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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 292 results for: nif

Showing: 81 - 100 of 292 results

  1. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    ...study,epigenetic changes were statistically significantly associated with reduced life-span, but no changes...controlled, the reporter consistently showed significantly different FRET levels between unmethylated and...

  2. Hot Plasmids: Summer 2025

    Type
    Blog Post
    ...biosensor for cAMP dynamics. The mGold2 variants significantly enhanced photostability — 25-fold over mVenus...Created with BioRender.com. Steven Jacobsen and Jennifer Doudna’s labs engineered the tobacco rattle virus...

  3. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ... milliseconds to acquire one image, thereby significantly reducing the time it takes to image through ...Similar to light microscopy, there have been significant advances in tissue clearing techniques during...

  7. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    ...ancestry, Sean and Aaron knew that their study had significant statistical limitations, and expected to find...Cas9 from cutting, both of which can introduce significant bias into the results. But the reference genome...

  9. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ... channel as the Cas13 reporter), they could significantly amplify the detection signal for a single target...but is undergoing validation studies.     Jennifer Tsang contributed to updating this article. References...

  10. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...expressing it with additional cofactors or using significantly truncated forms that limit its functionality...Both PILATeS (18 kDa) and STReTCh (11 kDa) are significantly smaller than FRET-based mechanosensors (~60 ...

  11. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    ... the Addgene repository can save researchers significant time and removes a variable from the lengthy ...trends in our own repository data, giving us significant insight into which vectors are likely to be of...

  12. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...Ward T.H., Margison G.P. “Mycoplasma infection significantly alters microarray gene expression profiles.”...Ellem K.A., Cleghorn G., West M.J., Wei MQ. “The significance of controlled conditions in lentiviral vector...

  14. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    ...are not exclusive to IF, but they can have a significant impact on the outcome of your IF experiment. ...antibodies you choose for your experiment will have a significant impact on the protocol you use and the ultimate...

  15. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    ...has spread to almost every part of the world manifesting as a full-fledged pandemic. Containing the spread...emission reader Approach: FnCas9 Editor Linked Uniform Detection Assay (FELUDA) is a technique that uses...

  17. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ...tdTomato), they produce a temperature sensor that significantly outperforms previous designs. B-gTEMP is excitable...optimal conditions). While B-gTEMP itself is a significant improvement over previous temperature indicators...

  18. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...mice, it is sex-independent and does not show significant differences in transduction efficiency between...brain. Both AAV9 and AAV9.452sub.LUNG1 have significantly higher off-target expression in the liver than...This post was written by Rachel Leeson, with significant contributions from Cynthia Arokiaraj. Addgene...

  19. A Control for All Seasons

    Type
    Blog Post
    ...results, the signal from your target needs to be significantly higher than the background noise (Waters 2009....14112. PubMed Central PMCID: PMC5773976. Waters, Jennifer C. “Accuracy and Precision in Quantitative Fluorescence...

  20. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...of interest. The piggyBac™ system allows for significantly enhanced random integration efficiencies that... If the cell’s chromatin structure undergoes significant rearrangement (such as when a stem cell initiates...
Showing: 81 - 100 of 292 results