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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 1,009 results for: RING

Showing: 81 - 100 of 1009 results

  1. Antibody Production

    Type
    Collection
    ...thaw of antibodies and suggests preparing single-use aliquots before storing at -20 °C. Each antibody catalog...production, all antibody preps are titered and undergo stringent quality control by Addgene or a trusted quality...distribution. Details about our production protocols, titering methods, and quality control are described below...Coomassie staining, the sample is separated via a denaturing SDS-PAGE gel alongside serial dilutions of a ...each order based on the total items in the order, ensuring each item ships safely and most economically. ...

  2. Promega Plasmid Collection

    Type
    Collection
    ...interactions, such as exploring viral biology without disturbing the virus of interest and monitoring endogenous ... Plasmid Collection Promega Plasmid Collection Ordering & Availability All Plasmids Plasmid Technology...tags such as NanoLuc, HaloTag, SmBiT, and LgBiT. Ordering & Availability Plasmids from the Promega collection... business days. For more information about the ordering process, check out our help center . Plasmid Collection...sensitivity. These assays are ideal for real-time monitoring of signaling pathways, investigating RNA regulation...

  3. Zinc Finger Consortium: OPEN Reagents

    Type
    Collection
    ...Plamids for Oligomerized Pool ENgineering (OPEN) method has been used to engineer ZFNs that function ...used to engineer ZFNs via the Oligomerized Pool ENgineering (OPEN) method. This kit consists of 5 individually...of Kit Original Publication Oligomerized pool engineering (OPEN): an 'open-source' protocol for making ...Joung et al., PNAS 2000 ). The Oligomerized Pool ENgineering (OPEN) method has been used to engineer ZFNs ...For your Reference section: Oligomerized pool engineering (OPEN): an 'open-source' protocol for making ...

  4. Synthetic Biology - Overview

    Type
    Collection
    ...SynBio Assembly Standards SynBio Blog Genome Engineering SynBio Depositing Labs Hal Alper J. Chris Anderson...Weiss Synthetic biology describes the rational engineering of biological systems for novel functions. Addgene... biologists around the world to facilitate the sharing of parts and information. Addgene can help you ...Regulation Browse plasmids containing both naturally occurring and synthetic regulatory elements, including promoters...Bio-Energy Institute Synberc - Synthetic Biology Engineering Research Center The PLOS Synthetic Biology Collection...

  5. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...re-purposed for site-specific eukaryotic genome engineering. CRISPR/Cas9 is an inexpensive, facile, and efficient...site. Design additional primers to analyze for scarring (small indels produced at the sgRNA cleavage site...to amplify the sgRNA target site to examine for scarring. This may be useful to characterize the non-deleted...Minimize the time between electroporation and transferring the solution into media to enhance cell viability...of Transfected Cells Prepare cells for FACS by filtering them through a 50 μm filter into a FACS tube. ...manuscript here: Bauer et al, 2014 322.2 KB Genome engineering using the CRISPR-Cas9 system. Ran FA...

  6. Antibody Guide

    Type
    Collection
    ...light orange, that bind to a specific antigen, triggering an immune response. The stem of the Y structure...valency of 2 IgG1; IgG2; IgG3; IgG4 IgM Produced during the primary immune response; found mostly in blood...protein:protein complex. Due to their increased manufacturing requirements, monoclonal antibodies are typically...tolerate freeze-thaw cycles. Here are a few tips for storing your antibody: Antibodies conjugated to fluorophores...a similar assay type) and search again. After filtering, narrow down further by company, price, and other...assay with the results of a non-antibody assay measuring either the same output or a proxy for that output... antibody validation This method validates by comparing your antibody of interest to a second, independent...

  7. Immunology Research Plasmids and Resources

    Type
    Collection
    ...DMA, HLADM, RING6 HLA-DMB major histocompatibility complex, class II, DM beta D6S221E, RING7 HLA-DOA major...a pathogen and is the dominant defense strategy during the first several days following exposure to a ...communicate with each other across long distances. During infection and inflammation, the different cytokines...create pores within an infected cell membrane, triggering cell death. TCR Signaling The T cell receptor...macrophages and sense conserved microbial molecules during an infection. When TLRs sense a microbial pathogen...peptidase 7) D6S216, D6S216E, LMP7, MGC1491, PSMB5i, RING10, beta5i PSMC1 proteasome (prosome, macropain) 26S...ABCB2, APT1, D6S114E, FLJ26666, FLJ41500, PSF1, RING4, TAP1*0102N, TAP1N TAP2 transporter 2, ATP-binding...

  8. Neurodegeneration Research Collection

    Type
    Collection
    ...separate diseases, will help speed progress towards curing and treating them. Scientists are studying protein... field will advance more quickly with the avid sharing of data, tools, resources, methods, and results...mid-sixties, or late-onset, where symptoms appear during or after a person's mid-sixties. The early-onset...2014 Apr 17. Next-generation GRAB sensors for monitoring dopaminergic activity in vivo. Sun et al. Nat... an aggressively funded research agenda and to ensuring the development of improved therapies for those...

  9. Plasmid Collections

    Type
    Collection
    ...before starting your experiment. Genome Engineering Genome Engineering Overview CRISPR Cre-lox TALENs Zinc...Kit Types Fluorescent Proteins Cloning Genome Engineering Pathways & ORFs Synthetic Biology Pooled Libraries...plasmids all built with the same backbone and only differing in a small region. Click here to view all pooled...

  10. AAV for Neuronal Tracing

    Type
    Collection
    ...tracing virus that lacks the ability to infect neighboring cells. For example, G-deleted rabies lacks the...starter" cell, but new virus cannot spread to any neighboring cells. To enable monosynaptic spread of this ...newly formed viruses are unable to infect any neighboring cells. Thus, through complementation, G-deleted...released from the starter cell and free to infect neighboring cells via G-protein receptors, which are naturally...

