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We narrowed to 333 results for: uros

Showing: 81 - 100 of 333 results

  1. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

    Type
    Blog Post
    ...stimulation is a powerful tool used by neuroscientists to interrogate neuronal networks. A major drawback of this... method is the stray excitation of neighboring neurons in opsin dense regions. To overcome this issue,... C-terminus of the protein. Unlike the diffuse neuronal expression observed with wild type CoChR, soCoChR...describe a novel system for the precise mapping of neuronal networks at the single-cell level. Various soCoChR...available at Addgene. Shemesh OA, et. al. Nature Neuroscience. 2017. PubMed PMID: 29184208.        Synthetic...

  2. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    ...across the membrane of cardiac cells. Similarly, neuronal signaling is carried out by action potentials ...perceived signals to physical actions. Understanding neuronal pathways and the function of certain cells depends...be directed to specific types of cells, such as neuron subtypes, thanks to viral serotype specificity ...available VSDs has been successful even at the single neuron level in vitro. In vivo monitoring has also been...voltage is particularly physiologically relevant in neurons, myocytes, and endocrine cells. It follows that...voltage indicators advance their applications in neuroscience. Curr Opin Biomed Eng. 2019. 12:111-117. doi...

  3. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...Lee and Pantelis Tsoulfas of the Department of Neurological Surgery at the University of Miami. The beginning...light microscopy, particularly related to the neurosciences.  These developments in microscopy coupled with...of transgenic mice in which a subpopulation of neurons are brightly labeled with GFP (e.g. Thy1-YFP-H ...LSFM with multiple tissue clearing strategies and neuronal labeling methods will greatly aid our understanding...331–336. Pubmed. P. Keller, M.B. Ahrens.  (2015) Neuron, 85, 462-483. Pubmed. E.H.K. Stelzer. (2015). Nature...H. Lee, A. Dawood, M. Carballosa, et al, (2015) eNeuro, March/April 2015, 2(2) e0001-15.2015. Pubmed. ...

  4. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    ...could identify the ancestry of the cell lines (European, East Asian, Indigenous American, Africa, or South...lines used in DepMap CRISPR screens are of either European or East Asian ancestry, Sean and Aaron knew that...dependencies that were clearly associated with European or East Asian ancestry groups, far more than expected...human population. Rather, it primarily reflects European ancestry.  Understanding the impact Fig...to better understand how the use of a primarily European reference genome could affect their analysis. ... by the Cancer Dependency Map) than lines from European or East Asian ancestries. These SNPs, however,...

  5. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

    Type
    Blog Post
    ...unable to reach all regions of the CNS. PHP.B, a neurotropic variant of AAV9, is better than AAV9 at gaining...receptor used by PHP.B viruses. Creating PHP.B, a neurotropic AAV9 variant PHP.B’s specificity for C57BL/6J... and transduces the majority of astrocytes and neurons across multiple CNS regions. PHP.eB, an enhanced...PMID: 30819613. Hordeaux, Juliette, et al. "The neurotropic properties of AAV-PHP. B are limited to C57BL...transduction efficiency in the marmoset brain." Neuroscience letters 665 (2018): 182-188. PubMed PMID: 29175632...central and peripheral nervous systems." Nature neuroscience 20.8 (2017): 1172. PubMed PMID: 28671695. PubMed...

  6. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ...various neurotransmitters, neuropeptides and other ligands - almost A to Z! - from ATP to urotensin. The ...catalog is a new class of sensors for neuropeptides and neurotransmitters. Motivated to develop a biosensor...MTRIA sensors will be useful for investigating neuronal signaling and for engineering improved sensors...

  7. Let There Be LITE Plasmids

    Type
    Blog Post
    ...When neuroscience graduate student Silvana Konermann first entered Feng Zhang’s lab at MIT, the use of...now available in Addgene’s repository for use in neurons, both in culture and in living brain tissue. “The... the LITEs to control gene expression in mouse neurons and in the brains of living mice both quickly and...clear application to anyone with an interest in neuroscience, their use is by no means limited to particular... want to apply LITEs for the transformation of neurons, she offers a tip: save yourself some time by making...

  8. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...for neuronal systems. To develop a new single-component tool for magnetogenetic control of neurons, the...pTALYM4 Boris Greber pTAL7a, pTAL7b Michal Zurovec pBlue-TAL Nathan Lawson pJDS Series   Superglue...McMahon et al., report using as few as 150 fly neurons as their starting material! Thus, TRIBE can be ...non-invasive and can rapidly and reversibly stimulate neurons. Recent studies have designed multi-component actuators...finding that it could increase the activity of neurons from brain slice preparations. Magneto2.0 was also...Magneto 2.0 allows for magnetic remote control of neuronal activation. Find Magneto 2.0 plasmids by following...following the links below. Wheeler M, et al. Nat. Neurosci 2016. PubMed PMID: 26950006 Plasmid ID Plasmid...

  9. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    ...Detection of Norepinephrine. Neuron, 102(4), 745-761.e8. https://doi.org/10.1016/j.neuron.2019.02.037 Kagiampaki...Neuromodulators like dopamine and norepinephrine have important functions in the brain but have been ... B, Representative images of HEK293T cells and neurons expressing nLightG or nLightR before/after application...dashed lines. Scale bars, 10 μm (HEK293T), 20 μm (neurons). Figure and caption adapted from Kagiampaki et...

