We narrowed to 612 results for: RET

...classification. Lentivirus (a type of retrovirus) and gamma retrovirus require more caution as they are integrating...components of a virus into different plasmids. For a retroviral system this looks like the genetic cargo in one... researcher.   Figure 1: Separation of retroviral packaging components onto distinct plasmids for...common types of viruses grown in the lab are gamma retrovirus, lentivirus, adenovirus, and adeno-associated...

... synapses, and cytoskeleton components) and in a FRET-based biosensor for cAMP dynamics. The mGold2 variants...administration. AAV11 can transduce neurons for retrograde labeling, making it a powerful tool for circuit...epithelial cells, liver, CNS (limited penetration), retina, hematopoietic cells, and immune cells. B) AAV11... studies have shown that AAV11 enables potent retrograde labeling of projection neurons. Created with ...VAMP2 = synaptobrevin 2; At = ALFA-tag; BACE = β-secretase; AF = AMPA.FingR; FingR = fibronectin intrabody...

...MSCV-IRES-EGFP IRES Retroviral pMSCV-pBabeMCS-IRES-RFP IRES Retroviral pMSCV-IRES-YFP II IRES Retroviral pCMMP-MCS-IRES-Puro...pCMMP-MCS-IRES-Puro IRES Retroviral pEF1a-IRES-Neo IRES Mammalian MSCV-IRES-Luciferase IRES Retroviral pWPI IRES...

...life science literature is a litany of false interpretation in cell culture for this reason. Unreproducible...just a few publications which have since been retracted due to cell line contamination: Epithelial Mesenchymal...even decades after it is initially isolated. Regrettably this is not usually the case if you obtain a ...colleague. Time and money are wasted, papers are retracted and there is a loss of reputation that can impede...working vials have been used up. This ensures you can retain the maximum stocks and use of the original Cell...

...using HR was found to block the acquisition and retrieval of contextual fear memories [5].  In a modified...other regions of the brain may be involved in retrieval of remote contextual memories. This study is an...real-time inhibition), and behavioral (remote memory retrieval) specificity. Optogenetics can be used to more...Twitter @derekpsimonphd.    References 1. Stuber, Garret D., et al. "Excitatory transmission from the amygdala...2012): 159-172.5. Goshen I, et al. Dynamics of retrieval strategies for remote memories. Cell. 2011;147...

... constant variables that enable the correct interpretation of the effect of the independent variable you...controlled for in order to eliminate any alternative interpretation of the results. Typically, plasmid-based experiments...non-targeting control is critical to the correct interpretation of the results because it provides an important...limitations and you should be cognizant of these when interpreting your results. The key to biological controls...multiple replicates, and ultimately getting interpretable results: Figure 4: Expression Level of Gene ...

...determined by the organized smooth endoplasmic reticulum (OSER) assay. A major advantage of mCyRFP1 is ...ability to be coexcited along with EGFP while retaining an emission spectrum that is easily separable ...makes mCyRFP1 a powerful tool for use as a donor in FRET (fluorescence resonance energy transfer) and FLIM...Lin and Yasuda groups use both mCyFP1 and EGFP as FRET donors in experiments simultaneously measuring the...mammalian mCyRFP1 or CyRFP1 expression plasmids, FRET constructs, and CaMKII and RhoA sensors. Laviv,... of ~50% and confirmed that the fusion protein retained the PAM properties of the individual Cas9. Kim...

... Photoreceptor Cells AAV2, AAV5, AAV8 RPE (Retinal Pigment Epithelium) AAV1, AAV2, AAV4, AAV5, AAV8...AAVs may move toward the cell bodies of neurons (retrograde transport) or towards the synapse (anterograde...serotypes 1, 8 and 9, not only do they undergo retrograde and anterograde transport, but they also have... specific cell populations. Find Ready-to-Use Retrograde AAV Preps at Addgene 2) Comparison of genetically...share conserved mechanisms for anterograde and retrograde axonal transport." Human gene therapy 25.8 (2014...

...assaying a secreted protein, first block the Golgi apparatus with Brefeldin A to inhibit secretion and then...expand monoclonal cultures.  Interpreting flow cytometry data To interpret flow cytometry data users will... Kim C, Boulanger CM (2012) Flow cytometry: retrospective, fundamentals and recent instrumentation. Cytotechnology...