  11. Zinc Finger Consortium: Zinc Finger Arrays

    Type
    Collection
    ... Joung, Randall Peterson, and Joanna Yeh are engineering zinc finger arrays targeted to various zebrafish...zebrafish. Target Gene Name Zinc Finger Arrays Engineering Method Full Target Site (5' to 3') Left half-... publication. References Rapid 'open-source' engineering of customized zinc-finger nucleases for highly...301. PubMed PMID 18657511 . Oligomerized pool engineering (OPEN): an 'open-source' protocol for making ...

  12. Plasmids for Stem Cell Research

    Type
    Collection
    ...differentiate into many different cell types in the body during development. Unlike other cell types, each stem...and Klf4) that are known to maintain pluripotency during embryonic development. This “reprogramming” of ...0214. Cheng RNA Human Reprogramming factors for delivering synthetic mRNAs encoding human Klf4, Oct4, Sox2...plasmids Sequential expression of pluripotency markers during direct reprogramming of mouse somatic cells. Cell...separate plasmids Defining molecular cornerstones during fibroblast to iPS cell reprogramming in mouse. ...

  13. Worm Expression Resources

    Type
    Collection
    ...Genomes - Cold Spring Harbor's guide to RNAi in C. elegans WormAtlas - A database featuring behavioral and...below: SapTrap-SEC kit for C. elegans genome engineering - Bob Goldstein Lab. Contains a set of plasmids...collection of original, peer-reviewed chapters covering topics related to the biology of C. elegans and...

  14. Plant Plasmids and Resources

    Type
    Collection
    ... molecular biology technologies: from genome engineering and gene regulation to fluorescent proteins. ...modification of monocot and dicot genomes using genome engineering; validated in tomato, tobacco, M. truncatula ...for chloroplast transformation and metabolic engineering in plants (including tobacco and potato) - Scott...microRNA and siRNA vectors in Arabidopsis - James Carrington. JoinTRV , an improved vector system based on...

  15. Plan Your Experiment

    Type
    Collection
    ... the edit type, designing and cloning a gRNA, delivering Cas and the gRNA, and validating the edit....gRNAs that have been used successfully in genome engineering experiments. Using validated gRNAs can save your...the gRNA for CRISPR activity. Because you are delivering the mRNA directly to the cells, there is only...References Cong, L., & Zhang, F. (2015). Genome engineering using CRISPR-CAS9 system. Methods in Molecular...Glass, Z., Lee, M., Li, Y., & Xu, Q. (2018). Engineering the delivery system for CRISPR-Based genome editing...

  16. TALEN Plasmids and Kits

    Type
    Collection
    ...Genome Engineering TALENs TALEN Plasmids and Kits You may also like... Addgene's Genome Engineering Guide...Kits TALENs are important new tools for genome engineering. Fusions of transcription activator-like (TAL...Voytas Adam Bogdanove Joung Lab TAL Effector Engineering Reagents Assembly via serial ligation. Validated...

  17. COVID-19 Resources

    Type
    Collection
    ...fluorescent reporter plasmids that have been used for measuring viral entry and activity in neutralization assays...Industry column below. For more information on ordering from a for-profit entity, please see our Plasmid...access to articles related to SARS-CoV-2 research during the current outbreak: Cell Press Coronavirus Resource...Collection Science Journals Coronavirus Collection Springer Nature SARS-CoV-2 and COVID-19 Articles Wiley ...

  18. Zhang Lab CRISPR Page

    Type
    Collection
    ... Genome Engineering CRISPR Zhang Lab CRISPR Plasmids Zhang Lab CRISPR... the CRISPR-Cas9 system can be implemented by delivering Cre and sgRNA to a Cre-dependent mouse or sgRNA... References SpCas9 plasmids Multiplex Genome Engineering using CRISPR/Cas Systems. Cong L, Ran FA, Cox.... 2013 Oct 10;155(2):479-80. PubMed . Genome engineering using the CRISPR-Cas9 system. Ran FA, Hsu PD,...

  19. Individual REAL TALE Repeat Plasmids

    Type
    Collection
    ...be found on the TALengineering website. Users may also wish to join the TALengineering newsgroup . ID ... TALengineering Reagents TALE Repeat Plasmids Individual REAL...

  20. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Parkinson's Kalle Gehring 45970 pGEX-parkin 77-465 PRKN GST tac Parkinson's Kalle Gehring 45971 pGEX-parkin...Parkinson's Kalle Gehring 45972 pGEX-parkin 219-465 PRKN GST tac Parkinson's Kalle Gehring 45973 pGEX-parkin...Parkinson's Kalle Gehring 45974 pGEX-parkin W403A PRKN GST tac Parkinson's Kalle Gehring 45975 pGEX-parkin...Parkinson's Kalle Gehring 45976 pGEX-parkin C431S PRKN GST tac Parkinson's Kalle Gehring 45977 pGEX-parkin...pGEX-parkin W403A-C431S PRKN GST tac Parkinson's Kalle Gehring 46904 pRK5-EGFP-Tau MAPT GFP CMV Parkinson's, FTD...eIF4G delN83 EIF4G1 V5, MS2 CMV Parkinson's Niels Gehring 66818 pCCL-PGK-SPdCas9-BFP-DNMT1 DNMT1 PGK Hereditary... 86-130 Parkin) PRKN GST tac Parkinson's Kalle Gehring 102361 pcDNA3.1-hSNCA-NE SNCA NE CMV Parkinson'...
Showing: 81 - 100 of 1009 results