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We narrowed to 402 results for: SUA

Showing: 81 - 100 of 402 results
  1. Pouring LB Agar Plates

    Type
    Protocol
    ... bacteria with resistance to that antibiotic - usually conferred by a plasmid carrying the antibiotic ...H 2 O Sterile plates of your desired size - we usually use 60 mm x 15 mm plates which can hold 5-10 mL...
  2. 6 Tips for Analyzing and Troubleshooting Sanger Sequencing Results

    Type
    Blog Post
    ...proper ladder. These controls help you properly visualize your results.The most important of those is to...comes to DNA sequencing the chromatogram is your visual control. And, like all controls, missing out is...bases of a DNA sequencing read The peaks here are usually unresolved and small, so I suggest designing your...
  3. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    ...detect a given molecule. The sensor component is usually a protein that undergoes a conformational change...Localization within cells Fluorescence microscopy Visualize components of individual cells Separation/identification...Aptamer Fluorophores Use CRISPRainbow for Genome Visualization Resources on Addgene.org Find Biosensor Plasmids...
  4. Antibodies 101: Chimeric Antibodies

    Type
    Blog Post
    ...different secondaries for your antibody pair and visualize both targets in parallel (Fig. 2). For example...secondary to detect Protein B and successfully visualize both proteins.     Figure 2: Two primary...fluorescent labels and both targets can be successfully visualized (bottom panel).    Can any antibody be converted...
  5. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    ... local exercise spot or find something new. We usually have yoga class twice a week at Addgene HQ, but...presentations. Learn a new skill for analyzing and visualizing your data, such as R or Python. Write a manuscript...idea is not necessarily to chat, but to mimic your usual work environment.  Be kind to yourself. This is ...
  6. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    ...Interactive Fluorescent Protein Property Visualization allows for visual comparison of FPs and how they relate...collaborated with him on a web-based interactive visualization of FP attributes (which turned into www.fpvis.org...
  7. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type
    Blog Post
    ...unmeasurable skills and productivity coming from that casual discussion on the way to the office or in the kitchen... flexible work schedule the more we realize how casual chatter is very much missed in the virtual world...widely used and could bring back a taste of that casual kitchen chatter, ultimately sparking new ideas....
  8. Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

    Type
    Blog Post
    ... once allow you to more easily and accurately visualize the full size of your construct. Consider buffer...pouring it. EtBr binds to the DNA and allows you to visualize the DNA under ultraviolet (UV) light. Don’t forget... settles in the gel well and the dyes provide a visual reference point so you can easily assess how far...
  9. Technical Design of a Western Blot

    Type
    Blog Post
    ...than nitrocellulose membranes. Pro tip! You can visualize proteins on a PVDF membrane without staining; ...protein (HRP) and alkaline phosphates. HRPs are usually preferred because of their higher specific activity...chemiluminescence enzymes, making it easier to accurately visualize proteins of wildly different amounts in the same...background Direct detection method Less sensitive; usually more expensive Nitrocellulose or low-fluorescence...
  10. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type
    Blog Post
    ...have introduced a GFP protein into your cells (visualized via the FITC channel) and you are trying to see...negative control. You can see how it is easier to visualize these four populations in the histogram plot in...gate off the histograms above, allowing you to visualize your data as a dot plot.  To summarize, if you...
  11. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type
    Blog Post
    ...is a dangerous state for the body to be in and usually self-limiting effects kick in trying to resolve...and Defense With increasingly sophisticated visualization techniques it is possible to follow the interactions.... PubMed PMID: 27913448. 3. Cai, En, et al. "Visualizing dynamic microvillar search and stabilization ...
  12. How to Prepare for an Industry Interview

    Type
    Blog Post
    ...met with several individual and/or small groups, usually starting with the hiring manager. These interviews...setting, a conflict, and a resolution. Stories usually have a theme too, which is something not inherently... 5-6 stories for an interview, since one story usually has more than one theme and can be used to answer...
  13. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    ...has developed a new, plasmid-based biosensor to visualise changes in histone methylation in living cells...in the middle of a FRET pair, which is used to visualise the results. When methylation occurs at the histone-derived...sites. This new technology opens up a new way to visualize changes in gene expression and may lead to the...
  14. An Introduction to Adenovirus

    Type
    Blog Post
    ...April 2022, physicians in Scotland began noticing unusual cases of acute hepatitis in the pediatric population...However, here it appears that the culprit for these unusual cases was either 1) a physiological effect of SARS-CoV...transgene) instead.    Although the late genes are usually present in the transfer plasmid for the majority...
  15. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type
    Blog Post
    ...interests include proteomics, machine learning, and visualization for big biology. GPS for the genome We can think...something like Google Maps to help us identify and visualize addresses. That’s where enhancers and genome math...result is a heterogenous population of stem cells, usually housed in a single flask. Through a bit of stochastic...
  16. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    ...and temporal resolution, making it ideal for visualizing gene expression in vivo.  But what ultrasound...genes that, when expressed, enable cells to be visualized under ultrasound imaging. Expression of ARGs ...first-generation mammalian ARGs (mARGs) enabled ultrasound visualization of in situ mammalian gene expression in deep...
  17. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    ...the time of diagnosis) is about 35 years, but it usually ranges from 20-50 years and sometimes longer. There...Unfortunately, the symptoms of mesothelioma are usually very similar to the symptoms of other diseases,... meaning that by the time it has caught it has usually started to spread (metastasize) and turned into...
  18. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    ...steal all their food from other plants and are unusual enough to be interesting biologically. They have...squid has an extreme number of nerve cells and an unusually high level of RNA recoding in the brain. RNA editing...each other in captivity. Sticklebacks demonstrate unusual parallel adaptation to disparate environments so...
  19. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    ...divided into two halves that are distinguished visually through different patterns and the animal only...the opposite chamber) [2]. Laser activation is usually controlled automatically using motion capture software...electric foot shock that has become associated with a visual or auditory cue. The hippocampus has long been ...
Showing: 81 - 100 of 402 results