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We narrowed to 144 results for: transfection

Showing: 81 - 100 of 144 results

  11. Working with Nuclear Receptors

    Type
    Blog Post
    ...laboratories. The development of the transient transfection platform for assaying transcriptional activity...

  12. Viral Vectors

    Type
    Collection
    ...the delivery of genetic information to hard-to-transfect mammalian cells. In addition, "viral pseudotyping...

  13. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...single gene. The gRNA expressing cells were then transfected with a Cas9-expressing plasmid to generate a ...luciferase. The bait and prey plasmids are co-transfected and proteins are immunoprecipitated from the ... plasmid is also available). Second, they co-transfected short-arm donor vectors containing mAID and either...efficiently creating stable cell lines using direct transfection precluding the need for virus. Furthermore, ...

  17. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    ... plasmid). Viral production then proceeds by transfection of all three plasmids into a producer cell line...

  19. Viral Production

    Type
    Collection
    ...the University of Pennsylvania Vector Core. Transfections are performed using the transfer plasmid, a ...

  20. AAV Production in HEK293 Cells

    Type
    Protocol
    ...cells in CS2 Day 2: Seed cells in CS5 Day 3 (am): Transfect cells Day 7 (am): Harvest cells Equipment Class...brands and lots of FBS can promote or inhibit transfection. Test a variety of brands and lots of FBS to...Polyethylenimine (PEI) 25 kDa MW Pro-Tip Other transfection reagents may be used in this protocol, but their...their conditions must be optimized. Plasmids for transfection: pHelper; pRC (Rep-Cap), plasmid expressing ... Pass and plate the cells the day before the transfection. Thaw a new vial of cells after 30 passages....to the incubator for 24 h–36 h. Proceed with transfection: Calculate the amount of each plasmid needed...Note that longer incubation times can reduce transfection efficiency. Add the OptiMEM + DNA + PEI solution...
Showing: 81 - 100 of 144 results