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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 420 results for: SUA

Showing: 81 - 100 of 420 results

  1. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty...

  2. Protocol - How to Ligate Plasmid DNA

    Type
    Protocol
    ...sticky ends that were generated by EcoRI digestion: Usually, scientists select two different enzymes for adding...Ratio Although a 3:1 insert to vector ratio is usually sufficient, you can optimize the amount of insert...

  3. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty...

  4. CRISPR Plasmids - C. elegans

    Type
    Collection
    ...Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty...

  6. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty...

  7. CRISPR Plasmids - Yeast

    Type
    Collection
    ...Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty...

  8. 6 Tips for Analyzing and Troubleshooting Sanger Sequencing Results

    Type
    Blog Post
    ...proper ladder. These controls help you properly visualize your results.The most important of those is to...comes to DNA sequencing the chromatogram is your visual control. And, like all controls, missing out is...bases of a DNA sequencing read The peaks here are usually unresolved and small, so I suggest designing your...

  9. Antibody Plasmid Collection

    Type
    Collection
    ...protein of choice from a mix of other proteins, visualize a protein under a microscope, or detect when the...

  10. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty...

  11. Promega Plasmid Collection

    Type
    Collection
    ...special functions that vary from purification, visualization, and functional studies. You can even customize...

  12. Pouring LB Agar Plates

    Type
    Protocol
    ... bacteria with resistance to that antibiotic - usually conferred by a plasmid carrying the antibiotic ...H 2 O Sterile plates of your desired size - we usually use 60 mm x 15 mm plates which can hold 5-10 mL...

  13. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    ...detect a given molecule. The sensor component is usually a protein that undergoes a conformational change...Localization within cells Fluorescence microscopy Visualize components of individual cells Separation/identification...Aptamer Fluorophores Use CRISPRainbow for Genome Visualization Resources on Addgene.org Find Biosensor Plasmids...

  14. Antibodies 101: Chimeric Antibodies

    Type
    Blog Post
    ...different secondaries for your antibody pair and visualize both targets in parallel (Fig. 2). For example...secondary to detect Protein B and successfully visualize both proteins.     Figure 2: Two primary...fluorescent labels and both targets can be successfully visualized (bottom panel).    Can any antibody be converted...

  15. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    ...Interactive Fluorescent Protein Property Visualization allows for visual comparison of FPs and how they relate...collaborated with him on a web-based interactive visualization of FP attributes (which turned into www.fpvis.org...

  16. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    ... local exercise spot or find something new. We usually have yoga class twice a week at Addgene HQ, but...presentations. Learn a new skill for analyzing and visualizing your data, such as R or Python. Write a manuscript...idea is not necessarily to chat, but to mimic your usual work environment.  Be kind to yourself. This is ...

  18. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type
    Blog Post
    ...unmeasurable skills and productivity coming from that casual discussion on the way to the office or in the kitchen... flexible work schedule the more we realize how casual chatter is very much missed in the virtual world...widely used and could bring back a taste of that casual kitchen chatter, ultimately sparking new ideas....

  19. Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

    Type
    Blog Post
    ... once allow you to more easily and accurately visualize the full size of your construct. Consider buffer...pouring it. EtBr binds to the DNA and allows you to visualize the DNA under ultraviolet (UV) light. Don’t forget... settles in the gel well and the dyes provide a visual reference point so you can easily assess how far...

  20. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty...
Showing: 81 - 100 of 420 results