We narrowed to 1,054 results for: tra

... interfere with transcription elongation. CRISPR Activation : Target the transcription start site. CRISPRa...fused to transcriptional activators that need to be within a given range of the transcription start site... perform by chemical transfection or electroporation. Expression can be transient, or you can generate...efficiently transfect. However, note that this method is most useful for cell lines that you can transfect at ...into cells. These methods are more transient than plasmid transfection or viral vectors, reducing the risk...your target cells are not transcribing DNA to mRNA, nor do they need to translate mRNA to protein. Instead...is helpful in cells that are difficult to transfect, transduce, or may not express common promoters. Browse...

...divided among multiple plasmids: Transfer plasmid — contains transgene, sgRNA, or shRNA of interest flanked...expression, multicistronic design enables tracking of viral transduction. See plasmid 46948 for additional variant...knockdown, multicistronic design enables tracking of viral transduction. See Meerbrey et al. (2011) for additional...20342 FUW-M2rtTA 3rd Contains reverse tetracycline transactivator. Rudolf Jaenisch 131626 pHAGE-mt-mKeima...envelope, and transfer plasmids Viral... Viral Plasmids Lentiviral Plasmids Lentiviral Plasmids Transfer Plasmids Packaging...types of viruses used in research. Lentivirus can transduce both dividing and non-dividing cells without a...

...so cells must be lysed or the substrate must be delivered intracellularly to measure luminescence. Gaussia...emission, substrate use, stability, or other features. AkaLuc : Engineered to use the substrate Akalumine...Bioluminescence Resonance Energy Transfer (BRET) Similar to FRET, energy transfer from the luciferase reaction...can be used to study processes beyond transcription and translation. Splicing reporters from Younis et al...insects. Luciferase catalyzes the oxidation of a substrate, leading to the emission of photons. Its robust...Regulatory elements such as promoters, enhancers and untranslated regions, or shRNA or CRISPR target sites can..., and different luciferases require different substrates and detection wavelengths. Firefly luciferase...

...containing transgene under CMV promoter Vogelstein 16404 pAdTrack For production of GFP-trackable viruses...containing transgene under CMV promoter Vogelstein 50957 RedTrackCMV For production of mRFP-trackable viruses...top Adenoviral Strains Browse bacterial strains for adenoviral vector production. ID Strain Description ...plasmids: A shuttle plasmid (or transfer plasmid) containing the transgene of interest, the ITRs, and homology...viruses containing transgene under a chosen promoter Vogelstein 16405 pAdTrack-CMV For production of GFP-trackable...plasmids, adenoviral backbone plasmids, and bacterial strains for adenoviral vector production in Addgene's repository...Shuttle Plasmids Adenoviral Plasmids Adenoviral Strains Adenoviral vectors are widely used for gene delivery...

...Adenoviral High transduction efficiency, but do NOT integrate into host genome pAdTrack-CMV - Shuttle...production AdEasier®-1 cells (strain) - Bacterial strain that contains AdEasy®-...miniTurbo in the mammalian nucleus pSF3-ultraID - Expresses ultraID in mammalian cells with the Tet-On or...expression Find yeast transformation plasmids with G418 selection Find nematode transgenesis plasmids with Neomycin...Backbones Mammalian CMV, SV40, EF1a, CAG, Ubc Transient expression - Gradia Lab plasmids for mammalian...Find more Tet-inducible empty backbones on our Tetracycline (Tet) Inducible Expression page Bacteria Lac...gene with N-terminal GST tag and TEV cleavage Transient expression - Gradia Lab plasmids for bacterial...

...pOpen-EcoRIM M.EcoRI DNA methyltransferase 165517 pOpen-Eco31IA Eco31IA DNA methyltransferase 165518 pOpen-NotIM...pOpen-NotIM M.NotI DNA methyltransferase 165527 pOpen-Eco31IB Eco31IB DNA methyltransferase 165536 pOpen-PstIM...pOpen-PstIM M.PstI DNA methyltransferase 165538 pOpen-SfiIM M.SfiI 165538 DNA methyltransferase 165547 pOpen-SapIM1...pOpen-SapIM1 M1.SapI DNA methyltransferase 165557 pOpen-SapIM2 M2.SapI DNA methyltransferase 165558 pOpen-EcoRVM...pOpen-EcoRVM M.EcoRV DNA methyltransferase 165577 pOpen-XbaIM M.XbaI DNA methyltransferase 165578 pOpen-NcoIM ...32 single-stranded DNA-binding protein 165549 pOpen-ETSSB Extreme Thermostable Single-Stranded DNA-Binding...Polymerases Restriction Endonucleases Reverse Transcriptases RNA Polymerases Other Enzymes Additional Resources...

