RNA Extraction Without A Kit
Type
Blog Post
...clean RNA, it’s important to make sure that when extracting, to only take the aqueous layer to avoid carryover...
We narrowed to 129 results for: actin
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Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics
TypeBlog Post...active protein binding domain of Phytochrome B interacting factor 6 (PIF6APB), respectively. The cofactor... -
Transferable Skills Guide: Public Speaking
TypeBlog Post...doesn’t know the term “diving board” so you end up acting it out in front of a very confused group of visiting... -
The Breast Cancer Microenvironment: A Tumor’s Backstage Team
TypeBlog Post...tumor, proving once again that a tumor is always interacting with its surroundings and being shaped by them... -
Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute
TypeBlog Post...sucked to work remotely. I was also tired of the contracting life where you do a little bit of work for one... -
RaPID Detection of RNA-protein Interactions
TypeBlog Post...requires 16-18 hours to sufficiently biotinylate interacting proteins, which results in poor spatiotemporal... -
All in a Twist: dsRNA
TypeBlog Post...handle most of these intermediates, with each one acting on specific substrates and in distinct cell cycles... -
Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation
TypeBlog Post...modifying other sites in the genome and directly interacting with transcription machinery. Well, I’... -
Progress Towards a PAM-Free CRISPR
TypeBlog Post...through structure-guided engineering of the PAM-interacting domain of Cas9. After multiple rounds of engineering... -
Viral Vectors 101: Chemogenetics
TypeBlog Post...specific neuron firing are better equipped using fast-acting optogenetic tools. The advantages provided by chemogenetics... -
Antibodies 101: Choosing the Right Isotype
TypeBlog Post...tend to be complex with a variety of proteins interacting. Experiments tend to be equally complex with ... -
Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools
TypeBlog Post...permits it to bind to the CIB1 (cryptochrome-interacting basic-helix-loop-helix) protein. After fusing... -
5 Tips for Troubleshooting Viral Transductions
TypeBlog Post...more than once. Fibronectin is another membrane-interacting protein that can be used to enhance transduction... -
A Guide to Designing a Scientific Poster: Content, Layout, and More
TypeBlog Post...presenting. The latter is catchy, but beware. A distracting background or clashing colors can take away from... -
Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors
TypeBlog Post...behind from a preceding animal (ex: urine) can be distracting. If it is not cleaned, animals tested towards... -
Illuminating Epigenetics with A FRET Based Biosensor
TypeBlog Post...prevents the cellular machinery from physically interacting with the genes in the compacted region and results... -
xCas9: Engineering a CRISPR Variant with PAM Flexibility
TypeBlog Post...SaCas9 with an NNGRRN PAM by mutagenizing the PAM-interacting domain. However, these alternative PAM sequences... -
Truncated gRNAs for Regulating Gene Expression
TypeBlog Post...Schematic of native (nuclease-competent) Cas9 interacting with either a full-length gRNA (20nt complementarity... -
What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry
TypeBlog Post...final concentration calculation and potentially impacting downstream experiments. The usual suspects, such... -
SciComm: Taking Science to Elementary and Middle Schools
TypeBlog Post...schools have dedicated science teachers. I tried contacting our school district’s science curriculum coordinator...