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...ve added many new tools to our inventory of ready-to-use viral vectors. Here are some of the AAV we have...Testing AAV inventory for the entire collection! We are expanding our entire control AAV inventory to give...Calcium Sensors). We are expanding the inventory of these vectors to more serotypes. We currently package...GCaMP7s. See all our biosensors inventory, and check out our latest vectors below: pGP-AAV-syn-jGCaMP7f-...addition to our inventory of Cre-dependent GABA sensors. See all our GABA sensor AAV inventory here. pAAV.hSynap.iGABASnFR...support by growing our inventory of recombinases and recombinase-dependent vectors. We recently released...pENN.AAV.hSyn.Cre.WPRE.hGH (105553-AAV5) Dre vector AAV phSyn1(S)-DreO-bGHpA (50363-AAV5) Flp vectors pAAV-EF1a-mCherry-IRES-Flpo...

...guarantee certain rights and protections for depositor and requestor alike. That’s why Addgene works closely...closely with TTOs to help protect our depositors’ intellectual property, and to help requesting institutions...California Institute of Technology Cold Spring Harbor Laboratory  Dana-Farber Cancer Institute  Fred Hutchinson...

...block Entry vectors and 5 lentiviral Destination vectors. These vectors can induce combinatorial constitutive...research purposes, retroviral vectors have been developed into ‘split-vector systems’ by separating viral...and expression of these vectors when used in experiments. Retroviral vectors make up a large toolbox ...Gamma-retroviral vs. lentiviral vector systems Gamma-retroviral vectors have a relatively high transduction... many more useful viral vectors available at Addgene: Follow our viral vector blog posts for updates on...tips for using retroviral vectors in your experiments. Gamma-retroviral vectors Popular gamma-Retroviral...Transfer Vectors   Expressing your gene of interest pMKO.1 GFP (Plasmid #10676) Retroviral vector for cloning...

...Therapeutics, co-founded by Harvard Investigators and Addgene depositors David Liu, PhD, Keith Joung, PhD...to Rett Syndrome, this blog post describes the history of Rett Syndrome research, the role of the MECP2...exhibit slow head growth, develop seizures, cardio-respiratory dysfunction, distinctive hand movements, and ...& Bird, 2019) and is a global transcriptional regulator of thousands of genes. Studies have suggested ...neurological deficits observed in Rett Syndrome. Restoring the MECP2 gene by excision of the Stop cassette...precise amount of protein to each brain cell to restore physiological levels. Much research is now focused...researchers developed adeno-associated viral (AAV) vectors for the targeted delivery of a functional MECP2...

...plasmid tools! Thank you depositors! In fact, this year, Addgene depositors quickly stepped up to make... as an example). Depositor Week will be fun for everyone, whether you are a depositor or not. We will ...Tuesday: Depositor features (plasmid deposits and blog posts showcasing the work of depositors) Wednesday...for some Addgene depositor fun facts for a quick way to grasp the big impacts depositors have on science...your calendars for November 16-20, 2020 for #DepositorWeek on Twitter!  That’s today through Friday. There...scientists who make a big impact in open science. Depositors consistently contribute to our collection so ...COVID-19 related plasmids currently in Addgene’s repository which have been requested about 1,500 times from...

... adapted them for use in the laboratory. The discovery of GFP The story of how fluorescent proteins like...Aequorea victoria (Figure 1) in molecular detail. On the underside of the “umbrella” of A. victoria there...turns out, he was right! He began to study the A. victoria gene responsible for encoding GFP, and, in 1992...sequence. Soon after that, in 1994, Prasher’s collaborator Martin Chalfie expressed GFP in exogenous organisms...studying it grew. In particular, studies in the laboratory of UCSD biochemist Roger Tsien resulted in much...

...Science History Rap Battle A great example of science story telling following the unfortunate story of Rosalind...and their importance in understanding biological history. 4. Please Don't Kill My Hive Beautifully filmed...with us. Tom McFadden is a YouTuber and science educator looking for creative ways to implement the next...

