...target-specific gRNA in the same cell at the same time. Single plasmids containing both the gRNA and Cas...

...acids, their repeat-variable di-residue (RVD). Ultimately, it is this RVD that determines which single ...

...Sp Cas9 Cut Blasticidin Expresses human codon-optimized S. pyogenes Cas9 protein and blasticidin resistance...

...target-specific gRNA in the same cell at the same time. Single plasmids containing both the gRNA and Cas...

...Rodriguez-Estevez, et al. 2020. Transduction optimization of AAV vectors for human gene therapy of glaucoma...

... pLKO.1 cloning protocol is viewed around 3,000 times each month. I looked this up after trying out the... problem with this is that it may take months of time for your manuscript to get reviewed and edited prior... a protocol as you finish them, turn on pre-set timers for incubation steps, and save the entire process...

..._3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3044) Optimized expression of SYFP2 (yellow fluorescent protein...

...-driven EGFP; can be used for cDNA expression Baltimore 12247 pLVTHM 2nd EF-1a-driven GFP and shRNA under...

... Expression with Strep, His or optimized YFP tags for purification or localization ...

...TTTCCGATGGATGAAGTTattgacgatcttattgGCCTTGAAAACGGGTTTA TIMM22 TAL3578 & TAL3579 TTGTTCTGGGAGGTGCTtttggagtcttcacagCCGGCATTGATGCAAA...

...dCas9-based tool for DNA demethylation. AAV Q&A with Tim Miles: While not technically a research story, I ...couldn't resist adding in this excellent Q&A from Tim Miles on everything from AAV prep to targeting to...

...AAVs” with the director of CalTech’s CLOVER lab, Tim Miles, PhD, we invite you to head on over to our ...our YouTube channel and watch it now! (Bonus: Tim kindly offered to finishing answering all submitted questions...

...students. Many thanks to our guest blogger Tim Herman! TIm Herman is the director of the MSOE center for...This post was contributed by Tim Herman, director of the MSOE center for BioMolecular Modeling and the...become real”.  We sometimes forget that the models we create are around 30 million times larger than the...Genome Visualization Tips for a First Time CRISPR User by a First Time CRISPR User Resources on Addgene....

...former colleagues! I ran into past ASM President Tim Donohue (see below) on my way back from getting a...Research Center and a fellow Rhodobacter enthusiast, Tim also hosted me at his University of Wisconsin lab... annual meeting (ASM Microbe 2016) for the first time. Our goals at the meeting were to network with scientists...this Broad Institute lab is doing creating well-optimized CRISPR screening technology. Addgene got several...

...CapsidsResearchers: Cynthia Arokiaraj; Viviana Gradinaru; Tim ShayMultifunctional Neuronal Fibers: Modulation from...grateful for your writing, your knowledge, and your time. ...

...Gene Type Wildtype Mutant Partial Sequence Codon-Optimized gRNA Other Tags GFP FP other Flag GST His TEV ...Gearhart 44333 pMX_mTBP TBP LTR Parkinson's Marc Timmers 44978 GST-FUS-WT FUS GST tac ALS Robin Reed 44981...

...enriched lentiviral CRISPR libraries developed by MIT's Tim Wang and colleagues, including David Sabatini and...CRISPRs was limited to modification of a few loci at a time,” Wang said. “It was very difficult to screen in...

...without the artifacts sometimes associated with plasmid-based overexpression. Tim Formosa, at the University...—in frame, of course—and start tagging! Yeast-optimized fluorophores for imaging Lee S, et al. (...series of blue, green, and red FPs that are codon optimized specifically for expression in yeast. These tagging...Based on their findings, the authors recommend optimal FP combinations for use in yeast imaging, categorized... output requirements. Select from these yeast-optimized fluorophore tagging vectors for your single or...

...cyto).iATPSnFR2.S29W.A95K.HaloTag AAV5 Biosensor Tim Brown New viral prep pGP-AAV-syn-flex-iGABASnFR2...

...There are digital and analog versions of each, and Tim Lu even compared his "memory" gene codes to a Turing...