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We narrowed to 264 results for: his-tag

Showing: 81 - 100 of 264 results

  2. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ...guest blogger, Erik Snapp! Erik Lee Snapp received his PhD in Molecular Microbiology and Immunology from...from Oregon Health Sciences University, did his postdoctoral fellowship with Jennifer Lippincott-Schwartz...Research Campus of the Howard Hughes Medical Institute. His interests include the quality control of secretory... be needed to achieve your intended next career stage and create a timeline for achieving these goals....publications (published, not in preparation) A history of successful funding (fellowship grants) A project...

  3. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    ... in the making by Dr. Zhuhao Wu and his colleagues! Dr. Wu and his lab members (like me!) are dedicated...compatible with the desired applications (such as immunohistochemistry (IHC) or western blot), and cost-effective...rAbs are a relatively new technology that takes advantage of DNA sequencing to turn mAb clones into expression...

  4. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

    Type
    Blog Post
    ...supervisor’s management style, bring challenges to his or her attention as soon as you can. It is in your...you communicate your ideas assertively, listen to his or her viewpoint, and discuss options for mutually...people: Hostile-aggressives, who are usually antagonistic and impolite, Complainers, who spend most ...

  5. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    ...Cruz where his group focuses on gene regulation in nematode molting and spermatogenesis. His group also...conditionally manipulate proteins. Learn more at his webpage or follow him on Twitter.   References Arribere...-CRISPR and co-conversion approaches have the advantage of being used in any genetic background, but require...

  6. Congratulations, Deck The Lab winners!

    Type
    Blog Post
    ...Matthijs Dorst’s Deck the Lab Nativity scene below his Christmas tree! It is that time of the year again...showcase it all!           View this post on Instagram                       A post shared...dyed with real microbial pigment. Check out the hashtag to see the description of all the ornaments. #DeckTheLab...

  7. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...measurable through an epitope tag such as FLAG/His/GFP. Fluorescent protein tags, which correlate closely ...different epitopes. Detection of overexpressed (tagged or untagged) protein When expression plasmids are available...population (pink histogram) compared to the WT cells (green histogram). The unfilled histograms show cells ...400,000 cells were stained with the indicated Synaptotagmin-1 antibodies and corresponding Multi-rAb CoraLite...You can see an example of their work on their synaptotagmin 1 page. The data produced is available on the...interpretation of your results. Therefore, taking advantage of antibody validation efforts and information...to selecting high-performing antibodies for Synaptotagmin-1 (Uniprot ID P21579) for use in western blot...

  8. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    ...University. Max Shen is a Ph.D. candidate at MIT. His research uses applied machine learning and statistical...synthesis are four examples of technologies pioneered in his laboratory. Learn more here. Additional resources...effect on plasmid requests Before we began any sophisticated analyses, we first visualized our data in various... 5: Inferred effects. Values are the inferred percentage of normal activity (the average rate of unique...

  9. Inntags: Innovative Protein Epitope Tagging

    Type
    Blog Post
    ...innocuous tags” (inntags) less likely to alter a protein’s properties. Why do we need new epitope tags? Short... looking to a tag a protein, inntags IT5 and IT6 are a great place to start. These tags are easy to use... about other Protein Tags in our Plasmids 101 Series. Get the Inntag Plasmids Tag endogenous proteins ...major caveat - a tag may interfere with protein localization and/or function, so each tagged protein must ...proteins found that 20% of tagged proteins did not localize similarly to their untagged counterparts. These ...the effects of GFP fusions to inntags or traditional FLAG, HA, and Myc tags. Impressively, IT6-GFP did not...of the traditional tags were suitable for both applications. To prove that inntags are indeed innocuous...

  10. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    ..., modular plasmids, combining fluorescent tags, epitope tags, protease sites, and selection markers. These...fluorophore tagging vectors for your single or multi-color imaging experiments. Interested in epitope tags? Others...have genome tagging systems enabled you to advance your research? Do you have a favorite tagging system that...epitope tag (12xHis, 2xStrep, 3xFlag, Protein A, or V5), and selection marker (KanMX, HphMX, or His3MX).  ...Researchers have long taken advantage of this natural process to integrate protein tags into the genomes of S...These tagging vectors are based on previously described pFA6a-link vectors and include a Kan, SpHIS5, or...especially useful in protein complex studies where tagging of multiple protein products is desired, as multiple...

  11. Cre-lox system

    Type
    Collection
    ...Mammalian Sato 123128 pET-His-Cre 6xHis-Cre T7 Bacterial Liu 123131 pET-His-VCre 6xHis-VCre T7 Bacterial Liu...hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR Cre, KASH-tagged EGFP, and sgRNA expression hSyn AAV Zhang 60820...9xGGS-Cre-6xHis Cre fused to highly cationic GFP variant T7 Bacterial Liu 62939 pET-Cre-6xHis Cre-6xHis T7 Bacterial...73472 RabV CVS-N2c(deltaG)-mCherry-P2A-Cre Cre CMV RABV Jessell 73474 RabV CVS-N2c(deltaG)-Cre Cre CMV RABV...Simpson 106368 pCMV-Tag2B-NCre N-terminal split-Cre CMV Mammalian Hirrlinger 106369 pCMV-Tag3B-CCre C-terminal...106370 pCMV-Tag2B-NCreERT2 N-terminal split-Cre-ERT2 CMV Mammalian Hirrlinger 106371 pCMV-Tag3B-ERT2CCre ...homology arms Mammalian Heller 113837 pCAGGS-mTagBFP2-T2A-iCre TagBFP, iCre CAG Mammalian Capecchi 113849 pCAGGS-pac-T2A-iCre...

