...to ensuring plasmid quality is to stay organized. Here at Addgene, we collect data on every plasmid we ... Plasmid Learn How Addgene Uses Barcodes to Track Samples Resources on the Addgene Website View our ...the best things about sharing plasmids through Addgene is that we provide an added level of confidence...can take to verify your own plasmids. Staying organized With hundreds of samples coming through our doors...selection markers. You don’t need a fancy database to organize your own plasmid collection, although you will...physical sample and its associated information are at Addgene, we are ready to sequence verify the plasmids. ...plasmid you are working with, but, in most cases at Addgene, we check what we call the “insert,” (the gene ...

...tool to advance our knowledge of brain-visceral organ signaling. The problem  Previously, there were... for monitoring how both the brain and visceral organs interact during various behaviors. Either tissue...common neuroscience tools into the brains of model organisms, including the Anikeeva lab’s work on a similar...applications are to expand the tool to different brain-organ axes. Alternatively, the system could be used to...initiative plasmid collection More resources on the Addgene blog Plasmid tools for microbiome studies Channel....org/10.1038/s41587-023-01833-5 Resources on Addgene.org Optogenetics guide Chemogenetics guide BRAIN...

...application. Even within an organization, there can be variance. At Addgene, our content team, where writing...asks for strong organizational skills. You might think, “I’d be good at that! I organized my lab data so...explain why you are interested in working for that organization or group. This is particularly important for...title of the position] at [name of the company/organization]. I am a [adjective that describes you] scientist...updating this post, I took an informal poll of Addgene hiring managers. All of them stated that a good...that particular job, your commitment to the organization's mission, or your deep interest in the lab's... in May 2024.     Additional Resources on the Addgene Blog Learn to Develop Your Professional Network...

...also our mission here at Addgene! Along with other open-science organizations, we support scientists in...their CV or grant applications. A number of organizations (such as ASAPbio) advocate for preprints, and...manuscript to a journal for formal peer review. Some organizations, including some journals, also support open ...general public (say, policymakers or community organizations) to use your work but don’t think they’ll have...the manuscript prior to submission. Preprints at Addgene Preprints are all about sharing scientific knowledge...significantly sooner. Plus, authors can include the Addgene ID for their deposited plasmids in the final journal...10.1371/journal.pbio.3000116 More resources on the Addgene blog Summer SciComm Series: Modes of Communication...

...plants available through Addgene. In addition to the plasmids described above, Addgene carries several useful... ease of use and utility in a wide variety of organisms.  While much of current CRISPR research focuses... plant species, including commonly used model organisms like Arabidopsis and Medicago truncatula and several...universal functionality of the CRISPR system in most organisms, some plant-specific changes to CRISPR components... are available to academic researchers through Addgene. CRISPR components for plant genome engineering...experimental design principles developed in other model organisms (see our CRISPR guide for common CRISPR principles...sequence) is consistent between plants and other organisms, but the promoter used to drive gRNA expression...

...critical in the business world. Companies and organizations that avoid all conflict can fall into “groupthink...individuals involved, and also for the labs, organizations, or businesses as a whole. When I’m attempting... sharing gets more challenging the bigger an organization gets, and creating new avenues of communication...should be communicated is necessary in a large organization, because confirming that every person has read... a common source of conflict. For instance, at Addgene, there are two teams whose primary role is customer...attempting to resolve conflicts within a given organization, it can help tremendously to have as much context... solutions that benefit themselves and their organization. Resolving conflicts is great, but avoiding ...

... available in your lab or organization, and approvals from the organization overseeing your work. Good... on the Addgene blog Mouse Modeling Part 1 Mouse Modeling Part 2 Five Popular Model Organisms Additional...the peritoneal cavity, or the space surrounding organs in the abdomen. They are a popular choice for administering...and to avoid potential off-target pokes to nearby organs. Restraint: scruffing Location: peritoneal cavity...Additional resources on Addgene.org Genome Engineering Guide CRISPR Mammalian Expression plasmids Cre-Lox plasmids...

...your current organization but also important to build relationships outside your organization. The mentor...mentors all around you. This third article in the Addgene Blog Mentoring series will cover 2 of the 7 mentoring...what makes a good mentoring program. If your organization has a mentoring program, consider tapping into...upcoming mentoring post in which I describe how to organize a successful peer mentoring circle. Asking someone...

... the kits Addgene offers to kickstart these applications, no matter what your model organism is. MoClo...across organisms. When using the MK system, many of the parts can be used in a variety of organisms, saving...019-46171-2. Resources on Addgene.org Modular Cloning (MoClo) Guide Addgene’s eBook Collection More resources...MoClo kit recommendations for different modal organisms and applications.    Plant applications We...starter for your general plant cloning experiments. Addgene also has two additional MoClo plant kits: Plant...libraries and tools available for certain model organisms and backgrounds, MoClo can be invaluable for more... complex editing and screening experiments in organisms that don’t yet have these tools built out. While...

...10.1186/s12870-020-02388-2 Resources on Addgene.org Addgene’s Modular Cloning Guide EcoFlex MoClo Kit...modular cloning? Modular cloning, or MoClo, is an organization scheme for multi-part DNA assembly. Simply put...other non-Golden Gate cloning methods. MoClo’s organization schema MoClo can be broken down into three hierarchical...with characterized behaviors are available from Addgene and other sources; second, characterizing a part...many guides/parts delivered) in certain model organisms. In mammals, most CRISPR libraries are assembled...targeting constructs and delivering them to these organisms via another optimized platform. Gene Stacking ...MoClo Kit MoClo Plant Parts Kit Resources on the Addgene blog High Complexity Golden Gate Assembly with...

