We narrowed to 237 results for: SPL;

...to produce infectious viral particles have been split into multiple plasmids for safety: Transfer plasmid...

...colleagues are working on developing new surface display vectors to improve expression and trafficking of...: Schematic of iGluSnFR3 (left). GluSnFR3 in pMinDisplay (PDGFR anchoring domain), in electroporated in...

...meant for isolating RNA from rat spleens in which they homogenized spleens in a guanidinium thiocyanate solution...

...Interactions with Split-BioID Find resources for studying chromatin Resources on Addgene.org Find Split-BioID plasmids...

...generated a photosensitive Cre, Opto-Cre-Vvd, by splitting and joining the protein regions with Vivid (Vvd...low and high-intensity blue light. Unlike other split-Cre systems which can take up to 24 hours to cut...

... reverse transcription. The edited DNA strand displaces the unedited 5’ flap and the resulting heteroduplex...

...Mapping antibody-escape mutations with the yeast display library. From Starr et al Science., 2021. SARS-CoV... designed so that the SARS-CoV-2 spike RBD is displayed on the yeast cell’s surface. The Bloom lab used...

...cells with the CRISPR library in vitro before transplanting them into mice. For in vivo screens, mice are...another option is to use variants of Cas9, like split Cas9, or a smaller Cas9 ortholog like SaCas9. You...

...vitro (see figure 1B in Terskikh et al.) and even displays predictable kinetics over 14 hours of expression... table: Fast-FT, Medium-FT, and Slow-FT. These display progressively longer blue fluorescence maturation...

...storage. What we don't have are the keyboards and displays. We don't have good ways of converting typically...is the next priority; we need the keyboards and displays for control and visualization. Addgene: What do...

...ubiquitous or a tissue-specific promoter. cGAL and Split cGAL plasmids - Paul Sternberg Lab. Plasmids for...

...of test compounds is measured by competitive displacement of a NanoBRET tracer, reversibly bound to a ...

...mismatch at that position. PspCas13b-ADAR2DD(E488Q) displayed robust editing with various spacer lengths from...is more precise than shRNA knockdown, it still displays substantial off-target activity. By mutating residues...

...penetration Less resolution due to larger label displacement Require separate incubation of 1° and 2° Must...multiple dyes Greater resolution due to lower label displacement 2° incubation can be skipped by pre-binding ...

...their characterization of two Cpf1 orthologs that display cleavage activity in mammalian cells. Like Cas9...Cpf1 candidates, Zetsche et al. found two that display robust cleavage activity similar to that of Cas9...

...tested. Find FRET plasmids here. Split EGFP: An alternative to FRET, split EGFP has also been used to study...

... cells correlates with the occurrence of post-transplant opportunistic infection in liver and kidney recipients... recipients. World Journal of Transplantation, 8, 23–37. https://doi.org/10.5500/wjt.v8.i1.23 Jhunjhunwala...

...light-sensitive systems. Most excitingly, assembly of the split luciferase is reversible, making PILATeS suitable...., Wang, B., Prakash, M., & Dunn, A. R. (2024). Split Luciferase Molecular Tension Sensors for Bioluminescent...

...is a significant need for healthy livers for transplants. Growth of new livers in culture could resolve..., neither of these can substitute for a liver transplant. My lab will combine bioengineered scaffolds ...

...environmental triggers. This is done by creating a split dCas9 protein that can reassemble when exposed to...015-0784-0 Zetsche B, Volz SE, Zhang F (2015) A split-Cas9 architecture for inducible genome editing and...