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We narrowed to 927 results for: TIM;

Showing: 81 - 100 of 927 results

  1. 5 Uncommon Ways to Gain Career Skills During an Uncommon Time

    Type
    Blog Post
    ...level of intimacy that creates the magic of a safe space. No matter why, now is a great time to find a...work-from-home and just now realizing that we can’t bide our time and wait for it to be over to work on career skills...speaking For almost any science career, there will be a time when you need to speak publicly to communicate your... by now that people get jobs at least 85% of the time because of relationships. It’s much harder to build...

  2. Save Time and Money by Making Your Own Competent Cells

    Type
    Blog Post
    ...this adds up to a significant time savings. You can also save yourself time if you're transforming a plasmid... each week at Addgene, so we need to be cost and time efficient. We do this by making our own competent...antibiotics to give the antibiotic resistance gene time to be expressed.  However, you can skip this incubation...

  3. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

    Type
    Blog Post
    ...accumulated over time? Undergraduates can help to answer this question! The Pathways Over Time project The...The Pathways over Time project provides a framework for these undergraduate research questions. The idea...from a common ancestor ~350 million years ago. Over time, the two genomes have become very different due ...species diverged. Tips on using the Pathways Over Time plasmids The plasmids are used as part of a semester-long...D.M., Ludlow, L.H., & O'Connor, C.M. Pathways over time: Functional genomics research in an introductory... pathways over time...

  4. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

    Type
    Blog Post
    ...only easily identify base editing events in real-time but also enrich for base-edited cells in their experiments...Monitoring APOBEC and Cas9-mediated editing in real time The Harris lab created ACE, a reporter that monitors...deaminase proteins) and Cas9-mediated editing in real time (St. Martin et al., 2018). The ACE reporter is a...canonical base excision repair enzymes. At the same time Cas9n cleaves the opposing DNA strain which results...reporter quantifies APOBEC-Cas9 efficiency in real-time, it relies on DSBs which is not ideal for monitoring...efficiency of APOBEC-Cas9 editisome complexes in real-time (St. Martin et al., 2019). To create the eGFP reporters...sequencing (Standage-Beier et al. 2019). TREE is a real-time, fluorescent based identification system for the...

  5. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

    Type
    Blog Post
    ... pLKO.1 cloning protocol is viewed around 3,000 times each month. I looked this up after trying out the... problem with this is that it may take months of time for your manuscript to get reviewed and edited prior... a protocol as you finish them, turn on pre-set timers for incubation steps, and save the entire process...

  6. 8 Great Depositor Research Stories

    Type
    Blog Post
    ...dCas9-based tool for DNA demethylation. AAV Q&A with Tim Miles: While not technically a research story, I ...couldn't resist adding in this excellent Q&A from Tim Miles on everything from AAV prep to targeting to...

  7. Summer Fun at Addgene!

    Type
    Blog Post
    ...AAVs” with the director of CalTech’s CLOVER lab, Tim Miles, PhD, we invite you to head on over to our ...our YouTube channel and watch it now! (Bonus: Tim kindly offered to finishing answering all submitted questions...

  8. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ...students. Many thanks to our guest blogger Tim Herman! TIm Herman is the director of the MSOE center for...This post was contributed by Tim Herman, director of the MSOE center for BioMolecular Modeling and the...become real”.  We sometimes forget that the models we create are around 30 million times larger than the...Genome Visualization Tips for a First Time CRISPR User by a First Time CRISPR User Resources on Addgene....

  9. Addgene at the ASM Microbe 2016 Conference

    Type
    Blog Post
    ...former colleagues! I ran into past ASM President Tim Donohue (see below) on my way back from getting a...Research Center and a fellow Rhodobacter enthusiast, Tim also hosted me at his University of Wisconsin lab... annual meeting (ASM Microbe 2016) for the first time. Our goals at the meeting were to network with scientists...this Broad Institute lab is doing creating well-optimized CRISPR screening technology. Addgene got several...

  10. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Gene Type Wildtype Mutant Partial Sequence Codon-Optimized gRNA Other Tags GFP FP other Flag GST His TEV ...Gearhart 44333 pMX_mTBP TBP LTR Parkinson's Marc Timmers 44978 GST-FUS-WT FUS GST tac ALS Robin Reed 44981...