...Overexpression Tracers Tetracycline Transactivators and Inducible Tools These AAV encode tetracycline-inducible...tools/controls and tetracycline transactivators that can be used with tetracycline (tet)-inducible expression...service encoding tet-off transactivators and tools for affinity purification (TRAP). Viral...Vector Packaging Service: Molecular Tools Tetracycline Transactivators Affinity Purification Neurophysiology...promoter. 8 Alexander Dityatev Anterograde Transsynaptic Tracers These AAV encode tools that can be used ...pAAV-ATLASsnCre Syn-driven, constitutive Anterograde transsynaptic tracer protein to express in presynaptic glutamatergic...pAAV-DIO-ATLASsnFlp Syn-driven, Cre-dependent Anterograde transsynaptic tracer protein to express in presynaptic glutamatergic...

...extensive selection of plasmids using tetracycline-regulated transcriptional activation or repression. The BIOFAB...generally necessary to purify it. Specific bacterial strains such as E. coli BL21(DE3) are used as biofactories...proteins in high yields. Proteins can then be extracted from the culture and purified through affinity...bacterial protein contamination and facilitates extraction. Browse our most popular plasmids for protein...MoClo) for use in E. coli . Addgene Blog E. Coli Strains for Protein Expression Protein Expression Epitope... Protein Interactions Förster resonance energy transfer (FRET) and bimolecular fluorescence complementation...that when a donor fluorophore is excited, it can transfer its energy to an acceptor fluorophore if they ...

...acids (ncAAs). This strategy, called genetic code expansion (GCE), has transformed synthetic biology by... Mehl 85496 pDule-Tet2.0 Tetrazine2.0 tRNA synthetase M. jannaschii Tetrazine 2.0 Bacterial TAG Ryan Mehl...Mehl 85497 pDule2-Tet2.0 Tetrazine2.0 tRNA synthetas M. jannaschii Tetrazine 2.0 Bacterial TAG Ryan Mehl...barkeri Tetrazine 3.0 Bacterial TAG Ryan Mehl 174081 pAcBac1-NES-Flag-R2-84-MbRS Tetrazine3.0 NES-Flag-R2...Vector Type PI Return to top Bacterial Strains Browse bacterial strains that have been modified to enhance...synthetase plasmids, target plasmids, and bacterial strains useful for expanding the genetic code in bacteria... Synthetase Plasmids Target Plasmids Bacterial Strains The genetic code for all life is based upon four...

...special trans-activating crRNA (tracrRNA) with homology to the short palindromic repeat. The tracrRNA helps... double stranded DNA target and cleaves both strands near the PAM. (6) The crRNA:tracrRNA:Cas9 complex...: dCas9 fused to transcriptional activators or repressors can modulate transcription in a reversible manner...have long utilized CRISPR as a powerful defensive strategy against viral invaders, and this system is proving...palindromic repeat sequences. (1) The CRISPR array is transcribed to make the pre-CRISPR RNA (pre-crRNA). (2) The...together separate the individual crRNAs. (3) The tracrRNA and Cas9 nuclease form a complex with each individual...individual, unique crRNA. (4) Each crRNA:tracrRNA:Cas9 complex seeks out the DNA sequence complementary...

...reprogramming factors Efficient germ-line transmission obtained with transgene-free induced pluripotent stem cells...Reprogramming Plasmids Differentiation and Transdifferentiation Plasmids Stem Cell Research Plasmids Stem...methods with the goal of helping readers choose a strategy suited to their research. Once you know which ...differentiated somatic cell into another via transdifferentiation. This method of cell lineage conversion ...technology. Browse Addgene's collection of Transdifferentiation Plasmids below. Many of the techniques for... for reprogramming, differentiation, and transdifferentiation utilize plasmids to increase expression ...human Klf4, Oct4, c-Myc, and Sox2 as VP-16 transcriptional activating fusions in single lentiviral vectors...

...Rinehart lab developed a new strain of recoded E.coli , rEcoli XpS (Bacterial strain #192872) , and an optimized... protein. Using the recoded strain of E. coli , C321.ΔA (Bacterial strain #68306) , with the optimized...phosphoserine orthogonal translation system, SepOTSλ (Plasmid #68292) , they were able to demonstrate unambiguous ...pLtetO MBP-MEK1 XX Amp) Strain 52055 EcAR7 Plasmid 52054 SepOTSµ ( aka. B40 OTS) Strain 34929 BL21ΔserB Plasmid...phosphorylation is one of the most abundant forms of posttranslational modification in cells and research into its...Lajoie et al., 2013) and improving the orthogonal translation system to make phosphorylated proteins with one...optimized phosphoserine orthogonal translation system, pSerOTS-C1* (V70) (Plasmid #188537) . These reagents...

...packaging capacity, high transduction efficiency, and high but transient transgene expression.They can induce...130 nm Dividing Yes Moderate AAV Transient or Stable* Single-stranded linear DNA ∼4.5 kb 18–26 nm Dividing.../Non-dividing No* Very Low Adenoviral Transient Double-stranded linear DNA 5–36 kb 105 nm Dividing/Non-dividing...of viral vectors and find viral production and transfer plasmids for a wide array of research purposes...are versatile tools because of their ability to transduce dividing and non-dividing cells. These virusal...immunogencity. These viral vectors can direct long-term transgene expression, but generally don't integrate into...just retroviral vectors, have a relatively high transduction efficiency and only infect dividing cells. They...