...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...research and to keep useful discoveries coming. At Addgene, we’re huge supporters of science communication...from Science in the News, a graduate student organization that works to promote science communication....non-scientists. These programs are all run and organized by graduate student researchers. That’s right,...Additional Resources on the Addgene Blog Check Out our Interview with Science Rapper Tom McFadden Learn about...

...Fluorescent reporters are not as well suited for imaging organs located deep in the body, like the gastrointestinal...the reporter and fail to pinpoint what tissue or organ this signal is coming from.   Acoustic reporter...where the bacteria localize in relation to internal organs. The limit of detection for arg1 expressing ECN...research? arg1 and arg2 plasmids are available from Addgene! References 1 Bourdeau, R.W., Lee-Gosselin, A....PubMed PMID: 29300010. Additional Resources on the Addgene Blog Learn more about in vivo imaging tools The...Acoustic reporter genes for noninvasive imaging of microorganisms in mammalian hosts. Nature, 553, 86-90. PubMed...fluorescent protein reporter gene Resources on Addgene.org Find plasmids for in vivo imaging with fluorescent...

...educating the public and organizing events. I contacted local professional organizations and nonprofits. I volunteered...Central Indiana Science Outreach, or CINSO. We organize fun science events for adults and professional...communication, editing, marketing, and website organization. My short experience with my blog helped immensely...establishments such as museums, professional organizations, and other nonprofits to gain support for CINSO...more people to quit the lab. Resources on the Addgene Blog Read Joanne Kamen's Blog Series on Mentoring...Browse All of Our Career Posts Resources on the Addgene.org Find Plasmids for Your Research Learn About ...

...Resources on the Addgene Blog Learn about Synthetic Biology with BioBuilder Use Addgene Videos to Learn...School Students Explore Careers at Addgene Resources on Addgene.org Find Yeast Expression Vectors Check... a multi-gene pathway to study and a reference organism where deletion strains are available. Then, staff...clone similar sequences from a less-well studied organism into overexpression plasmids. Students next transform...transform deletion strains from the reference organisms with the overexpression plasmids. Finally, students...Saccharomyces. cerevisiae, makes a great reference organism for the project because it is non-pathogenic, ...these plasmids, which have been deposited with Addgene, to successfully analyze Met protein complementation...

...in flies Resources on Addgene.org Find fluorescent protein plasmids at Addgene Read our Molecular Biology...specifically manipulate a cell type of interest in an organism, all cells of that type are affected. Previous...recombination outcome. Find SPARC plasmids at Addgene To more precisely control the amount of labeling...versatile and can be applied across cell types and organisms by replacing the transcription factor binding ...the PhiC31 recombinase can work in other model organisms including mouse and fish, SPARC is likely generalizable...s41592-018-0184-y Additional resources on the Addgene blog Learn about Gateway cloning Read about genome...

...the bench, I have worked for seven different organizations in a variety of science policy roles including...Big P policy from a post in non-governmental organizations, universities, professional societies, and industry...is non-governmental work happening at large organizations who have to develop policies for their communities...on a neglected disease area. Depending on the organization, these changes to policy could shift how society...your time in graduate school through outreach organizations and science blogs. Other avenues for skill development...development include leadership of extracurricular organizations, working in student government, and teaching...Twitter at @Steph_Guerra Additional resource on the Addgene blog: Browse our blog posts on science career ...

...for all ages. Organize your data and prepare publications or your dissertation Organize data that you...began working remotely recently? Most of us at Addgene began working from home last week to flatten the...new. We usually have yoga class twice a week at Addgene HQ, but now we’re sharing links to online yoga ...before bed will help you prepare for sleep. At Addgene, our mindfulness group is sticking with their Headspace...virtually connected with the people in your life. At Addgene, we’ve started virtual activities like team lunches...societies hosting virtual networking events. Download Addgene’s Science Career guide to learn more about career...begin working on these drafts and for a few model organisms, Micropublication will accept negative data or...

...  Check Out How Addgene Thinks About Developing Lab Management Software    Organizing and sharing your...share your lab protocols online Addgene Website: http://www.addgene.org/ If you’re working with plasmids...Recently I learned that Addgene’s pLKO.1 cloning protocol is viewed around 3,000 times each month. I ...protocols. Since we began sharing this protocol on the Addgene website in December 2006, the pLKO.1-TRC cloning... mobile) tools are available for scientists to organize and share their scientific protocols. Here are...plasmids and you decide to share them using Addgene’s repository, you can also share your associated protocols...gRNA_Cloning Vector (deposited by George Church’s lab). Addgene has also cataloged a variety of useful molecular...

... at Addgene.org Order Plasmids from Addgene! Find Free and Open Educational Resources at Addgene! ...official by registering BosLab inc. as a nonprofit organization in the state of Massachusetts. Because we’re...local companies and BosLab has grown into an organization consisting of over twenty members and volunteers...microbes. We’ve ordered a few yeast plasmids from Addgene and are eager to do our first yeast transformations...Angela Kaczmarczyk! Angela Kaczmarczyk is a co-organizer at BosLab, a community biotech laboratory in Somerville... @ZebraElement     Additional Resources on the Addgene Blog Experimenting in an Open Source Lab: from...

...an essential coenzyme that is required by all organisms for life. Biotin is only synthesized by plants...enzymes catalyze the transfer of carboxyl groups to organic acids to produce important cellular metabolites...post-translational protein modification. Most organisms have less than five enzymes in the entire proteome...for biotinylating only one lysine in the entire organism on the biotin carboxyl carrier protein (BCCP)....is unaffected by changes in pH, temperature or organic solvents. Uses of biotin in molecular biology Biotin... plasmids that contain both AviTag and BirA at Addgene! Oligonucleotide labeling Biotin can be added to...PubMed PMID: 8245422. Additional resources on the Addgene blog In vivo biotinylation of bacterial fusion...