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We narrowed to 192 results for: lenti

Showing: 81 - 100 of 192 results

  1. Hot Plasmids - October 2020

    Type
    Blog Post
    ... The Human GlycoGene CRISPR Library is a new lentiviral CRISPR library that targets human glycosyltransferase...inhibition pooled library is now available as lentiviral preps in addition to the pooled library format...library inhibits over 18,000 human genes. Find the lentiviral preps at Addgene hSyn1-SIO-stGtACR1-FusionRed...

  3. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...MSCV-IRES-Luciferase IRES Retroviral pWPI IRES Lentiviral AmCyan-P2A-mCherry P2A Mammalian pC5Kan-P2A...-P2A P2A Insect pUltra P2A and T2A Lentiviral Ac5-STABLE2-neo T2A Insect   For the co-expression....) PubMed. 3. Highly Efficient Multicistronic Lentiviral Vectorswith Peptide 2A Sequences. Ibrahimi et...

  4. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    ...Brunello CRISPRko sgRNA library as pooled library or lentiviral prep! Dolcetto: human CRISPRi sgRNA library While...the Dolcetto library! Now also available as a lentiviral prep. Calabrese: human CRISPRa sgRNA library ...function. Find the Calabrese pooled library and lentiviral prep here! Applications for the Root and Doench...

  5. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type
    Blog Post
    ...millions of embryonic stem cells (ESCs) with a lentiviral vector, an attenuated retrovirus in the same ...same family as HIV. By design, our lentivirii are genetically programmed to CRISPR out the enhancers we identified...cells. Remove this pillar and the ESC dies. Order Lentivirus from Addgene If you’re interested in designing...

  7. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    ...depression. The researchers found that injecting lentiviral vectors expressing BDNF in the hippocampus causes...floating. In the same paper, the authors used lentivirus to introduce an shRNA to downregulate expression...Chandrasekar, and Jean-Luc Dreyer. "Selective lentiviral-mediated suppression of microRNA124a in the hippocampus...

  8. Hot Plasmids - November 2023

    Type
    Blog Post
    ...components to reprogram delivery vehicles like lentiviral vectors, CreVLPs, and eVLPs for in vivo and in... to target multiple delivery systems, such as lentivirus and MMLV gag, to specific cell types. It can ...cytoplasm and can be fluorescently labeled. Using lentiviral vectors to stably or inducibly express tagged...

  9. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ...people worldwide are currently infected with the lentivirus HIV-1. Today, HIV-1 can be controlled with antiviral... with HIV-1. For two HIV-1 strains, Cas9/gRNA lentiviral expression significantly reduced the HIV-1 copy...PubMed Central PMCID: PMC4778041. Find CRISPR and lentiviral packaging plasmids used in this publication at...

  10. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...Multiple Lentiviral Expression Systems (MuLE) Kit: This kit can be used to create lentiviral vectors expressing...CRISPR/Cas9-based genome engineering from a single lentiviral vector.” Nucleic Acids Research 42(19) (2014)...

  11. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    ...categories (e.g., for a lentiviral vector to be used in mammalian cells, check Lentiviral and Mammalian Expression...be prone to recombination in bacteria) such as lentiviral, retroviral, and AAV plasmids, we recommend the...

  12. Zhang Lab CRISPR Page

    Type
    Collection
    ...system - lentiCRISPR - sgRNA and SpCas9 together 2 vector system - lentiCas9-Blast and lentiGuide-Puro (...#61425 - #61427 are intended for lentivirus production : 61425 : lentiviral vector encoding dCAS9-VP64 with... top GeCKO Screening Library GeCKO are pooled lentiviral CRISPR libraries (both human and mouse) for performing...with 2A Blast resistance marker 61426 : lentiviral vector encoding the MS2-P65-HSF1 activator helper complex...complex with a 2A Hygro resistance marker 61427 : lentiviral sgRNA cloning backbone with MS2 loops at tetraloop...

  13. CRISPR Guide

    Type
    Collection
    ... libraries. What Are Pooled Lentiviral CRISPR Libraries? Pooled lentiviral CRISPR libraries are a heterogeneous...need to generate lentivirus containing the entire library (Figure 8D). This lentivirus will then be used...genome-wide screens using CRISPR involves pooled lentiviral CRISPR libraries. Figure 8: Overview of genome-wide...heterogeneous population of lentiviral transfer vectors. In most cases, each plasmid contains an individual...Figure 8A), then cloned in a pooled format into lentiviral transfer vectors (Figure 8B). Libraries come ...library is suitable for your experiment. Although lentiviral libraries are the most popular method for CRISPR...delivery to cells that are poorly transduced by lentivirus. Non-mammalian CRISPR libraries are also available...

  14. Plan Your Experiment

    Type
    Collection
    ...production, see our viral vector guides . Lentiviral vectors Lentiviral vectors are a popular option for producing...types like primary cells. For CRISPR delivery, lentiviral and adeno-associated virus (AAV) vectors are ...CRISPR components) integrates into the genome. Lentiviral vectors are a good option for difficult-to-transfect...we don’t discuss CRISPR screens in this guide, lentiviral vectors are a common choice for conducting genome-wide...CRISPR . Browse Addgene's collection of in-stock lentiviral preps . AAV vectors AAV vectors offer some advantages...advantages for viral vector delivery. Both lentiviral and AAV vectors are safe to use in the lab, but ...into your viral vector) is smaller than that of lentivirus. Thus, AAVs are generally only compatible with...

  15. Mammalian RNAi Tools

    Type
    Collection
    ...Addgene; browse by constitutive lentiviral shRNA, cre-regulated lentiviral shRNA, and more....Addgene viral protocols Lentiviral packaging plasmids Web Resources Trono Lab Lentivectors Toolbox Optimized...shRNAs are often delivered using viral vectors. Lentiviral vectors are a preferred method for long-term ...for cloning in shRNAs. Most plasmids are in a lentiviral backbone, with some retroviral and AAV backbone...

  16. Beginner's Guide to Viral Vectors

    Type
    Blog Post
    ...most important characteristics of retroviruses, lentiviruses, AAVs, and adenoviruses in this easy-to-use ...? Check out our viral vector guide pages, our Lentivirus FAQ, and our viral service pages. Click on the...

  19. An Addgene Summer Internship

    Type
    Blog Post
    ...weeks to work on cloning the Syntaxin3 gene in a pLenti vector backbone. During those five weeks, I worked...a 100% successful clone of the Syntaxin3 in the pLenti backbone! Once everything was confirmed, I worked...

  20. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    ...for inducible CRISPR inhibition (CRISPRi). A) Lentiviral vector design. B) Strategy for leak-free transcriptional...highlights from the past couple months: A new lentiviral CRISPR library from Alec Kimmelman targets mouse...
Showing: 81 - 100 of 192 results