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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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In Category

We narrowed to 770 results for: VARS

Showing: 81 - 100 of 770 results

  1. Distribution to Industry

    Type
    Collection
    ...Garcia-Marcos 10 plasmids for detecting GPCR activity in a variety of cell types. Protein Purification Optimization...

  2. AAV Molecular Tools

    Type
    Collection
    ...Jonathan Long Overexpression Tools These AAV encode various genes for overexpression. ID Name Expression System...

  3. DNA Quantification

    Type
    Protocol
    ...Molecules absorb different wavelengths of light to varying degrees and most have a specific wavelength that...spectrophotometer between samples if they are expected to vary significantly in concentration. DNA dissolved in...in water is going to have a greater variance in concentration readings than a DNA sample dissolved in ...

  4. Microbiology Resources

    Type
    Collection
    ... E. coli or yeast plasmids? Addgene has a wide variety of tools for work in these model organisms. See...

  5. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...co-express a Cas protein can be paired with a wide variety of Cas-containing plasmids. ID gRNA Plasmid Promoter...

  6. Lentiviral Prep Service

    Type
    Collection
    ...viruses can be used to interrogate the genome in a variety of ways. Cas9 proteins can be guided to specific...

  7. Fluorescence Titering Assay

    Type
    Protocol
    ...developed for 293T cells but can be adapted to a variety of target cell lines and selection markers. Note...FBS can promote or inhibit transfection. Test a variety of brands and lots of FBS to find one suitable ...viability and transduction efficiency. Titer will vary between cell lines. It is not recommended to subject...

  9. CRISPR Plasmids - Plants

    Type
    Collection
    ...co-express a Cas protein can be paired with a wide variety of Cas-containing plasmids. ID Plasmid Promoter...

  10. Molecular Biology Protocol - Restriction Digest of Plasmid DNA

    Type
    Protocol
    ... enzymes, allowing scientists to target a wide variety of recognition sequences. For a list of many commonly... 1 µg of DNA. The total reaction volume usually varies from 10-50 µL depending on application and is largely...is due to "Star Activity" and can happen for a variety of reasons, including high glycerol concentration...

  11. General Transfection

    Type
    Protocol
    ...FBS can promote or inhibit transfection. Test a variety of brands and lots of FBS to find one suitable ...new batch of 1 mg/mL PEI prepared. There may be variation between batches of PEI depending on the user, ...transfect cells with a fluorescent plasmid using a variety of ratios. Check the cells 1–2 days after transfection...

  12. Viral Production

    Type
    Collection
    ...control (QC). The specific QC experiments performed varies for each viral lot. To learn which specific QC ...

  13. Colony Formation Titering Assay

    Type
    Protocol
    ...developed for A549 cells but can be adapted to a variety of target cell lines and selection markers. Note...FBS can promote or inhibit transfection. Test a variety of brands and lots of FBS to find one suitable ...viability and transduction efficiency. Titer will vary between cell lines. It is not recommended that lentiviral...

  14. Ras Pathway

    Type
    Collection
    ...all cells and all tissues. Ras can activate a variety of pathways, which ultimately turn on genes involved...

  15. Plasmid Cloning by Restriction Enzyme Digest (with Protocols)

    Type
    Protocol
    ...approximately 1:3. Since the number of base pairs for each varies, it is difficult to calculate this based on DNA...for each plasmid you are trying to create, with varying ratios of recipient plasmid to insert. It is also... plating on the appropriate antibiotic and try varying the amount of recipient plasmid to insert in the...

  16. Control AAV Preps

    Type
    Collection
    ...Promoter CAG CaMKIIa CMV Dlx EF1a/nEF GFAP and variants Synapsin TBG Other Fluorophore/Tag Green Red None...

  17. AAV Titration by qPCR Using SYBR Green Technology

    Type
    Protocol
    ...fluctuations and to minimize well-to-well variability that result from a variety of causes, such as pipetting error...multichannel pipet to minimize pipetting error and variability Mix samples very well by pipetting back and forth...

  18. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...P8833 Target cells Varies based on your experiment Culture media for target cells Varies based on your experiment...to 20 μl serum-free OPTI-MEM TIP: You may want to vary the ratio of shRNA plasmid, packaging plasmid, and...experiment Materials for assay Varies based on your experiment Detailed protocols for preparing polybrene...

  19. Plasmid Cloning by PCR (with Protocols)

    Type
    Protocol
    ...approximately 1:3. Since the number of base pairs for each varies, it is difficult to calculate this based on DNA...for each plasmid you are trying to create, with varying ratios of recipient plasmid to insert. It is also...your transformation worked. You could also try varying the amount of recipient plasmid to insert. Isolate...

  20. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ...optimal concentration of primary antibody to use will vary and needs to be empirically determined. If possible...buffer. Pro-Tip The ideal antibody concentration will vary and needs to be empirically determined. We suggest...concentrations. The ideal range of the standard curve will vary between targets and needs to be empirically determined...
Showing: 81 - 100 of 770 results