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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 1,014 results for: PLE

Showing: 81 - 100 of 1014 results

  1. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type
    Blog Post
    ... have no fear - I’m here to introduce you to a simpler, yet more effective, CRISPR-based epigenetic approach...machinery.   Well, I’ve got some good news: complete DNA demethylation can be achieved by dCas9 alone...Sapozhnikov and Szyf 2021).    Figure 3: A simpler approach - dCas9 blocks DNMT1 methylation, leaving...too.  High dCas9 and gRNA expression are key for complete DNA demethylation – tight binding is crucial for...blasticidin or puromycin resistance genes.  It’s that simple! Good luck and happy editing!    Thank you to...

  2. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

    Type
    Blog Post
    ... one simple experiment. PCA is an in vivo technique where two proteins are fused to complementary reporter...neighboring proteins. Thus to capture protein complexes, scientists can simply express a bait protein ...suboptimal folding or stability. Thus they conducted multiple rounds of directed evolution until they identified...interactions Split-BioID combines a protein fragment complementation (PCA) technique with proximity labelling to...labeling and purifying additional proteins from a complex of interest. For more information, read the Split-BioID...detect RNA-protein interactions that can regulate multiple biological functions. RapID (RNA-protein interaction... and Paul A. Khavari. "Research Techniques Made Simple: Emerging Methods to Elucidate Protein Interactions...

  3. Michael Koeris' Journey from Grad Student to Entrepreneur: The Story of Sample 6

    Type
    Blog Post
    ...Novophage to Sample 6. To learn more about Novophage's successful transformation into Sample 6 and Dr. ... in food products prior to Sample 6 16:27 - 18:02 : Benefits of the Sample 6 technology and how It works...- 19:56 : Why Sample 6's first test was for Lysteria 19:56 - 24:41 : Who is using Sample 6 and why? 24...Resources Visit the Sample 6 website Check out Dr. Koeris' Website, biotechstart.org Read Sample 6's Paper on...for use in a biotech startup 12:16 - 15:15 : Why Sample 6 began to develop tests for food products as opposed...Directors. We're excited to watch both Addgene and Sample 6 grow with his help! Special thanks to our sound... sample6...

  4. Chemogenetics AAV Preps

    Type
    Collection
    ...available items found to match all requested criteria. Please consider using fewer filtering options. *AAVrg ...would like to see available in viral prep format. Please note this does not guarantee viral vector packaging...

  5. Worm Expression Resources

    Type
    Collection
    ...below and not included in this table. For a more complete list of plasmids that can be used in worms, search...backbones, and constructs for endogenous tagging. Some examples of useful worm CRISPR resources, along with links...

  6. AAV Packaged on Request

    Type
    Collection
    ...transfer plasmid, including legal approval and an implementation letter DNA amplification Viral vector production...researchers with troubleshooting and results that complement published data, capturing the insights that often...

  7. p53 Pathway

    Type
    Collection
    ...for the gene, or browse the gene list below. For multiple isoforms or subunits, individual links to each...available plasmids for the gene. For components with multiple isoforms or subunits, individual links to each...

  8. Introducing an All-in-One CRISPR/Cas9 Vector System for Multiplex Genome Engineering

    Type
    Blog Post
    ...construction system for multiplex genome engineering. Image from Sakuma at al. (2015). Multiplex genome engineering... construction system for CRISPR/Cas9-mediated multiplex genome engineering is now available thanks to ...new tool in Scientific Reports in June. “The multiplexity is one of the most advantageous properties of...established way of making an all-in-one vector for multiplex genome engineering.” Now, they’ve filled that...Cas9-nickase vectors work successfully to target multiple sites in cultured human cells. While it has not...be applied to efficient viral vector-mediated multiplex genome engineering. The system now provides an...an efficient targeting strategy for multiplex editing of genomes. It will also allow simultaneous activation...

  9. Control AAV Preps

    Type
    Collection
    ...available items found to match all requested criteria. Please consider using fewer filtering options. Browse ...would like to see available in viral prep format. Please note this does not guarantee viral vector packaging...

  10. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

    Type
    Blog Post
    ... to implement in practice because FRET depends on a number of hard-to-achieve factors. For example, FRET...Bimolecular Fluorescence Complementation (BiFC). What is bimolecular fluorescence complementation? Bimolecular fluorescence... TK (2006) Design and implementation of bimolecular fluorescence complementation (BiFC) assays for the...often easier said than done. Fortunately, a complementary method exists for visualizing protein-protein... fluorescence complementation is a method for detecting protein-protein interactions that uses split fluorescent...fragments that individually lack fluorescence. When complementary FP fragments are fused to two hypothesized protein...interaction partners (PIPs), the FP fragments form the complete FP because the PIPs they’re tethered to bring ...

  11. TALENs for Endogenous Zebrafish Genes

    Type
    Collection
    ...TGGATGGCGAGAACAGGGcggtggagtataacgcTGTGGGCCTGACGAAGGA Complement Factor H TAL3230 & TAL3231 TGATTACATGTGAACCTCcatcacgtgaccagcatgTGTCCACCTCCTTTTGGA...TTTCCTCTCATAGTCAATattaattctctatttgGCTCAAAATGTCAGTAAA Plekho1b-1 TAL3142 & TAL3143 TCGTCAAAGCGGGGCCCTcaggatgccaatcaacAGCCTGTGCAGCCCGACA...

  12. Optogenetics AAV Preps

    Type
    Collection
    ...available items found to match all requested criteria. Please consider using fewer filtering options. Browse ...would like to see available in viral prep format. Please note this does not guarantee viral vector packaging...

  13. Retrograde AAV viral preps

    Type
    Collection
    ...available items found to match all requested criteria. Please consider using fewer filtering options. Browse ...would like to see available in viral prep format. Please note this does not guarantee viral vector packaging...

  14. Plasmids for Stem Cell Research

    Type
    Collection
    ...or somatic) stem cells, can differentiate into multiple cell types, but are typically limited to related...generate iPSCs. The generation of iPSCs is relatively simple in concept: ectopically express a cocktail of stem...

  15. Biosensor AAV Preps

    Type
    Collection
    ...available items found to match all requested criteria. Please consider using fewer filtering options. *AAVrg ...would like to see available in viral prep format. Please note this does not guarantee viral vector packaging...

  17. Synthetic Biology - Metabolism

    Type
    Collection
    ... related to metabolic pathways and components. Examples Include: Biofuels Natural Product Pathways Efflux...

  20. MAPK Plasmids

    Type
    Collection
    ...programs by acting as an integration point for multiple biochemical signals. In collaboration with numerous...
Showing: 81 - 100 of 1014 results