We narrowed to 110 results for: chm

1. Tips for CRISPR Gene Editing in Mice

   Type

   Blog Post

   ...gRNA targets in that region. The CRISPR Software Matchmaker can be used to determine the pros and cons of...

2. TALEN Plasmids and Kits

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   Collection

   ... selection cassettes on pTAL7a and pTAL7b for enrichment of double-transfected mammalian cells. In addition...

3. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

   Type

   Blog Post

   ...Quaye, M., Kinnon, C., Ancliff, P., Webb, D. K., Schmidt, M., von Kalle, C., Gaspar, H. B., Thrasher, A....

4. Plasmids 101: Biotinylation

   Type

   Blog Post

   ... & Cronan, J. E. “Molecular Biology of Biotin Attachment to Proteins; Symposium: Nutrition, Biochemistry...

5. An Integrin Antibody Toolkit from IPI

   Type

   Blog Post

   ...ligand-binding pocket, their sheer size impeding ligand attachment. The five other IPI rAbs bind RGD-binding integrins...

6. Adeno-associated Viruses (AAVs) for Genome Editing

   Type

   Blog Post

   ...efficiency of homologous integration via gene trap enrichment, whereas pAAV-TK-Acceptor has a conventional ...

7. Multiple Plasmids at a Low Price: Kits from Addgene

   Type

   Blog Post

   ...carefully chosen to perform statistically relevant benchmarking of cell-free expression systems, and tested ...

8. Plasmids 101: Dimers and Multimers

   Type

   Blog Post

   ...18607781. Schleef M, Baier R, Walther W, Michel ML, Schmeer M. Long-term stability study and topology analysis...

9. Plasmids 101: Repressible Promoters

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   Blog Post

   ... 30. PMID: 23995928; PMCID: PMC3811519.Seo SO, Schmidt-Dannert C. Development of a synthetic cumate-inducible...

10. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type

    Blog Post

    ...of replication in Nosek and Errington 2017 and Schmmidt 2009, but for our purposes we’ll call a study ...

11. Technique: Probe Phage Genomes for Host Binding Proteins

    Type

    Blog Post

    ...26761028. PubMed Central PMCID: PMC4728577. 4. Schmitz, Jonathan E., et al. "Rapid DNA library construction...

12. Generating Mouse Models Using CRISPR/Cas9

    Type

    Blog Post

    ...fertilized mouse eggs, circumventing the need for enrichment and selection offered by mouse ESCs. For example...

13. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type

    Blog Post

    ... Smith HE, Lee C-YS, Calidas D, Lu T, Smith J, Schmidt H, Krause MW, Seydoux G (2014) Scalable and Versatile...

14. When Fidelity Matters: A frank discussion about ligase fidelity

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    Blog Post

    ...rare mutations based on ligase reaction and bead enrichment." Analytical biochemistry 434.1 (2013): 34-38...

15. Sequencing Options for CRISPR Genotyping

    Type

    Blog Post

    ...al. 2015) and direct in situ breaks labeling, enrichment on streptavidin and next-generation sequencing...

16. Retrograde AAV viral preps

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    Collection

    ...Cre-dependent Molecular Tool Heintz , Nectow , Schmidt No available items found to match all requested...

17. The Pleiades Promoter Project

    Type

    Collection

    ...J., O'Connor, K., Palma, B., Palmquist, D. L., Schmouth, J. F., Swanson, M. I., Tam, B., Ticoll, A., Turner...

18. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type

    Blog Post

    ...S, Bietz A, Waldhauer MC, Börner K, Fakhiri J, Schmelas C, Dietz L, Grimm D, Correia BE, Eils R, Niopek...

19. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type

    Blog Post

    ....1038/srep19452 Selle K, Fletcher JR, Tuson H, Schmitt DS, McMillan L, Vridhambal GS, Rivera AJ, Montgomery...

20. Antibody Guide

    Type

    Collection

    ...recognize the protein in its native conformation. Attachment of the antibody to the beads is typically done...