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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 267 results for: gnal

Showing: 81 - 100 of 267 results

  1. Antibodies 101: Conjugation

    Type
    Blog Post
    ...various labels to antibodies that produce detectable signals like light or color. In this post, we’ll go over...is not visible or capable of producing a visible signal, but it has a strong affinity for avidin and streptavidin... molecule. These properties allow for efficient signal amplification, making biotin conjugated antibodies...

  2. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    ...one minute BASU generated a strong streptavidin signal (~250-fold enrichment) when compared to a scrambled...scrambled RNA motif control). BioID2 had no detectable signal at the same time point. E. coli’s BirA* was tested...timescale, such as during drug action or cell signaling. Limitations of RaPID Like other BirA*-based proximity...

  3. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    ...polyclonal, as their function is to amplify the signal from a primary antibody; the ability of polyclonal...their target protein thereby greatly increasing signal amplification.  When to stay away from polyclonal...positive control across experiments to be aware of signal strength. In general, polyclonal antibodies produce...

  4. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    ...at Addgene! Knock-in: Introducing tags or stop signals Once Cas9 generates double-strand breaks, a new...2021). For example, inserting a polyadenylation signal downstream of the transcription start site will... insertion of a synthetic polyadenylation (spA) signal. Figure and caption adapted from Hazan and Bester...

  5. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    ...rounds of stripping and reprobing, maximize your signal by starting with the least-abundant protein and...your fluorescent probe. Then, if you don’t see any signal, you’ll want to check for the primary antibody ...that you’re looking for a “blank” result! If some signal remains, you may want to repeat your stripping ...

  6. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    ...FRET signal. Subsequent demethylation separates the fluorescent units and so lowers the FRET signal back...a large role in turning genes ‘off’ and ‘on’, signaling which genes should be ‘read’ by the cellular machinery...

  7. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...mark pixels used to measure signal along the dendrite. D) Fluorescence signal as a function of the distance...profiling or interrogation of context-dependent signaling in primary cells. The authors used this platform...

  8. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type
    Blog Post
    ...fluorophore. For example, in figure 1, the FITC signal is spilling over into the primary detector for ...are a convenient tool to obtain a strong positive signal for a given fluorophore, as they contain a mixture...convenient in that they provide a strong positive signal, even for rare cellular markers, beads can sometimes...

  9. Of Myc and Men

    Type
    Blog Post
    ...neighboring sequence context. Sci Signal 13. https://doi.org/10.1126/scisignal.aax9730  Additional resources on...90746-4 Munro S, Pelham HRB (1987) A C-terminal signal prevents secretion of luminal ER proteins. Cell...

  10. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    ...is continuously detected via a fluorescent signal. The signal increases proportionally to the amount of...concentration of the starting template DNA the fluorescent signal throughout the reaction is compared to a standard...

  12. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    ...immunodetection tools is their strong signal strength. This signal strength is due in large part to their... be labeled with up to 3 dyes, yielding similar signal strength to a standard IgG secondary antibody (...

  13. MXS Chaining

    Type
    Blog Post
    ...proteins, promoters, enhancers, polyadenylation signals, inducible gene-expression sequences, and more....promoter (to drive high-level expression) and a polyA signal (to terminate transcription) and the four constructs... containing multiple identical polyadenylation signals or promoters Gibson et al., 2009 MXS-Chaining...

  14. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...mScarlet-I3 also offered clearer signals due to reduced background signal when tested in more highly autofluorescent...external light source, they have lower background signal and can be used in light-sensitive systems. Most...

  15. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...new improvements. The new variant has increased signal-to-noise ratio and better ability to record subthreshold...they are negative-going sensors (with fluorescent signal at baseline voltage), the investigators found that... SAS, splice acceptor site; pA, polyadenylation signal. Image reused from Riedmayr et al. 2023 under a...

  16. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    ...fluorophore-linked antibodies are used to visualize RNA. Low signal-to-noise ratios with such techniques can be problematic...fluorophore and subsequently leading to a positive signal. The same effect can be achieved for a fluorophore...than separate them (Fig. 1b). One can also make “signal-off” biosensors in which the aptamers fluoresce...

  17. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

    Type
    Blog Post
    ...Microscopy-based lineage tracing can help illuminate how signaling pathways—such as those regulated by one-eyed pinhead—contribute... study also showed that in the absence of the signaling pathway regulated by one-eyed pinhead, incorrect...transform zygotes into adults and explain how signaling pathways control this process. Additional zebrafish...

  18. Hot Plasmids - August 2020

    Type
    Blog Post
    ...found that adding a triple nuclear localization signal or including fusions with Histone 2A or 2B can ...biotinylation via BirA and AviTAG. pMAK contains the signal peptide of MBP, followed by the desired nanobody...NB) sequence, an AviTAG, and 6xHis tag. The MBP signal peptide allows the NB to be exported to the periplasm...

  19. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    ... Fluorescent Protein Fusions Observing a bright signal for the first time after cloning an FP fusion with...illustrated the quantitative increase in fluorescent signal achieved when utilizing an optimized moxFP as opposed... opposed to standard FPs. The increase in signal is not due to a brighter FP, but rather because of the...

  20. Bacterial Expression Systems

    Type
    Collection
    ...protease cleavage sites. Other protein tags and signal peptides are used to enhance protein solubility...purification. Commonly used tags, cleavage sites, and signal peptides include: Epitope tags: 6xHis, Flag, Strep...TEV protease, factor Xa, enterokinase, thrombin Signal peptides for periplasmic localization: PelB, MalE...
Showing: 81 - 100 of 267 results