...When our Director of Biology, Lianna Swanson, suggested that I might be able to attend the 10 Years of...Oct4, Sox2, Klf4, and cMyc (the so called OSKM factors). As the Yamanaka lab's primary Addgene contact... forward to seeing how the sharing of his OSKM factors have affected the stem cell community – a community...the first clinical trial with iPSCs with his collaborator Takahashi in 2014 – just 8 years after the OSKM...control process alone takes nearly a year. Addgene depositor Juan Carlos Izpsua Belmonte said it took the joint...effort of five post-docs to implant human kidney progenitors into a pig. In stark contrast to recent genome...the scientists I've already mentioned, Addgene depositors Marius Wernig, Kristin Baldwin, Kathrin Plath...

...CombiGEM lentiviral vector backbone pAWp12 (Figure 2). Cloning from the pAWp28 storage vector to the pAWp12 ...addition to the combinatorial expression of other genetic elements including transcription factors (Cheng et...indicated below and pool-clone them into the pAWp28 storage vector (Figure 1): Forward oligo: 5’- CACCGNNNNNNNNNNNNNNNNNNNGTTTGGGTCTTCGAGAAGACCTATTCXXXXXXXXC...barcode, respectively. Once cloned into the storage vector, the pooled, barcoded gRNA library is now ready... an easy-to-use tool to analyze the complex combinatorial genetic networks underlying your favorite biological...screening are vastly useful for mapping the combinatorial genetics that underlie complex regulation in...barcoded gRNA libraries that can be used to combinatorially modify the genome, screen for a particular ...

...Initial experiments exposed S. thermophilus to predatory phages to test if the exogenous DNA would be incorporated...system that utilized various cas genes not only to store a record of invading phages but to also destroy ...race" between phages and prokaryotes; the ultimate victor will be the species who has the strongest will ...

...Collections Gene Vector Core Viral Vectors Gene Vector Core Viral Vectors Lentiviral Transfer Vectors Adenoviral... transfer vectors and generating new viral vectors. Download the Lentivirus Transfer Vector Production...lentiviral and adenoviral vectors read our Lentiviral Vector Guide and Adenoviral Vector Guide Find more plasmids...Lentiviral Vectors deposited by Kazuhiro Oka from the Baylor College of Medicine Gene Vector Core Depositor...Adenoviral Transfer Vectors Resources Kazuhiro Oka and the Gene Vector Core (Link opens in a new window) of ...lentiviral and adenoviral transfer vectors with Addgene. Lentiviral Transfer Vectors Within this set of lentiviral...PGK promoter Adenoviral Transfer Vectors The adenoviral transfer vectors contained within this set are derived...

...Guides Viral Vectors Viral Vector Guides and Plasmids Selecting a Viral Vector Type Introduction to Viral...Viral Vectors Resources Viral vectors are popular tools for delivering genetic information to cells. These...and Plasmids Lentiviral Vector Guide | Lentiviral Plasmids Lentiviral vectors are versatile tools because...the host genome. AAV Vector Guide | AAV Plasmids Adeno-associated viral (AAV) vectors are commonly used ... Gamma-retroviral Vector Guide | Gamma-retroviral Plasmids Gamma-retroviral vectors, often referred to...Retroviral vectors are popular for making stable cell lines or clinical use. Adenoviral Vector Guide | Adenoviral...applications as vaccines. Selecting a Viral Vector Type Each viral vector type exhibits different properties and...

