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We narrowed to 565 results for: PAC

Showing: 81 - 100 of 565 results

  1. Kit Free RNA Extraction

    Type
    Protocol
    ...hazards for these reagents. Work in a well-ventilated space and under a fume hood when working with the volatile...

  2. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    ... across regions, with Europe most impacted and China least impacted. The inferred weekend effect is, in...date range is, they could be the most impacted or least impacted. These results highlight the instability... last six months, COVID-19 has had a tremendous impact on our world -- as of June 4, 2020, COVID-19 has...unique PIs/labs placing orders per day) on dates impacted by each effect. The inferred parameters suggest...methods for fundamental scientific discovery and high-impact applications.       Alvin Hsu is a graduate student...next-generation therapeutics. Prime editing, base editing, PACE, and DNA-templated synthesis are four examples of...

  3. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...ATAACTTCGTATAgcatacatTATACGAAGTTAT 13 bp inverted repeats + 8 bp spacer (34 bp) lox2272, lox5171, lox511, loxN, lox66/lox71...TAACTTTAAATAATgccaATTATTTAAAGTTA 14 bp inverted repeats + 4 bp spacer (32 bp) Currently, only the canonical rox site ...GAAGTTCCTATTCCGAAGTTCCTATTCtctagaaaGTATAGGAACTTC 13 bp inverted repeats + 8 bp spacer (48 bp or 34 bp) F3, F5, FRT14, FRT15 minimal FRT...feature Cre-dependent tools, or consider our AAV Packaged on Request service for more options. Addgene blog...feature Flp-dependent tools, or consider our AAV Packaged on Request service for more options. Addgene blog...

  4. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

    Type
    Blog Post
    ...amount of spurious DNA from the packaging cell line, helper and packaging plasmids. We decided to use this... genome exists as [+] and [-] strands that are packaged at the same frequency, we reasoned that, following...viral genome sequencing to determine serotype AAV packaging is not perfect and while we think of the AAV vector...not require any additional reagents or lab work (Pacouret, 2017). Moreover, the sequencing data from multiple...Method for Rapid and Inexpensive Validation of Packaged Single-Stranded Adeno-Associated Viral Genomes...195–205 . https://doi.org/10.1089/hgtb.2019.110 Pacouret S, Bouzelha M, Shelke R, Andres-Mateos E, Xiao...

  5. Optogenetics AAV Preps

    Type
    Collection
    ... Viral Vector Packaging Service AAV Optogenetics Viral Vector Packaging Service: Optogenetics ...Promoter Opsin Variant Tag Activity Serotype PI 20071 pACAGW-ChR2-Venus-AAV CAG ChR2 Venus Constitutive 1, 9... Page . Don’t See What You’re Looking For? Our Packaged on Request service offers you even more options...Please note this does not guarantee viral vector packaging service, but lets us know what viruses would be...

  6. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ..., ABE8s resulted in 1.5-fold more editing at protospacer positions A5-A7 and 3.2-fold more editing at ...to ABE7.10. ABE8s have an improved base editing capacity, even at sites previously difficult to target....Joung’s lab created a dual-deaminase editor called SPACE (synchronous programmable adenine and cytosine editor...and adenine base editors Grünewald et al., 2020 SPACE   Fused cytosine and adenine base editors Walton...01532-7 Gaudelli, N. M., Komor, A. C., Rees, H. A., Packer, M. S., Badran, A. H., Bryson, D. I., & Liu, D.... J. M., Lee, S.-J., Liquori, A. J., Murray, R., Packer, M. S., Rinaldi, C., Slaymaker, I. M., Yen, J.,...020-0535-y Kim, Y. B., Komor, A. C., Levy, J. M., Packer, M. S., Zhao, K. T., & Liu, D. R. (2017). Increasing...

  7. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    ...expression at varying capacities. AAV vectors containing the AAV2 ITRs can be cross-packaged with capsids from...replication and are required for replication, packaging, and vector persistence. They work in conjunction...in the same DNA molecule as the gene that they package. In a transfer plasmid, the transgene is flanked...deleting the B-B' or C-C' arms can reduce the packaging efficiency but increase transgene expression (...Terminal Repeats (ITRs) on the Productivity, Genome Packaging, and Potency of AAV Vectors. Microorganisms, 12...Bonnin, D., Merten, O.-W., & Galibert, L. (2017). Impact of Inverted Terminal Repeat Integrity on rAAV8 ...

  8. Important Considerations When Using AAVs

    Type
    Blog Post
    ...Richard J. Samulski. "Packaging capacity of adeno-associated virus serotypes: impact of larger genomes on...cells of interest. Although they have a small packaging capacity, AAV vectors were suitable for our needs ...recombinant adeno-associated viral vectors: packaging capacity and the role of rep proteins in vector purity...bleach solution should be used to clean AAVs from workspaces. Speed: AAV transduction experiments are ...

  9. Zinc Finger Consortium: Nuclease Expression Vectors

    Type
    Collection
    ...for expression of a ZFN targeted to a site with a spacer of 7 bps and harboring a “+” heterodimeric FokI...for expression of a ZFN targeted to a site with a spacer of 7 bps and harboring a “-” heterodimeric FokI...expression of a ZFN targeted to a site with a 5 or 6 bp spacer and “+” KKR heterodimeric FokI domain. ( Doyon ...expression of a ZFN targeted to a site with a 5 or 6 bp spacer and “-” ELD heterodimeric FokI domain. ( Doyon ...

