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Addgene

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We narrowed to 232 results for: SPL;

Showing: 81 - 100 of 232 results

  1. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    ...penetration Less resolution due to larger label displacement Require separate incubation of 1° and 2° Must...multiple dyes Greater resolution due to lower label displacement 2° incubation can be skipped by pre-binding ...

  2. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    ...mismatch at that position. PspCas13b-ADAR2DD(E488Q) displayed robust editing with various spacer lengths from...is more precise than shRNA knockdown, it still displays substantial off-target activity. By mutating residues...

  3. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    ...their characterization of two Cpf1 orthologs that display cleavage activity in mammalian cells. Like Cas9...Cpf1 candidates, Zetsche et al. found two that display robust cleavage activity similar to that of Cas9...

  5. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    ...is a significant need for healthy livers for transplants. Growth of new livers in culture could resolve..., neither of these can substitute for a liver transplant. My lab will combine bioengineered scaffolds ...

  6. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...light-sensitive systems. Most excitingly, assembly of the split luciferase is reversible, making PILATeS suitable...., Wang, B., Prakash, M., & Dunn, A. R. (2024). Split Luciferase Molecular Tension Sensors for Bioluminescent...

  7. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ... cells correlates with the occurrence of post-transplant opportunistic infection in liver and kidney recipients... recipients. World Journal of Transplantation, 8, 23–37. https://doi.org/10.5500/wjt.v8.i1.23 Jhunjhunwala...

  8. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ...environmental triggers. This is done by creating a split dCas9 protein that can reassemble when exposed to...015-0784-0 Zetsche B, Volz SE, Zhang F (2015) A split-Cas9 architecture for inducible genome editing and...

  9. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...iATPSnFR1.0 and iATPSnFR1.1 (cytoplasmic, membrane-displayed, expression in neurons, astrocytes) A genetically... 1720648115. Katalin Torok Glutamate Membrane displayed variants of fluorescent glutatmate sensors (iGluSnFR1...

  10. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ...popular tools for studying frogs include egg transplants and microinjections. Injections of mRNAs, for...disease-relevant genes in such a controlled environment displays how essential the frog is as a biomedical model...

  11. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...protein-protein interactions, we recommend using NIR split reporters based on miRFPs (Shcherbakova et al., ...and higher contrast than previously developed NIR split probes (Tchekanda et al., 2014, Filonov et al., ...

  12. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ...authors in this blog post. Figure 2: iGluSnFR3 displays enhanced brightness, expression, and sensitivity...from Twitch-2B (Matlashov et al. 2022). iGECInano displays better response kinetics and photochemical characteristics...

  13. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ...to a number of rare genetic diseases caused by splicing defects, including ataxia telangiectasia, congenital...Exon-skipping antisense oligonucleotides to correct missplicing in neurogenetic diseases. Nucleic Acid Ther. ...

  14. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ... effective in both NHPs and mice, but they can display species-specific tropism. For example, AAV9-X1.1...strains). AAV-PHP.eC, AAV9-X1.1 and AAV1-X1 do not display strain-specific tropism and can be used in LY6A-nonpermissive..., lung, and thymus, and transduce the heart and spleen at similar efficacy levels to AAV9 (Stanton et ...

  15. Immunology Research Plasmids and Resources

    Type
    Collection
    ... regulatory factor X-associated protein - SHFM1 split hand/foot malformation (ectrodactyly) type 1 DSS1...oncogene homolog A (avian) MGC131774, NFKB3, p65 SYK spleen tyrosine kinase DKFZp313N1010, FLJ25043, FLJ37489...of sevenless homolog 2 (Drosophila) FLJ25596 SYK spleen tyrosine kinase DKFZp313N1010, FLJ25043, FLJ37489...Fox Foundation is a synuclein phagemid VHH phage display library used to screen for antibodies that bind...

  16. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...versions with both PDGFR and NGR fusions for membrane display, they recommend the NGR vectors for most experiments...biocytin-filled cell expressing BiPVe3-dTomato, displaying characteristic PV basket cell morphology. Adapted...

  17. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ...viewers. Use a colorblind-friendly color palette for display (like green-magenta-blue or orange-blue rather ...>Replace Red with Magenta. Or you can use Color>Split Channels and then Color>Merge Channels into whatever...

  18. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ...tend to be choc-full of open reading frames that display little to no homology to anything else, making ...amplification (by a mechanism known as multiple displacement amplification) even in the presence of certain...

  19. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...during the differentiation process. Moreover, a splinkerette-PCR assay may be used to map the specific genetic.... Berns, and D. J. Adams, “A high-throughput splinkerette-PCR method for the isolation and sequencing ...
Showing: 81 - 100 of 232 results