We narrowed to 145 results for: transfection
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TypeBlog Post...to modify it to improve yields. For example, transfecting the producer cell line with an expression vector...
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AAV Q&A with Tim Miles
TypeBlog Post...Animal Models Q9: Is there any difference in transfection efficiency when delivering these viruses via... -
Building and Validating Recombinant Antibodies: A Case Study
TypeBlog Post...IF-ICC.) This method was chosen because cells transfected with a plasmid to express the target antigen ... -
Kiran Musunuru on the Newest TALEN Genome-Editing System
TypeBlog Post...cells after the plasmids have been introduced by transfection or electroporation. All you do to create a protein... -
Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2: Reprogramming and Transdifferentiation
TypeBlog Post...plasmid electroporation (episomal) [7, 8] and mRNA transfection [9]. Many of the plasmids used for these methods...Weintraub, and A.B. Lassar, Expression of a single transfected cDNA converts fibroblasts to myoblasts. Cell,... -
Viral Vectors
TypeCollection...the delivery of genetic information to hard-to-transfect mammalian cells. In addition, "viral pseudotyping... -
A Practical Approach to Choosing the B(right)est Fluorescent Protein
TypeBlog Post...When a dish of mammalian cells is transiently transfected with a plasmid containing a fluorescent protein... -
Screening for Successful Genome Editing with Digital PCR
TypeBlog Post...PubMed PMID: 26914317. 4. Mock, U. et al. mRNA transfection of a novel TAL effector nuclease (TALEN) facilitates... -
Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME
TypeBlog Post...an unregulated fashion and thus a marker for transfection. The other (mKate2) is regulated in the 3’ UTR... -
Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins
TypeBlog Post...PPI) studies in plants that rely on transient transfection systems using Agrobacterium (such as Nicotiana... -
15 Years of Addgene: The Top 15 Plasmids
TypeBlog Post...pSpCas9(BB)). The 2A-GFP allows for screening of transfected cells. Find pSpCas9(BB)-2A-GFP (PX458). lentiCRISPR... -
Viral Vectors 101: Viruses as Biological Tools
TypeBlog Post...of cell types that are typically ‘difficult to transfect’, such as primary cells. They can also be directly... -
Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics
TypeBlog Post...base editor is split into two plasmids and co-transfected into cells for reconstitution. Image from Levy... -
Working with Nuclear Receptors
TypeBlog Post...laboratories. The development of the transient transfection platform for assaying transcriptional activity... -
Viral Production
TypeCollection... Vector Core (Link opens in a new window) . Transfections are performed using the transfer plasmid, a ... -
27 Hot Plasmids from 2016
TypeBlog Post...single gene. The gRNA expressing cells were then transfected with a Cas9-expressing plasmid to generate a ...luciferase. The bait and prey plasmids are co-transfected and proteins are immunoprecipitated from the ... plasmid is also available). Second, they co-transfected short-arm donor vectors containing mAID and either...efficiently creating stable cell lines using direct transfection precluding the need for virus. Furthermore, ... -
RaPID Detection of RNA-protein Interactions
TypeBlog Post... include both scrambled RNA motifs and cells transfected with BirA* protein alone (i.e. no RNA bait). ... -
Antibodies 101: Affinity Reagents
TypeBlog Post...bonds, monobodies can be easily expressed in transfected eukaryotic cells, allowing for them to be used... -
Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards
TypeBlog Post...hippocampal cultured neurons, imaged 14 days post-transfection, excited at 505 nm (right). Scalebar represents... -
Viral Vectors 101: Virus Safety
TypeBlog Post... plasmid). Viral production then proceeds by transfection of all three plasmids into a producer cell line...