  10. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ...AAV components      Cargo capacity Empty rAAV has a cargo capacity of ~ 4.7 kb, meaning that your... is a single stranded, Parvoviridae DNA virus, packaged in a non-enveloped icosahedral capsid, that can...in recombinant AAVs (rAAVs) with maximum “trunk” space for genetic cargo. Typically, when researchers speak...the type of capsid (outer protein shell) used to package an rAAV vector; these capsids allow the virus to...design single rAAV vectors with multiple genes packaged in them and use elements such as IRES or 2A to...rescued from the vector plasmid and individually packaged into the AAV capsids. As a genome conversion in...

  11. Caltech Systemic Capsids

    Type
    Collection
    ... Viral Vector Packaging Service AAV Caltech Systemic Capsids Viral Vector Packaging Service: Caltech...CAP-B22 AAV9-X1.1 As part of our Viral Vector Packaging Service , Addgene is distributing ready-to-use...window) Don’t See What You’re Looking For? Our Packaged on Request service offers you even more options...Please note this does not guarantee viral vector packaging service, but lets us know what viruses would be...

  12. Hot Plasmids: Summer 2025

    Type
    Blog Post
    ...for cell barcoding Addgene’s own AAV6 and AAV11 packaging plasmids Mapping anterograde synaptic connections...especially among plant biologists, due to their compact size and ability to generate heritable edits (learn...These libraries will be useful for studying the impacts of other cancer mutations and non-genetic diversity...10.1016/j.stem.2025.01.012.  New AAV6 and AAV11 packaging plasmids from Addgene By Meghan Rego Attention...your gene delivery toolkit, Addgene now offers packaging plasmids for AAV6 and AAV11! Why these serotypes...? Browse our In-Stock AAV inventory or use our Packaged on Request service for thousands of eligible AAV...use in your lab. Find Addgene’s AAV6 and AAV11 packaging plasmids here!   An ATLAS for anterograde synaptic...

  14. AAV Production in HEK293 Cells

    Type
    Protocol
    ...degrade any residual DNA carried over from the packaging process. Incubate at 37 °C for 45 min. You are...

  15. Serotype Testing AAV

    Type
    Collection
    ... Viral Vector Packaging Service AAV Serotype Testing AAV Viral Vector Packaging Service: Serotype ...order it? Don’t See What You’re Looking For? Our Packaged on Request service offers you even more options...Please note this does not guarantee viral vector packaging service, but lets us know what viruses would be...

  16. #DeckTheLab with Tinsel and Test Tubes, Fa La La La La La La La La

    Type
    Blog Post
    ...simply embellish and adorn your lab space or your work from home space in a fun, festive holiday or winter...selected as a winner, you’ll receive a winter care package from Addgene.  Here are the details:   Contest...winter or holiday theme decor in your work from home space… but with a science twist. Maybe you have a collection...using life science catalog pages. Make your WFH space feel a little more festive this holiday season! ...Instagram as we share pictures of our decked out spaces.       ...

  17. E11 Bio PRISM Collection

    Type
    Collection
    ...blood-brain barrier such as PHP.eB. The plasmids can be packaged as AAVs and pooled together for co-delivery. Alternatively...plasmids can be combined at equimolar ratios and packaged together in one AAV preparation. The depositing...production runs small to limit the amount of bias in packaging representation. To read out the barcodes, each...in a new window) More Resources at Addgene: AAV Packaged on Request AAV Guide Cre-Lox Plasmid Collection...

  18. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    ...based on the location of the ‘A’ relative to the protospacer adjacent motif (PAM). ABE7.10 can edit target...'A' if it lies in a small genomic window: at protospacer position 4-8 counting the ‘NGG’ PAM as 21-23 ...closer to the PAM, i.e., positioned at 8-10 in protospacer, ABE6.3 or ABE7.9 are better suited (Gaudelli...two Bsa1 sites to clone the single guide RNA (protospacer+scaffold) in a pol-III (OsU3) promoter driven...tRNA-gRNA (PTG) gene system to clone multiple protospacers which enables us to target more than one genomic...placed in 2-6 bp and 4-8 bp, respectively, of the protospacer. And if the target cytosine is closer to the ...depending on the location of your target base in the protospacer sequence. If you do not find a suitable ‘NGG’...

  19. Recombinases AAV Preps

    Type
    Collection
    ... Viral Vector Packaging Service AAV Recombinases Viral Vector Packaging Service: Recombinases ... Page . Don’t See What You’re Looking For? Our Packaged on Request service offers you even more options...Please note this does not guarantee viral vector packaging service, but lets us know what viruses would be...

  20. AAV for Neuronal Tracing

    Type
    Collection
    ... Viral Vector Packaging Service AAV Monosynaptic Neuronal Tracing Viral Vector Packaging Service: Tools...17329205 Don’t See What You’re Looking For? Our Packaged on Request service offers you even more options...Please note this does not guarantee viral vector packaging service, but lets us know what viruses would be...
Showing: 81 - 100 of 565 results