... growth factor receptor 1 (FGFR1)3 αVβ5 and α5β1 integrins3 Hepatocyte growth factor receptor (HGFR)3 ...3 AAV receptor (AAVR)2 Epidermal growth factor receptor (EGFR)3 AAV8 Laminin receptor (LR)3 ... Table 1: AAV capsids and receptors. Capsid Primary receptor Coreceptor AAV1 α2,3-/α2,6 N-linked...Viral Vectors 101: An Introduction to AAV Viral Vectors 101: AAV Variables that Matter Viral Vectors 101...serotypes have a primary cellular receptor and sometimes one or multiple coreceptors, which may be present on ...acids1 AAV receptor (AAVR)2   AAV2 Heparan sulfate proteoglycan (HSPG)3 AAV receptor (AAVR; not ...3 Laminin receptor (LR)3 CD93 AAV5 2,3-N-linked sialic acid4 AAV receptor (AAVR)2 Platelet-derived...

...Addgene blog Viral Vector Elements The Retroviral Life Cycle Popular Retroviral Vectors and Their Use in...  Frequently asked questions about γ-retroviral vectors How do engineered γ-retroviruses differ from lentiviruses...Woodchuck hepatitis virus post‐transcriptional regulatory element; sequence that stimulates the expression...was originally published in 2018, in the Viral Vectors 101 eBook, 1st edition.  References and resources...

...been finding and sharing stories unique to our repository and resources. As editor of Addgene’s eBooks — ...amount of vectors available and number of requests tell only part of the story. Viral vectors are, after...GCaMP-encoding viral vectors available in the repository. GCaMP, a calcium indicator (biosensor, by Addgene...information from requestors and depositors, and identifying trends in our own repository data, giving us...released Viral Vectors 101 2nd edition — I was incredibly excited when we picked our viral vector service as... as one of those stories. Viral vectors are viruses engineered to be safely used to deliver a genetic ...titer. Addgene’s Viral Vector Service Besides being complex to use, viral vectors are also difficult both...

...plasmid. Parts shown in grey are optional cis-regulatory elements.   Key elements of an AAV plasmid...outside of the ITRs does not get packaged in the AAV vector. The cloning capacity between the ITRs is ~4.4 ...plasmid harbors non-coding sequences. Other cis-regulatory elements Next let’s talk about some of the elements...Woodchuck Hepatitis Virus (WHP) Posttranscriptional Regulatory Element (WPRE) forms a tertiary structure when... the Addgene blog Learn more about basic viral vector elements Read about four considerations for the...

...welcome Julie Cicalese to the Addgene Board of Directors. Cicalese served as the Chief Human Resource Officer...School. The addition of Cicalese to the Board of Directors brings human resources experience and insight ...included the Belmont Human Rights Commission and mediator for the Massachusetts small claims court summary... Meet the other members of Addgene's Board of Directors Find careers at Addgene ...

...we introduce you to the Journal of Emerging Investigators, an open-access journal that enables high school... the interview with the Journal of Emerging Investigators Podcast breakdown Intro Chapter 1 - Introduction...Olivia Ho-Shing, and the Journal of Emerging Investigators. Chapter 2 - Getting high school students and... building curricula and developing one-on-one mentorship programs. Chapter 6 and Outro - Interview with...

...proteins from the AAV2 serotype are used to package vector genomes flanked by AAV2 ITRs into a capsid other...Strohl WR (2017) Adeno-Associated Virus (AAV) as a Vector for Gene Therapy. BioDrugs 31:317–334 . https:/...for Targeting and Improving the Efficacy of AAV Vectors. Molecular Therapy - Methods & Clinical Development...Adeno-Associated Virus Genome Interactions Important for Vector Production and Transduction. Human Gene Therapy...

...These are stormy times. It feels like we’re being buffeted by wind and waves and sometimes wonder if ...would be fully open and available to industry requestors as well. This is a time when we need as much ...materials via Addgene. Perhaps if each of many grantors supporting research could provide Addgene with...Initiative was able to step in to be our “port in the storm.”  "Addgene is a unique resource for scientists ...research is accelerating essential research in laboratories all around the world." We thank the Chan Zuckerberg...whatever lies ahead this year. We can see signs of the storm passing. We’ve recently piloted Addgene’s new cloning...cloning grade DNA service, and viral vector distribution is recovering from the pandemic-related slowdown...