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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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In Category

We narrowed to 1,019 results for: ple

Showing: 81 - 100 of 1019 results

  1. E11 Bio PRISM Collection

    Type
    Collection
    ...subsets of barcode proteins to label and detect multiple injection sites in the same tissue, as well as...generates unique combinations of protein bits. B) Multiplex imaging of barcodes with expansion microscopy ...

  2. Viral Vector Guides and Plasmids

    Type
    Collection
    ...and are suited for specific research goals. For example, AAV vectors are typically preferred for in vivo... self-inactivating, meaning that they can only complete one round of transcription of their viral genome...

  3. Introducing an All-in-One CRISPR/Cas9 Vector System for Multiplex Genome Engineering

    Type
    Blog Post
    ...construction system for multiplex genome engineering. Image from Sakuma at al. (2015). Multiplex genome engineering... construction system for CRISPR/Cas9-mediated multiplex genome engineering is now available thanks to ...new tool in Scientific Reports in June. “The multiplexity is one of the most advantageous properties of...established way of making an all-in-one vector for multiplex genome engineering.” Now, they’ve filled that...Cas9-nickase vectors work successfully to target multiple sites in cultured human cells. While it has not...be applied to efficient viral vector-mediated multiplex genome engineering. The system now provides an...an efficient targeting strategy for multiplex editing of genomes. It will also allow simultaneous activation...

  4. Chemogenetics AAV Preps

    Type
    Collection
    ...available items found to match all requested criteria. Please consider using fewer filtering options. *AAVrg ...would like to see available in viral prep format. Please note this does not guarantee viral vector packaging...

  5. Worm Expression Resources

    Type
    Collection
    ...below and not included in this table. For a more complete list of plasmids that can be used in worms, search...backbones, and constructs for endogenous tagging. Some examples of useful worm CRISPR resources, along with links...

  6. AAV Packaged on Request

    Type
    Collection
    ...transfer plasmid, including legal approval and an implementation letter DNA amplification Viral vector production...researchers with troubleshooting and results that complement published data, capturing the insights that often...

  7. p53 Pathway

    Type
    Collection
    ...for the gene, or browse the gene list below. For multiple isoforms or subunits, individual links to each...available plasmids for the gene. For components with multiple isoforms or subunits, individual links to each...

  8. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

    Type
    Blog Post
    ... to implement in practice because FRET depends on a number of hard-to-achieve factors. For example, FRET...Bimolecular Fluorescence Complementation (BiFC). What is bimolecular fluorescence complementation? Bimolecular fluorescence... TK (2006) Design and implementation of bimolecular fluorescence complementation (BiFC) assays for the...often easier said than done. Fortunately, a complementary method exists for visualizing protein-protein... fluorescence complementation is a method for detecting protein-protein interactions that uses split fluorescent...fragments that individually lack fluorescence. When complementary FP fragments are fused to two hypothesized protein...interaction partners (PIPs), the FP fragments form the complete FP because the PIPs they’re tethered to bring ...

  9. Fluorescent Proteins: FRET

    Type
    Collection
    ...Y67C) constructs for measuring energy transfer to multiple acceptors Koushik et al. (2009) Anomalous surplus...surplus energy transfer observed with multiple FRET acceptors mTurquoise2-mVenus FRET calibration standards...

  10. Control AAV Preps

    Type
    Collection
    ...available items found to match all requested criteria. Please consider using fewer filtering options. Browse ...would like to see available in viral prep format. Please note this does not guarantee viral vector packaging...

  11. Optogenetics AAV Preps

    Type
    Collection
    ...available items found to match all requested criteria. Please consider using fewer filtering options. Browse ...would like to see available in viral prep format. Please note this does not guarantee viral vector packaging...

  12. Retrograde AAV viral preps

    Type
    Collection
    ...available items found to match all requested criteria. Please consider using fewer filtering options. Browse ...would like to see available in viral prep format. Please note this does not guarantee viral vector packaging...

  13. Plasmids for Stem Cell Research

    Type
    Collection
    ...or somatic) stem cells, can differentiate into multiple cell types, but are typically limited to related...generate iPSCs. The generation of iPSCs is relatively simple in concept: ectopically express a cocktail of stem...

  14. Biosensor AAV Preps

    Type
    Collection
    ...available items found to match all requested criteria. Please consider using fewer filtering options. *AAVrg ...would like to see available in viral prep format. Please note this does not guarantee viral vector packaging...

  16. Synthetic Biology - Metabolism

    Type
    Collection
    ... related to metabolic pathways and components. Examples Include: Biofuels Natural Product Pathways Efflux...

  19. MAPK Plasmids

    Type
    Collection
    ...programs by acting as an integration point for multiple biochemical signals. In collaboration with numerous...
Showing: 81 - 100 of 1019 results