  10. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    ...detect the neurotransmitters and other bioactive molecules is integral to the study of neuroscience. To this...improved detection for tracking large populations of neurons in wide-field imaging (Dana et al., 2018). See ...chemogenetic AAV for activating and inhibiting neurons. Minimally-tagged DREADD AAV from the Richie lab...with Cre for intersectional control of specific neuronal populations. We now offer a Flp-expressing AAV...

  11. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type
    Blog Post
    ...29] Photoreceptors [30] Otic hair cells [31] Neurons [32, 33, 34] You can find a variety of plasmids...have been successfully transdifferentiated into neurons and β-cells [40-42]. Reprogramming and transdifferentiation...S.C. Zhang, Directed differentiation of dopamine neurons from human pluripotent stem cells. Methods Mol ...human pluripotent stem cells to cerebral cortex neurons and neural networks. Nat Protoc, 2012. 7(10): p...pluripotent stem cells to mature functional Purkinje neurons. Sci Rep, 2015. 5: p. 9232. PubMed PMID: 25782665...Direct conversion of fibroblasts to functional neurons by defined factors. Nature, 2010. 463(7284): p....

  12. Hot Plasmids - May 2022

    Type
    Blog Post
    ... Nature Neuroscience. 2022. https://doi.org/10.1038/s41593-021-00969-4.   Increased neuronal expression...flexibility for in vivo proximity labeling Increased neuronal expression using Cre-mediated AAV Targeted Evolution...found at least two variants that display increased neuronal expression in the brain while having decreased...M-CREATE plasmids here!  Goertsen D, et al. Nature Neuroscience. 2022. https://doi.org/10.1038/s41593-021-00969...

  13. Hot Plasmids: Summer 2025

    Type
    Blog Post
    ...via airway administration. AAV11 can transduce neurons for retrograde labeling, making it a powerful tool... tool for circuit tracing and other neuroscience applications (Figure 4). Check out our blog post on AAV...enables potent retrograde labeling of projection neurons. Created with BioRender.com. Addgene has tested... Bentley While several viral tools can perform neuronal tracing, anterograde tracing poses challenges,...labeling across single synapses, a key attribute for neuronal tracing. Labeling was nontoxic and activity-dependent...

  14. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    ...the data is heavily dominated by the U.S. (42%), Europe (23%), and China (14%). Spikes in plasmid requests...majority of our plasmid orders come from the U.S. and Europe, we see a corresponding dip in plasmid request ...following hierarchical model: Because the U.S., Europe, and China account for over 75% of all of our plasmid...(the last date in our dataset) for the U.S. and Europe. To improve the stability of our model, we considered...scientists placing orders per day across regions, with Europe most impacted and China least impacted. The inferred...

  15. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...far-red genetically encoded indicators of neuronal activity. J Neurosci Methods 362, 109314 (2021). Yu, D. et... Two-photon image of miRFP670nano3-expressing neurons in the neocortex of a living mouse. Image courtesy...(2015). Shcherbakova, D.M., Stepanenko, O.V., Turoverov, K.K. & Verkhusha, V.V. Near-Infrared Fluorescent...improved monomeric infrared fluorescent protein for neuronal and tumour brain imaging. Nat Commun 5, 3626 (...

  16. What's New in CRISPR - December 2019

    Type
    Blog Post
    ...fluorescent tags in neurons. Their toolkit named ORANGE (Open Resource for the Application of Neuronal Genome Editing...CRISPR/Cas9-based toolkit for epitope tagging in neurons Harold MacGillavry’s lab developed a CRISPR-based...targeted genomic integration of fluorescent tags in neurons.  Read the paper in bioRxiv Find the ORANGE toolkit...

  17. Celebrate Open Data Day with Addgene’s AAV Data Hub!

    Type
    Blog Post
    ... in Frontiers in Neuroanatomy and summarized some considerations for precise neuronal targeting using ...Considerations For Precise Neuronal Targeting Using AAV Technologies Using AAV for neuronal tracing Resources...

  18. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    ...ion-based communication is not just for neurons. Non-neuronal cells do this too. It's just that for non-neural...you hear the phrase “action potential?” Firing neurons are likely the first thing that comes to mind. ...exhibits an inactivation maximum of 590 nm light. Aurora and Phobos optogenetic tools - A collection of ...

  19. Hot Plasmids - October 2020

    Type
    Blog Post
    ...Since the pioneering days of Golgi and Cajal, neuroscientists have developed and refined many tools to tease...understand the complex interworking of the 86 billion neurons in the human brain. But studying synaptic connections...generally very small, and can contain different neurotransmitters. Using genetically encoded fluorescent proteins...inhibitory synapses, respectively. Once expressed in the neuron, the FingRs bind to the endogenous synaptic proteins...

  20. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    ...Brain Research Through Advancing Innovative Neurotechnologies (BRAIN) Initiative Cell Census Network, the... Fig. 1: Antibody production methods    NeuroMab and others have an extensive library of monoclonal...mouse tissue and compare the IHC results to both NeuroMab and the Allen Brain Atlas. The comparison with...intuitive guide for checking their staining and neuroanatomy without the fear of referencing a wrong section...
Showing: 81 - 100 of 333 results