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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 653 results for: PPL

Showing: 81 - 100 of 653 results

  1. CRISPR Library Amplification

    Type
    Protocol
    ...sufficient quantities of library for experimental applications. Repeated amplifications should be avoided as...

  2. Lentivirus ddPCR Titration

    Type
    Protocol
    ...Safety Cabinet (BSC). Make sure that the BSC is supplied with sufficient pipette tips. Pre-warm the 96-...

  4. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Davidson 54924 mApple-MAPTau-C-10 MAPT mApple CMV Parkinson's, FTD Michael Davidson 54925 mApple-MAPTau-N-10...-10 MAPT mApple CMV Parkinson's, FTD Michael Davidson 55077 mCherry-MAPTau-C-10 MAPT mCherry CMV Parkinson's...

  5. Brain Armamentarium

    Type
    Collection
    .... Useful for nuclear isolation and scRNA-seq applications. Jonathan Ting Viviana Gradinaru 163505-PHPeB...

  6. Deisseroth INTRSECT Collection

    Type
    Collection
    ...EYFP as payloads). This approach has been broadly applied using multiple recombinase-expression strategies...

  7. Bacterial Expression Systems

    Type
    Collection
    ...BiFC studies. ID Plasmid Fluorescent Protein Application PI 14030 14031 pCyPet-His pYPet-His CyPet (donor...

  8. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...

  10. Genetic Code Expansion

    Type
    Collection
    ...single plasmid, with multiple copies of the tRNA. Applications By making small changes in selected amino acids...

  11. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...extensively validated for neuroscience research applications. A substantial number of these have been cloned...antibodies are produced in-house and undergo application-specific validation and quality control by Addgene...

  12. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ... various RNA molecules - such as MS2 stem loops, PPL stem loops, the Spinach2 aptamer, and more - to sequences...potential applications of CRISPR/Cas9 in genome-engineering. One particularly attractive application involves... system widely accessible for many potential applications. Polstein LR & Gersbach CA, Nat Chem Biol ...combinations of states. Each of the integrases is supplied on its own expression plasmid or in various combinations...combinations, while the cognate att sites are supplied as individual reporter constructs or as the fully...amenable to both high and low throughput screening applications. The system can be readily modified to use different...cloning platform for imaging and flow cytometry applications Designing customized fluorescent reporters can...

  13. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    ...assume that their advisor alone applies for these competitive grant applications; however, in some cases, your...the grant application process. When my PhD advisor first asked me to write a grant application due the ...ask your advisor to supply a budget form used in a previously funded grant application to see how funds ...the next round of applications. Step 5: Keep writing! Each written grant application builds upon the ... project grant application" by the NIH Still want more information on the application process? What types...writing, volunteered to take the lead on various applications throughout my graduate career, and helped receive... Step 1: Your PI forwards you a Request For Applications (RFA), what next? First, check the submission...

  14. 6 Tips for Grant Writing

    Type
    Blog Post
    ...good quality application. Even following this advice, only about 50% of my grant applications have been ...do need to be able to research and write your application, then give it to someone else to read and / or...it). It’s easy for reviewers to spot when an application is rushed (whether the idea isn’t properly researched...researched / developed or the application is full of typos) and these are unlikely to get past the initial...someone else in the department) to read over your application and give you feedback. They’ve most likely been...alterations before submitting - handing them your application 3 days before the deadline isn’t going to be ...problem in a novel way. Finally, engineering new applications to solve problems will provide originality to...

  15. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    ...student from applying to the opportunities of their dream.  To help students focus on the application process...internships you are applying to, keep your resume under two pages unless you are applying for a Federal Government...your application a whole week before the deadline. The day of submission, review your application with...COVID-19 pandemic, students will face a reduced supply of internships as a result of the necessary restrictions...Scrutinize every opportunity prior to starting an application to assess your eligibility, interest, and career...February represent peak season for internship applications. Thus these noble individuals are inundated ... with a stellar letter of support.        5. Application submission  Set a calendar reminder for every...

  16. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    ...usually yes! A good cover letter won’t hurt your application, but it can certainly help you stand out.  In...consider cover letters an important part of the application. Even within an organization, there can be variance...good cover letter!) As with any part of the job application process, it's a good idea to talk to people in...do the job as described. Let’s imagine you’re applying to a job that requires ddPCR skills. Your resume... to proactively address questions about your application. These could be logistical, like briefly explaining...  Finally, it’s a good idea to state why you’re applying to the specific company or lab. Take some time...what goes into your cover letter. Imagine you’re applying to a job with a lot of file management that asks...

  17. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    ...developed and applied. The current pandemic serves as a striking example of the applicability and importance...research. I applied, hoping that the experience would improve my scientific and application writing skills...science (as opposed to translational, biomedical, or applied sciences), people conduct research to answer questions...revelation. Science in any field can generally be applied to improve our lives and solve global problems....the people around you. Be proactive Rather than applying only to posted job offerings, the best research...ties into the previous one too. I would recommend applying for multiple positions—don’t put all your eggs...and just lurked online for a while and observed. Apply for those scholarships that you don’t think you’...

  18. 6 Steps to Submitting a Resume That Gets Seen

    Type
    Blog Post
    ...relationships. However, most job seekers will and should apply for jobs posted online as one part of their job ...Utilizing existing relationships as part of the job application process can dramatically increase the chances... of a resume can be crucial for a successful application. It takes time to prepare an excellent resume... Many job postings will result in hundreds of applicants the day they are posted.  If your resume is not... here for more on timely submission of a job application. 3) Read job descriptions carefully Be open minded...have to have all of the listed qualifications to apply for a position.  A job description is usually a ...wish list and the hiring manager knows that no applicant is likely to meet all the criteria. Note the criteria...

  19. Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs

    Type
    Blog Post
    ...received 116,224 employment based green card applications and approved 91,023 during the first 3 quarters...options discussed in this post benefit from the applicant thinking about the process early, keeping good...occupation" as requiring theoretical and practical application of a body of highly specialized knowledge in ... April 1 deadline for companies to submit an application on their behalf.  If an individual obtains a ... cap subject H-1B (last year less than 30% of applicants received sponsorship) then he or she can start...1B petitioners are exempt from the cap if the applicant works at institutions of higher education, a related...NIW) allows for self-sponsorship of the I-140 application meaning that the category does not require a ...

  20. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ...led to their application for basic and translational research.  Basic research applications of scFvs: ...blood clearance, which is useful for imaging applications; better tissue penetration which is useful for... for therapeutic and imaging applications; and reduced immunogenicity when administered in vivo due to...from blood can be a drawback for therapeutic applications where longer retention times often increase ...since it’s genetically encoded.   Translational applications of scFvs: Just like antibodies, there are many...2019).Many of the scFvs designed for oncology applications are reformatted as bispecific molecules which... autoimmune, and/or inflammatory therapeutic applications. Antibody fragments such as scFvs are also used...
Showing: 81 - 100 of 653 results