Antibodies 101: Beyond Surface Labeling
Type
Blog Post
...system, as is commonly used in western blotting or ELISAs.
A popular method for indirect labeling employs...
We narrowed to 91 results for: sas;
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How to Negotiate a Successful Lab Start Up Budget
TypeBlog Post... institute is located. For example, salaries in Kansas versus salaries in NYC will vary widely. Therefore... -
How to Design Your gRNA for CRISPR Genome Editing
TypeBlog Post...editing with CRISPR–Cas nucleases, base editors, transposases and prime editors. Nat Biotechnol 38:824–844... -
Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility
TypeBlog Post...Last Wednesday we worked with the Harvard GSAS Science Policy Group to organize a Minisymposium on Reproducibility... -
Antibodies 101: Antibody Engineering and Directed Evolution
TypeBlog Post...support common research tools like western blots, ELISAs, immunoprecipitation, and flow cytometry. The better... -
Sequencing Options for CRISPR Genotyping
TypeBlog Post...research setting, but these events could have disastrous health effects in the clinic. NGS-based methods... -
With an Eye Towards the Future, We Look Back at the March for Science
TypeBlog Post...funding are critical to averting a potential climate disaster. However, policy makers use these two fingers ... -
Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2: Reprogramming and Transdifferentiation
TypeBlog Post...5): p. 1174-1174 e1. PubMed PMID: 22632979. 14. Sasaki, K., et al., Robust In Vitro Induction of Human... -
Antibody Guide
TypeGuide...two most common types of ELISAs are direct or sandwich ELISAs. For direct ELISAs, an antigen or protein ...differentiate between proteins. HRP is also used in ELISAs, which only measure a single antibody signal. Here...Quantification methods, such as western blots or ELISAs, are used to quantify relative amounts of protein...standard curves to quantify exact amounts of proteins. ELISAs quantify a single protein per assay and typically...verified to contain your protein of interest (in ELISAs, the standard curve can serve as the positive control... -
27 Hot Plasmids from 2016
TypeBlog Post...VPR expression plasmid that uses the piggybac transposase to integrate dCas9-VPR into the genome of target... -
Immunology Research Plasmids and Resources
TypeCollection...glucose phosphate isomerase AMF, GNPI, NLK, PGI, PHI, SA-36 GPR17 G protein-coupled receptor 17 DKFZp686M18273...