  11. Lucky Thirteen: Guest Contributors of 2023

    Type
    Blog Post
    ...CapsidsResearchers: Cynthia Arokiaraj; Viviana Gradinaru; Tim ShayMultifunctional Neuronal Fibers: Modulation from...grateful for your writing, your knowledge, and your time. ...

  13. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    ...without the artifacts sometimes associated with plasmid-based overexpression. Tim Formosa, at the University...—in frame, of course—and start tagging! Yeast-optimized fluorophores for imaging Lee S, et al. (...series of blue, green, and red FPs that are codon optimized specifically for expression in yeast. These tagging...Based on their findings, the authors recommend optimal FP combinations for use in yeast imaging, categorized... output requirements. Select from these yeast-optimized fluorophore tagging vectors for your single or...

  14. New Viral Vectors - Winter 2025

    Type
    Blog Post
    ...cyto).iATPSnFR2.S29W.A95K.HaloTag AAV5 Biosensor Tim Brown New viral prep pGP-AAV-syn-flex-iGABASnFR2...

  16. Hot Plasmids - February 2022

    Type
    Blog Post
    ...set of anti-HA frankenbodies (Zhao et al. 2019), Tim Stasevich and Hiroshi Kimura’s labs have recently...Frankenbodies for modular imaging of cellular targets Optimized genetic screening technique using Cas12a for multiplexed...https://doi.org/10.1038/s41467-019-10846-1.   Optimized genetic screening technique using Cas12a for multiplexed...have expanded the genetic screening toolkit by optimizing a Cas12a-based method for screens. Cas12a’s ability...increase the potential of Cas12a genetic screens by optimizing enzyme activity using an enhanced Cas12a variant...from DeWeirdt, et al. 2021 To validate this optimized genetic screening technique, researchers identified...simplicity for combinatorial genetic screens, and this optimized toolkit furthers Cas12a’s potential in future ...

  17. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

    Type
    Blog Post
    ... to a startup Mike Koeris and fellow co-founders Tim Lu and others first came up with the idea that would...raised $6 million. The biggest challenge during this time was learning to communicate effectively to non-scientific...complications and potential conflicts that waste money and time. Most importantly, “the company does not become ...become real until someone takes the risk full-time and abandons all safety nets.” Raising seed money. “Before...formerly VP Operations of Sample6) for taking the time to speak with us about the biotech startup process...

  18. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

    Type
    Blog Post
    ...Currently, Aggarwal, and colleagues Filip Tomaska and Tim Hanson, are characterizing variants of iGluSnFR3 ...Campus, HHMI where he works on developing and optimizing biosensors in the lab. He has recently developed...sensory input from our extremities. “Here, painful stimuli are processed by a network of excitatory and inhibitory... demonstrate pain response due to blue light stimulation of dual virus-expressing CR neurons. (C) Duration...biosensors.   This screening method allowed Tian to optimize a red‑shifted archaerhodopsin‑derived genetically...

  19. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    ...24336571. PubMed Central PMCID: PMC4089965. 9. Wang, Tim, et al. "Genetic screens in human cells using the...References 1. Cheng, Allen A., Huiming Ding, and Timothy K. Lu. "Enhanced killing of antibiotic-resistant...PMCID: PMC4151723. 2. Doench, John G., et al. "Optimized sgRNA design to maximize activity and minimize...

  20. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type
    Blog Post
    ...Plants. Carola Engler, Mark Youles, Ramona Gruetzner, Tim-Martin Ehnert, Stefan Werner, Jonathan D. G. Jones...to overcome them, and why she spends some of her time engaging with others on Twitter, among other things... transcripts as the ones that people use all the time. Now there are many, so if you want to make a 10...switching to Golden Gate is a conceptual barrier. Sometimes people don’t know how to start. They might want...Most Sainsbury scientists are active on Twitter. Sometimes it’s a good way to find out what’s happening in...experiences, articles about women in science, and the sometimes stupid or uncomfortable things that have happened...say to our readers? Patron: Remember plants. Every time you look at your plate, think about how much that...
Showing: 81 - 100 of 927 results