...Combinatorial strategies can also be used for retrograde tracing: visualizing neurons that received information...region. In the example shown in Figure 1, injected retrograde AAVs at one location result in opsin expression...viral vector related blog posts Learn more about retrograde AAVs Resources on Addgene.org Read our AAV ...guide Learn more about our viral service Find retrograde AAVs at Addgene ...

...the blue "Save Verified Data and Return to Article" button to return to the table listing all of the plasmids...the full plasmid sequence can be assembled or theoretical — it does not have to be entirely verified by...recombination in bacteria) such as lentiviral, retroviral, and AAV plasmids, we recommend the NEB Stable...process for all of the plasmids in your deposit, return to the Add Plasmids page and click the blue "Submit...

...derived from the E. coli Ec48 retron RT and the S. pombe Tf1 retrotransposon RT, respectively. Their small...years after their initial publication, the Liu lab returned to the reverse transcriptase domain for optimization..., A., Gao, X. D., Raguram, A., Richter, M. F., Everette, K. A., Banskota, S., Tian, K., Tao, Y. A., Tolar...w Nelson, J. W., Randolph, P. B., Shen, S. P., Everette, K. A., Chen, P. J., Anzalone, A. V., An, M., ...

...principle of bioluminescence resonance energy transfer (BRET). Photons emitted by a Renilla luciferase variant...This technique is very similar to the widely used FRET, but it eliminates the need for an excitation light...exhibited 3-5 fold increased brightness over previous BRET probes, with sufficient signal for in vivo and in...Check Out All Our Fluorescent Protein Resources Find FRET Plasmids Find Empty Backbones with Fluorescent Protein...

... be fused to other proteins, and these fusions retained the ability to bind GFP. The idea of using GFP...T-DDOGs, GFP, and a reporter construct into the mouse retina. GFP expression robustly activated the reporter...Cre-DOG is both robust and specific. When tested in retinal electroporation studies, DsRed reporter expression...studies, and since cells infected with AAV-Cre-DOG retain normal neuronal function, this system may make ...

...Smith et al., 2024). They then used single-molecule FRET to study the intramolecular dynamics of the proteins...2: CLIP-SNAP fusion proteins for single-molecule FRET. A) Schematic of a CLIP-SNAP fusion protein (a.k.a...Garcia-Marcos’ lab recently developed a collection of BRET-based ONE vector G-protein Optical (ONE-GO) biosensors...ONE-GO biosensors measure the formation of Gα-GTP by BRET. The biosensor consists of a YFP-tagged G protein...

...cause an effect called FRET (fluorescence [or Förster] resonance energy transfer). FRET occurs when energy...excites the fluorescence of another FP (e.g. YFP). FRET only occurs when the distance between the two FPs...considered when labeling proteins that interact. Indeed, FRET is often used to determine if two proteins interact...

...and stop. For instance (chr3, 1, 10) indicates a stretch of DNA at the very beginning of the third chromosome... Let’s now examine how we can locate powerful stretches of DNA known as enhancers with the help of genome...ESCs) with a lentiviral vector, an attenuated retrovirus in the same family as HIV. By design, our lentivirii...light on dark matter Precise knowledge of which stretches of the genome are pillars for stem cells, or metastasized...

...domain being tested. Find FRET plasmids here. Split EGFP: An alternative to FRET, split EGFP has also been... of GFP. Fluorescence resonance energy transfer (FRET): This is used to study the interactions between...concentrations and pH, using a range of strategies such as FRET, calmodulin, and others. Review Addgene's collection...

...Both PILATeS (18 kDa) and STReTCh (11 kDa) are significantly smaller than FRET-based mechanosensors (~60...be easily expressed and purified from E. coli, retains important functional properties, and is amenable...In this new variation on their previous sensor STReTCh, the 11-amino acid BiT fragment of luciferase is...

... tips from experts on FRET techniques. By combining standard donor/acceptor FRET know-how with aptamers...Aptabeacons Fluorescence resonance energy transfer (FRET) technology has been studied and used in assays ...Additional Resources on the Addgene Blog Tips for Using FRET in Your Experiments Learn more about GFP Controlling...