  12. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    ...in Molecular Plant Sciences since December 2020. His research focuses on the efficiency of transposon-... with low efficiency.       Sheng Yang obtained his Ph.D. from Shanghai Institute of Biochemistry, Chinese...the newly generated pEcCas/pEcgRNA system.   Advantages of the pEcCas/pEcgRNA system Compared with the...conclusion, the MUCICAT system has the following advantages: Fast and site-specific multicopy chromosomal...

  13. Science Communication for Everyone

    Type
    Blog Post
    ...minorities to study STEM subjects. Here, Sadangi and his team answer a few questions about The Addictive Brain...sessions led by scientists, which is hosted on our Instagram channel every Sunday. What's planned for the upcoming...participate in our one-hour Live Q&A session on Instagram every Sunday. Undergraduate and graduate students...background. Social media is a powerful tool. Use hashtags effectively. This would help you direct your content...

  14. HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

    Type
    Blog Post
    ...colocalization of GFP tagged HA frankenbody with an mCherry, 4xHA tagged histone protein (H2B) in U2OS...could also bind a 1xHA tagged histone protein as well as a mitochondrial protein tagged with spaghetti monster...other fluorescent proteins and tags including mCherry, HaloTag and SNAP-tag for multi-color imaging. Find... the 10xHA tag increased the fluorescent signal to noise ratio five fold over the 1xHA tag.  Lastly, the...use GFP to image nascent proteins. In addition GFP tags are large, dim, and permanently attached to a protein...limitations, scientists have co-opted antibodies for tagging purposes. Different antibodies bind different protein... called epitopes and scientists can use these to tag their proteins of interest. Antibody based probes...

  15. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

    Type
    Blog Post
    ...transgene expression. Which tag should I use? When deciding on the appropriate tag, the experimental design...fluorescence or immunohistochemistry methods, these proteins are routinely fused to ‘tag’ or ‘label’ proteins...our studies suggest that non-fluorescent tags may be advantageous over fluorescent proteins when the fluorescence...detection. Fluorescent proteins are most widely used tags because they emit endogenous fluorescence that allow...protein (GFP) and mCherry, two of the most common tags currently used, are approximately 240 amino acids...most of them were still cytoplasmic. Use of ‘small’ tags to identify proteins Given the limited expression...monkey neurons, we next hypothesized that a different tag fused to the hM4Di might improve the transport of...

  17. Editor's Choice, August 2016

    Type
    Blog Post
    ...techniques researchers are using. The detailed results of his survey can be found in the full post but TLDR: many...optimize and troubleshoot the system. Site Directed Mutagenesis by PCR & Single Base Editing with CRISPR For ...technology may make some uses of Site Directed Mutagenesis by PCR obsolete! Honorable mentions Of course... in chronological order here: Site Directed Mutagenesis by PCR Google Forums Round Up: First Impressions...

  18. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ... for epitope tagging: Inntags When fused to your protein of interest (POI), epitope tags give you the...production of a new series of epitope tags, inntags (innocuous tags), that they believe will have fewer ...expressed either without a tag or as multiple fusions with different N- and C-terminal tags. Additionally, by ...epitopes tags such as HA and MYC in vivo. The authors found that fusions to one particular tag, the IT6...expression of fusions between the POI and a TAP tag (a tag with two epitopes that can be used to sequentially...the POI-tag fusion from a plasmid or from a random genomic location than to express the tagged protein...two dimeric Broccoli tags (2xdBroccoli) to further enhance fluorescence of the tagged RNA. Bacterial and...

  19. Easing the Protein Purification Process with pCri

    Type
    Blog Post
    ...remove the tag post-purification. A sample of pCri constructs: pCri-1a, -4a and -9a. 6xHis tags enable affinity...of interest is purified using an affinity tag. Additional tags may be used to improve protein stability...researcher to test multiple tags for one protein quickly and easily. These tagged plasmids also include cleavage...remove these tags from the purified protein. For more detailed information on these and other tags used in ...integral-membrane protein constructs) protein tag. MISTIC drags tagged proteins into the membrane and may improve...which includes plasmids with different varieties of tags, both N- and C-terminal. Each set of plasmids uses...solubility and stability. TRX and signal peptide tags help proteins with disulfide bonds fold properly...

  20. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...strep-tag and, more recently, OLLAS (Escherichia coli OmpF linker and mouse langerin) and Sun Tag (Viswanathan...antibodies can have low affinity for these tags and even multimeric tags are frequently insufficient for detection...FP and epitope tags, researchers in Loren Looger’s lab have developed new molecular tags combining the ...antibody recognition of epitope tags. Thus they have created antigenic protein tags called “spaghetti monster...interest (POI), that facilitate immunohistochemistry (IHC) experiments with tag-specific antibodies. The most...Viswanathan, 2015). The principal advantage of epitope tags for IHC is the availability of reliable primary... are unavailable entirely. The other advantage is that these tags are small (8-12 amino acids) and neither...
Showing: 81 - 100 of 264 results