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We narrowed to 154 results for: N-pac

Showing: 81 - 100 of 154 results

  1. Reflections and Looking Ahead

    Type
    Blog Post
    ...Virk, H., Laflamme, C., Houston, D. W., Polinski, N. K., Meijers, R., Levey, A. I., Saper, C. B., Errington...these milestones remind us of the extraordinary impact that happens when scientists come together to share...more AAV viral vector options, we launched AAV Packaged on Request, a service that dramatically expands... transfer plasmid in our repository can now be packaged on demand in five distinct serotypes, offering...breakthrough enabled brings us closer to making an impact on humanity's greatest challenges. Thank you for...

  2. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    ...10.1038/cr.2009.139 Janda, A., Bowen, A., Greenspan, N. S., & Casadevall, A. (2016). Ig Constant Region Effects...bind. We also know that changing the Fc domain can impact an antibody's binding affinity (Janda et al., 2016...

  3. An Addgene Summer Internship

    Type
    Blog Post
    ... Syntaxin3, and osa-miR528. My manager, Katherine N., reviewed the list and followed up with her manager...first week at Addgene, I had to get used to the workspaces, as well as complete the lab safety and cybersecurity...safety, general work skills — such as using Google workspace — and got the chance to work on my very own research...

  4. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    ... Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini LA, Zhang F. Science...blockbuster known as the CRISPR (clustered regularly interspaced palindromic repeats) Cas (CRISPR associated genes...genetic material next to the species-specific protospacer adjacent motif (PAM). Once the CRISPR/Cas complex...

  5. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ...Clevers H, Prasad V, Lebbink RJ, Rehmann H, Geijsen N. Cell. 2015 Apr 23;161(3):674-690. doi:10.1016/j.cell...to a protein of interest thereby giving it the capacity to enter cells. One such CPP was discovered in...directly with proteins removes the limitations of packaging size associated with viral vectors. For these ...

  6. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type
    Blog Post
    ..., Weyhenmeyer GA, O’Reilly CM, Lofton ME, Catalán N (2018) Ten simple rules for collaboratively writing...and novelty in scientific research and in any workspace. Re-design lab meetings with a focus on discussion...making lab meetings more interactive and creating space for discussion at the end of each talk - time for...

  7. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    ... four different categories.     Brookhouser, N., et al. BMC Biol 18, 193 (2020). https://doi.org/...several pooled libraries: Cas13X and Cas13Y, two compact Cas13 proteins found in the metagenome datasets...

  8. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ...highly specific genome editing. Tsai SQ, Wyvekens N, Khayter C, Foden JA, Thapar V, Reyon D, Goodwin MJ...Cas9 multiplexing in systems with limited packaging capacity. Still confused about the various tRNA expression... will be necessary to improve its multiplexing capacity. Multiplexing is key due to the natural redundancy...often necessary. Guide RNAs (gRNAs) are commonly packaged in 400-500 bp cassettes containing the RNA pol...100 bases) limit the number of gRNAs that can be packaged together in a single vector. In addition, the ...forms tRNA-gRNA and tRNA-gRNA-tRNA, with short 5’ spacer sequences preceding gRNAs, to test for gRNA cleavage...predicted with no addition of nucleotides to the 5’ spacers. The 3’ ends were modified slightly with either...

  9. Plan Your Experiment

    Type
    Collection
    ...Chen, J., Fulco, C. P., Jerby-Arnon, L., Marjanovic, N. D., Dionne, D., Burks, T., Raychowdhury, R., Adamson... T. M., Lander, E. S., Weissman, J. S., Friedman, N., & Regev, A. (2016). Perturb-Seq: Dissecting Molecular...Zukher, I., Dujardin, G., Sousa-Luís, R., & Proudfoot, N. J. (2023). Elongation roadblocks mediated by dCas9...vectors is that the packaging limit (how large of a DNA sequence you can actually package into your viral ...Edit References CRISPR ( C lustered R egularly I nterspaced S hort P alindromic R epeats) is a powerful system...efficiencies. The template sequences can also have a large impact on editing efficiency. So while testing multiple...promoters, and selection markers. There are also no packaging limits as there are for viral vectors — the only...

  10. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    ... Center at UNC Chapel Hill.   References Bennardo N, Gunn A, Cheng A, Hasty P, Stark JM (2009) Limiting...experiment. Here, we’ll discuss NHEJ, and how it impacts what happens to Cas9-mediated DSBs in the genome...active throughout the cell cycle and has a higher capacity for repair, as there is no requirement for a repair...chromosomes within a day. Another factor expected to impact repair is that the Cas9 protein doesn’t immediately...in determining how the structure of broken ends impacts how they are repaired. He works in Dale’s lab.  ...

  11. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ....The actual oligo you should order is:5'-CACCGN...N-3'3'-CN...NCAAA-5'You need to add the CACC and AAAC...Ran, F. A., Cox, D., Lin, S., Barretto, R., Habib, N., Hsu, P. D., Wu, X., Jiang, W., Marraffini, L. A...."CRISPR", short-hand for Clustered Regularly Interspaced Short Palindromic Repeats, in Pubmed returned...want to use double nickase, you could express two spacers and use PX335 to express the Cas9n (nickase). The...PAM sequence to the oligo when cloning my target spacers into the PX330 vector backbone? A2: There is no...usually adds it when they order the oligo. If your spacer sequence starts with a 'G', you naturally have ... compared to where the target site sequence (protospacer+PAM) is located? If there are other PAMs in the...

  12. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    ...Chipman P, Agbandje-McKenna M, Cleaver BD, Clément N, Byrne BJ, Zolotukhin S. A simplified purification...keep viruses frozen during shipment was having an impact on the samples. We therefore devised a few experiments...solution thawed, as the CO2 was confined to the tube headspace when the samples were frozen. This was clear by...tubes prior to thaw released the CO2 from the headspace, thus mitigating the pH effects of dry ice storage...

  14. Viral Vectors 101: Biosensors

    Type
    Blog Post
    ... you covered with packaging plasmids! References and Resources References  Bhalla N., Jolly P., et al...with some of our most popular biosensors already packaged and ready to deliver. Ready to give biosensors...

  16. Summer SciComm: Preprints

    Type
    Blog Post
    ... Open. https://doi.org/10.1242/bio.059310 Fraser N, Mayr P, Peters I (2021) Motivations, concerns and...scientists know the results, your work won’t have any impact! There are lots of ways scientists communicate...efforts, inspire future research, and increase their impact. Open access and transparency Another major benefit...

  17. Recombinases AAV Preps

    Type
    Collection
    ... Esteban Engel , Alexander Nectow 69570 pAAV-EF1a-N-CretrcintG EF1a none 1 Connie Cepko 69571 pAAV-EF1a-C-CreintG... Viral Vector Packaging Service AAV Recombinases Viral Vector Packaging Service: Recombinases ... Page . Don’t See What You’re Looking For? Our Packaged on Request service offers you even more options...Please note this does not guarantee viral vector packaging service, but lets us know what viruses would be...

  18. Deep Dive: qPCR

    Type
    Blog Post
    ....2008.112797. Epub 2009 Feb 26. PMID: 19246619.Downey N. Interpreting melt curves: An indicator, not a diagnosis...reaction set-up. Nucleic Acids Res. 31:e122.Thelwell N; Millington S; Solinas A; Booth J; Brown T. Mode of...multiplex your reference genes to save on plate space. For many cell lines and tissue types, appropriate...your reference gene set can save significant plate space and may be worth the initial effort of setting it...
Showing: 81 - 100 of 154 results