...Tips for Arabidopsis Transformation Save Time with Transient Plant Leaf Transformations Engineering the Plant...Figshare Plant Illustrations (Link opens in a new window) — Collection of illustrations and pictures of...System Plasmids for rapidly assembling plant transformation constructs, based on the Golden Gate method...contains yeast two-hybrid and bacterium-to-plant translocation vectors. Partially compatible with GoldenBraid...set of functional parts for assembling plant transformation vectors, compatible with MoClo. Golden Gate...Daniel Voytas 1000000236 MoClo Plant Parts III: Transformation & Genome Engineering Kit Collection of MoClo...MoClo Level 0 parts for plant transformation, including regulatory elements, coding sequences, basic CRISPR...

...sites are on separate DNA molecules (in trans), a translocation event is generated between the target sites...can also be useful for lineage tracing, evaluating infection/transfection efficiency, selecting desired...mycobacteriophage, are serine SSRs that cleave all four DNA strands at once. They can mediate the integration of a ...VCre, and Vika. These systems work by sequential strand exchange at a specific target site (called loxP...after recombination (inversion, deletion, or translocation) depends on the orientation of each target site... If the target sites (loxP) are on the same DNA strand in opposite directions, recombination results in...reversed. B) If the target sites are on the same DNA strand (in cis) in the same direction, the sequence between...

...into multiple plasmids for safety: Transfer plasmid — contains transgene, sgRNA, or shRNA of interest flanked... the transgene fused to eGFP. Hans Clevers 18760 MSCV IRES Luciferase MSCV Plasmid for transgene expression...and transfer plasmids. Viral... Viral Plasmids Gamma-Retroviral Plasmids Gamma-Retroviral Plasmids Transfer Plasmids...encoded on RNA. The genome includes a reverse transcriptase and an integrase to insert the viral genome ...can take advantage of this to stably integrate transgenes of interest into a variety of cell types. Wild-type...collection highlights popular gamma-retroviral transfer, packaging, and envelope plasmids. Read our Gamma-retroviral...

...- Tatjana Sauka-Spengler Lab Lineage Tracing Cell lineage tracing in multicellular systems typically relies...Lewis Lab PhOTO Zebrafish: A Transgenic Resource for In Vivo Lineage Tracing during Development and Regeneration...Periklis Pantazis Lab Multibow: digital spectral barcodes for cell tracing - Sean Megason Lab Zebrabow - Albert... (~3 months), ease of care and breeding, and transparency of the embryo and larvae, it is easy to culture...standard toolbox of genetic methods, such as Tol2 transposons, Cre-mediated site-specific recombination, morpholinos...emerged as a model system for human disease and translational research, including the study of cancer, inflammation...-Cas9 system - Shuo Lin Lab Re-purposed Ac/Ds transgenic system enabling CRISPR/dCas9-interference (CRISPRi...

...diluting or concentrating to a final concentration of 0.9–1.1 mg/mL. Antibody concentration is confirmed...Blotting (WB) Proteins are extracted from cells, separated by SDS-PAGE, transferred to a membrane, and labeled...of co-purified plasmid from transfection. This residual plasmid is extracted and amplified with primers...expression vectors, human embryonic kidney cells are transfected with the plasmid(s) encoding the heavy and light... the buffer can be removed by dialysis or gel filtration. Titer and Purity Antibodies are initially titered...serial dilutions of a standard antibody of known concentration and stained with Coomassie blue. Protein band...standard curve is generated. The final antibody concentration is calculated from the standard curve. Using...

...red over time, allowing tracking of processes such as intracellular trafficking or cell cycle dynamics....are monomers at low concentration but are prone to dimerization at high concentration. Proteins that are...eqFP611 559 611 35 Tetramer eqFP611-N1 - Mammalian Expression DsRed2 563 582 24 Tetramer pCAG-DsRed2 - Mammalian... - Mammalian Expression mStrawberry 574 596 26 4.5 50 min Monomer mStrawberry-N1 - Mammalian Expression...contain the A206K mutation are monomeric at all concentrations, so this is mutation is noted when present....CYPet-C1 - Mammalian Expression AmCyan1 453 486 11 Tetramer AmCyan1-N1 - Mammalian Expression mTFP1 462 492...Expression (cysteine-free SGFP2) ZsGreen 493 505 39 Tetramer pHIV-Zsgreen - Mammalian Expression (this is a...

...of Pennsylvania Vector Core. Transfections are performed using the transfer plasmid, a plasmid encoding...are purified by iodixanol gradient ultracentrifugation and concentrated to purity and titers adequate for...cell cultures are inspected for sterility. Transducibility Some viral vectors are tested in vitro and ...section for images). Figure 2: AAV Pro cells were transduced with either pAAV-Ef1a-mCherry-IRES-Cre (55634...cleared by low-speed centrifugation and then by filtration through a 0.45 µm polyethersulfone (PES) membrane... been stored at -80 °C and thawed. Lentivirus titration is performed using a 293T cell line in the presence...using a lentiviral droplet digital PCR (ddPCR) titration protocol (modified from Wang et al. (2018) (Link...