmid. Production of baculovirus...

  12. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type
    Blog Post
    ...to run larger panels: five laser spectral flow machines have 64 detectors, allowing for the use of up ...be distinguished using spectral flow since the machine uses the full spectral signature, not just a narrow...

  13. Plasmids 101: Protein Expression

    Type
    Blog Post
    ...range of proteins including antibodies and pharmaceuticals, specifically interleukins. Yeast expression...components of the transcription and translation machinery. These include ribosomes, RNA polymerase, tRNAs...

  20. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    ...RNA that bind to a cell’s DNA, telling the Cas machinery where to cut.      Fig. 2: Heatmap indicating...): 250-252. Carlson J, Henn BM, Al-Hindi DR, Ramachandran S. Counter the weaponization of genetics research...
Showing: 81 - 100 of 250 results