We narrowed to 565 results for: mme

...questions and comments go up early in the day, and the moderators were on top of any comments that were at...Anything”) on r/Science. You can see some of Joanne’s comments on the AMA process below, but we also wanted to... scientists who will find it useful, I highly recommend doing an AMA! Before I turn it over to Joanne,...resources. I’ve said this before, but I also highly recommend taking every opportunity you get to speak publically...

...protein expression and some of the most popular commercial strains designed to avoid them. Why do I need...gal dcm rne131 (DE3) General expression; not recommended for toxic proteins BL21-A1 Tetracycline Arabinose-inducible...expression plasmid from these strains is not recommended as these auxillary plasmids may be isolated during...interest from a T7 promoter on the plasmid. Many commercial strains carry the DE3 lysogen, as indicated by...favorite protein expression tips and tricks! Leave a comment below or browse Addgene’s collection of empty backbones...

...Pre and Post (Post recommended) UV Light 0.1 GelGreen Pre and Post (Post recommended) UV or Green (~500nm...important to digest plenty of starting material. We recommend 1.5-2μg of insert and 1μg of plasmid backbone....space to cut out the bands. Because of this we recommend that you use a wide gel comb, run the gel on the...covalent bonds are indicated in red).     We recommend around 100ng of total DNA in a standard ligation...

...antibodies and inhibitors of attachment and fusion. Recommended Reading For those looking to delve deeper or ...there are plenty of online resources available. I recommend a few: -The recently established Ebola Genome ...EBOV’s past, present, and future, don’t miss Carl Zimmer’s coverage on the subject in the New York Times ...resource to add, please tell us about it in the comments. ...

...workbenches such as Benchling (commercial, online), DESKGEN (commercial, online), or ProtospacerWB (academic...to other more suitable software such as ApE or commercial workbenches such as MacVector or Benchling. ...specific organisms. For this reason I generally recommend that laboratories that have already attempted ...first time, it is best to choose a tool based on immediate needs and re-evaluate after several experiments...

...microscopy techniques can be replicated with commercially available consumer-grade components. In this...account in the development process. Widely used, commercially available tools such as the Raspberry Pi, the...become an established alternative to high-priced commercial devices.     Fig. 2: Workflow for open...and assembly designs based around the use of commercially available tools and components. Rapid prototyping...

...However it’s difficult, especially as a newbie reprogrammer, to decide which method to use. This post provides...suited for these types of studies. What cells are reprogrammed will also affect the outcome, since not all ...research goals. Are you a new or experienced reprogrammer? Which methods do you have experience using?...Let us know about your iPSCs experiences in the comments below! References  1. Sano, Masayuki, Minoru ...PMID: 27764162. PubMed Central PMCID: 5072705. 2. Sommer, Cesar A., and Gustavo Mostoslavsky. "The evolving...

...current lead dTAG molecule is dTAG-13 due its immediate action, potent ligand performance, great depth... and in vivo utility. dTAG molecules will be commercially available in the near future, and we will provide...degradation in your context. Immunoblotting is recommended, while quantitative proteomics experiments will...  References Nabet et al. The dTAG system for immediate and target-specific protein degradation.. Nature...

...repeats separated by an 8-bp long asymmetric core spacer sequence. The asymmetry in the core sequence gives ...site have been created, which contain a unique asymmetric spacer "NNNTANNN", where "N" indicates which ... cre-dependent techniques?  Let us know in the comments! References 1. Bacteriophage P1 site-specific...

...into the AdV by recombination. It is therefore recommended to test for the presence of RCA in the viral ...cells . Alternatively, antibody-based assays are commercially available for faster detection of RCA. 2nd Gen... can be stored at -80C for later use or used immediately for amplification.*Pro-Tip*: The cells will become... concentration kits that do not use CsCl are commercially available. Titer calculation (1 or 10 days) ...

...changing flower project was inspired by a Facebook comment.  In our day job, we worked on plants engineered... to see that same anger. Instead, social media comments were more often along the lines of “I hate GMOs...thought we could be taught. They invested in us this summer – $30,000 for research and another $30,000 in mentorship...entrepreneurship, share your thoughts with us in the comments below. We’re happy to answer questions about this...

...sequence/gene - we set our threshold at >100 hits - immediately raises a red flag. For example, AAV-68544 contained... in a given viral prep. With this in mind, we recommend that users titrate their AAV vectors to find the...2015). This higher level of quality control is recommended but not yet required for clinical-grade materials... AAV vectors for your research. Please use the comments section below to let us know if you have any specific...

...cells when something does go awry. The process of programmed cell death, or apoptosis, is one such mechanism...directly inhibit the ability of a cell to undergo programmed cell death while simultaneously stimulating growth... potential therapeutic agents. Nevertheless, a glimmer of light remains for this elusive target. Recent...resource! Please leave us your thoughts in the comments section below. Michael G. Lemieux is a Scientist...

...simultaneously allowing scientists to actively comment, annotate, and edit protocols. After my introduction...them public for others to use, and add your own comments. Currently you can find protocols from NEB, various...protocols to share with others. The site allows you to comment on protocols and create lists of your favorite ...software app that I missed, let me know in the comments below and leave your thoughts on how the app works...

...to maximize the alignment length. While this recommendation is not infallible, we have found it saves analysis...sequencing result and can easily translate it, we recommend using blastp over blastx. The primary advantage...confirm a tag or fusion protein is in-frame, we recommend using blastx with the "Align two or more sequences... to confirm your plasmid sequencing results or comments on our suggestions? Share your thoughts here to...

...Polyclonal Can be labeled with multiple dyes Commercially available Not recombinant; Require animal... -mouse versions are currently available Not commercially available Small but Mighty: Advantages ...and several maleimide-conjugated dyes are also commercially available. References 1. Pleiner, T., Bates...supernatant is only $40, significantly cheaper than commercially available antibodies....

...highlight these recent advancements and offer commentary on their application to neuroscience research...To highlight this advantage, they selected the immediate-early gene Bdnf, which encodes brain-derived neurotrophic...early in response to neuronal activity (hence, “immediate-early”) and consequently regulates neuronal physiology...and Bdnf IV overexpression variants were other immediate-early genes associated with neuronal activity,...

...references organized from the beginning. I also recommend using the citation style of (Last name, Year) ...about having to hunt down sources later on.  A comment about writing: initially I felt that I needed a...get done between office hours and experiments. A comment about citing: I wasn’t sure if I should cite reviews... be revolutionary, but you could include a few comments on where you think the field is going or what ...

...cleavage. How does SHERLOCK work? Cas13 can be programmed with crRNA to target an ssRNA of interest, for...pathogen. Once Cas13 recognizes and binds to the programmed sequence, it can promiscuously cleave surrounding...cleavage byproducts. Thus, binding of Cas13 to the programmed region of interest would lead to both the cleavage... that a cleaved reporter could be detected on commercial lateral flow strips, similar to pregnancy tests...

...therefore differ from library to library. We strongly recommend following the protocols provided by the depositing...have a specific protocol for producing virus, I recommend that you follow that protocol. If not, our protocol...polyethyenimine transfection protocol. I also recommend searching PubMed for any publications associated...ddPCR) to quality control our viral preps, we recommend determining the functional (infectious) titer ...