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We narrowed to 257 results for: PORA

Showing: 81 - 100 of 257 results

  1. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...upon these original tools that allows for both spatiotemporal protein degradation and quantification of this...expressed under a desired promoter with a specific spatiotemporal expression pattern and an auxin inducible degron...

  2. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ...interest. However, FACS is unable to spatially and temporally target single cells. Now, Francois St-Pierre’...way to isolate individual cells with unique spatiotemporal phenotypes from large heterogeneous cultures...

  3. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    ...(1, 3). The gRNA can be further modified by incorporation of an RNA hairpin, such as the MS2 hairpin, ...with nuclease-competent Cas9. An RNA hairpin incorporated into the truncated gRNA recruits a transcriptional...

  5. Plasmids 101: Protein tags

    Type
    Blog Post
    ... Protein tags are usually smallish peptides incorporated into a translated protein. As depicted in the... are available, but scientists would need to incorporate their recognition sites into their protein tag...

  6. Hot Plasmids - August 2020

    Type
    Blog Post
    ...Find the plasmids at Addgene! CRISPR RNP electroporation and AAV donor infection (CRISPR-READI) enables...GRABNE) sensors allow for sensitive and high spatiotemporal measurements of norepinephrine. These norepinephrine...

  8. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...2024). They developed a new prime editor that incorporates La’s N-terminal domain, PE7 (Figure 2).  PE7...high microglia-specific gene expression when incorporated in an AAV vector (Figure 5). Efficiency and ...

  9. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    ...Xanthomonas campestris   In addition to incorporating broad host range replicons, recombinant vectors...can be expressed. For example, scientists can incorporate interchangeable antibiotic resistance cassettes...

  10. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    ...tumefaciens, a plant-infectious bacteria that can incorporate foreign DNA into plant genomes. Eukaryotes are...stable transfection, DNA from the plasmid is incorporated into the host cell’s genome, so no special sequence...

  11. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    ...synthesis polymerase known to preferentially incorporate A bases across from abasic sites. This approach...a template — in this case, encouraging it to incorporate a new base across from the abasic site before...

  14. Five Popular Model Organisms

    Type
    Blog Post
    ... scientists to easily control the level and spatiotemporal expression of a gene of interest. This type...method that uses heat induction to control the temporal expression of different transgenes, the Stainer...

  15. Tips for Getting a Faculty Position

    Type
    Blog Post
    ...version of my manual, I added new chapters to incorporate the experiences of the people that provided me...interviews. We didn't have Skype in 2004. I also incorporated example documents. This part has generated a...

  17. Evolution of Lab Techniques

    Type
    Blog Post
    ...lengths because extension is terminated upon the incorporation of a ddNTP. Researchers perform four different...activity as fluorescently labeled nucleotides are incorporated, a method called pyrosequencing. The drive for...

  19. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ...individual types of GPCRs with greater spatial and temporal control than is achieved by traditional pharmacologic...unknown.      Figure 3: Chimeric OptoXR incorporating rhodopsin undergoes a conformational change ...

  20. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...obstacle, a positive selection cassette can be incorporated, and used during the differentiation process...homologous recombination, which results in the incorporation of your specific edit into the host genome. ...
Showing: 81 - 100 of 257 results