We narrowed to 128 results for: actin

1. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

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   ...active protein binding domain of Phytochrome B interacting factor 6 (PIF6APB), respectively. The cofactor...

2. Transferable Skills Guide: Problem Solving

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   ... description, but this skill is not just about reacting to technical issues. This skill also comes into...

3. RaPID Detection of RNA-protein Interactions

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   ...requires 16-18 hours to sufficiently biotinylate interacting proteins, which results in poor spatiotemporal...

4. Antibodies 101: Selecting the Right Antibody

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   ...isolate an antibody’s immune complexes and confirm interacting partners by mass spectrometry (Uhlen, 2016). ...

5. Click Biology: How to Click and Play to Explore Biology

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   ...biology. Slow reactions are only good enough if the reacting parts are present at high concentrations, but ...

6. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

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   ...tumor, proving once again that a tumor is always interacting with its surroundings and being shaped by them...

7. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

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   ...permits it to bind to the CIB1 (cryptochrome-interacting basic-helix-loop-helix) protein. After fusing...

8. Viral Vectors 101: Chemogenetics

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   ...specific neuron firing are better equipped using fast-acting optogenetic tools. The advantages provided by chemogenetics...

9. Antibodies 101: Choosing the Right Isotype

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   ...tend to be complex with a variety of proteins interacting. Experiments tend to be equally complex with ...

10. A Guide to Designing a Scientific Poster: Content, Layout, and More

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    ...presenting. The latter is catchy, but beware. A distracting background or clashing colors can take away from...

11. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

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    ...behind from a preceding animal (ex: urine) can be distracting. If it is not cleaned, animals tested towards...

12. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

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    ...modifying other sites in the genome and directly interacting with transcription machinery.   Well, I’...

13. Progress Towards a PAM-Free CRISPR

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    ...through structure-guided engineering of the PAM-interacting domain of Cas9. After multiple rounds of engineering...

14. 5 Tips for Troubleshooting Viral Transductions

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    ...more than once. Fibronectin is another membrane-interacting protein that can be used to enhance transduction...

15. Illuminating Epigenetics with A FRET Based Biosensor

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    ...prevents the cellular machinery from physically interacting with the genes in the compacted region and results...

16. xCas9: Engineering a CRISPR Variant with PAM Flexibility

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    ...SaCas9 with an NNGRRN PAM by mutagenizing the PAM-interacting domain. However, these alternative PAM sequences...

17. Truncated gRNAs for Regulating Gene Expression

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    ...Schematic of native (nuclease-competent) Cas9 interacting with either a full-length gRNA (20nt complementarity...

18. SciComm: Taking Science to Elementary and Middle Schools

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    ...schools have dedicated science teachers. I tried contacting our school district’s science curriculum coordinator...

19. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

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    ...final concentration calculation and potentially impacting downstream experiments. The usual suspects, such...

20. A Career in Grants: How To Become a Grant Professional

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    ...up-to-date on the changing guidelines and regulations impacting grant management, we track grant opportunity deadlines...