We narrowed to 255 results for: PORA

...delivered to target cells using microinjection or electroporation. Transient expression of CRISPR components,...Easy Easy Easy Physical transfection   Electroporation / Nucleofection Microinjection Cells Many...

... Protein tags are usually smallish peptides incorporated into a translated protein. As depicted in the... are available, but scientists would need to incorporate their recognition sites into their protein tag...

...tumefaciens, a plant-infectious bacteria that can incorporate foreign DNA into plant genomes. Eukaryotes are...stable transfection, DNA from the plasmid is incorporated into the host cell’s genome, so no special sequence...

...synthesis polymerase known to preferentially incorporate A bases across from abasic sites. This approach...a template — in this case, encouraging it to incorporate a new base across from the abasic site before...

...Find the plasmids at Addgene! CRISPR RNP electroporation and AAV donor infection (CRISPR-READI) enables...GRABNE) sensors allow for sensitive and high spatiotemporal measurements of norepinephrine. These norepinephrine...

...into cells by electroporation. hiPSC colonies are obtained by 10 days after electroporation, most of which...

...either fold to incorporate their target molecule, such as a metal ion, or fold to incorporate themselves ...

...2024). They developed a new prime editor that incorporates La’s N-terminal domain, PE7 (Figure 2).  PE7...high microglia-specific gene expression when incorporated in an AAV vector (Figure 5). Efficiency and ...

...Xanthomonas campestris   In addition to incorporating broad host range replicons, recombinant vectors...can be expressed. For example, scientists can incorporate interchangeable antibiotic resistance cassettes...

...ICSS can now be done with neuronal cell-type and temporal precision. In ICSS, a test chamber is used in ...more precisely characterized with spatial (CA1), temporal (real-time inhibition), and behavioral (remote...

...version of my manual, I added new chapters to incorporate the experiences of the people that provided me...interviews. We didn't have Skype in 2004. I also incorporated example documents. This part has generated a...

... scientists to easily control the level and spatiotemporal expression of a gene of interest. This type...method that uses heat induction to control the temporal expression of different transgenes, the Stainer...

... of exogenous biotin allowing for more precise temporal control.  Find plasmids for TurboID and miniturbo...proteomics approach to monitor the composition of spatiotemporally defined protein complexes." Nature communications...

...lengths because extension is terminated upon the incorporation of a ddNTP. Researchers perform four different...activity as fluorescently labeled nucleotides are incorporated, a method called pyrosequencing. The drive for...

...one of the most important ways to spatially and temporally control gene expression, especially in in vivo... 8 colors. The use of AAV provides spatial and temporal control without the need for germline modification...

...individual types of GPCRs with greater spatial and temporal control than is achieved by traditional pharmacologic...unknown.      Figure 3: Chimeric OptoXR incorporating rhodopsin undergoes a conformational change ...

...obstacle, a positive selection cassette can be incorporated, and used during the differentiation process...homologous recombination, which results in the incorporation of your specific edit into the host genome. ...

...Ridley Scott wonder what would happen if these corporations would extrapolate genetic engineering to exploit...which are genetically engineered by a powerful corporation to serve as slaves.  Meganucleases In 1989 the...

...system to modify your target DNA, you first electroporate a linear donor DNA substrate (either dsDNA or...Expression of lambda red recombination genes. Electroporation of substrate DNA and outgrowth of bacteria....

...amino acids to directly incorporate a fluorophore in your protein or to incorporate an alkene to be labeled...