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We narrowed to 191 results for: lenti

Showing: 81 - 100 of 191 results
  1. Addgene’s Viral Service - Why Virus? Why Now?

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    Blog Post
    ...small but growing catalog of ready-made AAV and Lentiviral preps. This new Viral Service represents Addgene...establish consistent, high-quality protocols for lentiviral production. Bryan Roth’s lab from the University...the Addgene Blog Check Out the Top Requested Lentivirus and AAV of 2016 Learn How You Can Use the Viral...
  2. dTAG - You're it!

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    Blog Post
    ...available from Addgene. These plasmids enable lentiviral expression of N- or C-terminally FKBP12F36V-tagged...unclear which terminus should be tagged, the dTAG lentiviral plasmids can serve as a rapid means of comparing...multiple FKBP12F36V-tagged proteins using our lentiviral expression platform, to assess selective degradation...
  3. Hot Plasmids - October 2020

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    Blog Post
    ... The Human GlycoGene CRISPR Library is a new lentiviral CRISPR library that targets human glycosyltransferase...inhibition pooled library is now available as lentiviral preps in addition to the pooled library format...library inhibits over 18,000 human genes. Find the lentiviral preps at Addgene hSyn1-SIO-stGtACR1-FusionRed...
  4. Plasmids 101: Multicistronic Vectors

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    Blog Post
    ...MSCV-IRES-Luciferase IRES Retroviral pWPI IRES Lentiviral AmCyan-P2A-mCherry P2A Mammalian pC5Kan-P2A...-P2A P2A Insect pUltra P2A and T2A Lentiviral Ac5-STABLE2-neo T2A Insect   For the co-expression....) PubMed. 3. Highly Efficient Multicistronic Lentiviral Vectorswith Peptide 2A Sequences. Ibrahimi et...
  5. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

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    Blog Post
    ...millions of embryonic stem cells (ESCs) with a lentiviral vector, an attenuated retrovirus in the same ...same family as HIV. By design, our lentivirii are genetically programmed to CRISPR out the enhancers we identified...cells. Remove this pillar and the ESC dies. Order Lentivirus from Addgene If you’re interested in designing...
  6. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

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    Blog Post
    ...Brunello CRISPRko sgRNA library as pooled library or lentiviral prep! Dolcetto: human CRISPRi sgRNA library While...the Dolcetto library! Now also available as a lentiviral prep. Calabrese: human CRISPRa sgRNA library ...function. Find the Calabrese pooled library and lentiviral prep here! Applications for the Root and Doench...
  7. Deciphering the Mysteries of Behavior with Viral Vectors

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    Blog Post
    ...depression. The researchers found that injecting lentiviral vectors expressing BDNF in the hippocampus causes...floating. In the same paper, the authors used lentivirus to introduce an shRNA to downregulate expression...Chandrasekar, and Jean-Luc Dreyer. "Selective lentiviral-mediated suppression of microRNA124a in the hippocampus...
  8. Hot Plasmids - November 2023

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    Blog Post
    ...components to reprogram delivery vehicles like lentiviral vectors, CreVLPs, and eVLPs for in vivo and in... to target multiple delivery systems, such as lentivirus and MMLV gag, to specific cell types. It can ...cytoplasm and can be fluorescently labeled. Using lentiviral vectors to stably or inducibly express tagged...
  9. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

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    Blog Post
    ...people worldwide are currently infected with the lentivirus HIV-1. Today, HIV-1 can be controlled with antiviral... with HIV-1. For two HIV-1 strains, Cas9/gRNA lentiviral expression significantly reduced the HIV-1 copy...PubMed Central PMCID: PMC4778041. Find CRISPR and lentiviral packaging plasmids used in this publication at...
  10. CRISPR References and Information

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    ...amplification 1 vector system: lentiCRISPR v2 2 vector system: lentiCas9-Blast and lentiGuide-Puro packaging plasmids...CRISPOR batch (Link opens in a new window) for lentiviral screens. sgRNA Scorer 2.0 (Link opens in a new...D10A (pX335) PDF, 255 KB Zhang sgRNA cloning lentiCRISPR v2 packaging plasmids: pVSVg , psPAX2 ; positive...
  11. Fluorescent Protein Guide: Empty Backbones

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    ...pmiRFP670-N1 - Mammalian Expression pLenti6.2_miRFP670 - Mammalian Lentiviral Expression pBAD/His-miRFP670 -...Prone to dimerization pLV-Azurite - Mammalian Lentiviral expression pB18cmBFPAzurite - Bacterial Expression...pCAG-GFP - Mammalian Expression pLV-eGFP - Mammalian Lentiviral Expression Ac5-STABLE1-neo - Insect Expression... Mammalian Expression pLV-mCherry - Mammalian Lentiviral Expression pCS2+8CmCherry - Zebrafish/Xenopus...smURFP 642 670 32 3.2 39 min Prone to dimerization pLenti-smURFP - Mammalian Expression Return to top Near-Infrared...
  12. Viral Vector Guides and Plasmids

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    ...Browse Guides and Plasmids Lentiviral Vector Guide | Lentiviral Plasmids Lentiviral vectors are versatile ...Infected Host Genome Integration Immune Response Lentiviral Stable RNA < 8 kb 80–130 nm Dividing/Non-dividing...
  13. CRISPR 101: Multiplex Expression of gRNAs

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    Blog Post
    ...Multiple Lentiviral Expression Systems (MuLE) Kit: This kit can be used to create lentiviral vectors expressing...CRISPR/Cas9-based genome engineering from a single lentiviral vector.” Nucleic Acids Research 42(19) (2014)...
  14. How to Deposit Your Plasmids with Addgene

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    Blog Post
    ...categories (e.g., for a lentiviral vector to be used in mammalian cells, check Lentiviral and Mammalian Expression...be prone to recombination in bacteria) such as lentiviral, retroviral, and AAV plasmids, we recommend the...
  15. Beginner's Guide to Viral Vectors

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    Blog Post
    ...most important characteristics of retroviruses, lentiviruses, AAVs, and adenoviruses in this easy-to-use ...? Check out our viral vector guide pages, our Lentivirus FAQ, and our viral service pages. Click on the...
Showing: 81 - 100 of 191 results