We narrowed to 1,149 results for: Gene
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TypeBlog Post... care about these details, but does follow the general structure and flow of the entire session. I myself... then see how many of them have requested from Addgene. It is always gratifying to see all the hands go...Herminia Ibarra and Kent Lineback Resources on the Addgene Blog Check Out Our Networking for Scientists Blog...
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CRISPR Plasmids and Resources
TypeCollection...transcription. Epigenetics dCas9 fused to an epigenetic modifier alters chromatin structure/gene expression...Activate dCas9 fused to an activator peptide activates gene expression. Interfere dCas9 alone, or fused to a...factors. gRNAs gRNA plasmids to target specific genes. Browse Plasmids by Model Organism Bacteria CRISPR...CRISPR? Experts cover CRISPR topics on Addgene's blog. Addgene's Viral Service : Request ready-to-use viral...Cas enzymes generate double strand breaks (DSBs). Nick Mutated Cas nickase enzymes generate single-strand...CRISPR plasmids available at Addgene. Search by species and CRISPR function. CRISPR...technologies are continously developing and expanding. Addgene works with leading scientists in the field to ensure... -
Is this the right place for me? 8 tactics for choosing a lab
TypeBlog Post... scientists Resources on Addgene.org Learn about open positions at Addgene Check out our career videos...that loves you back. Additional resources on the Addgene blog Check out our transferable skills guide Learn... -
Allen Institute for Cell Science Plasmid Collection
TypeCollection... Endogenous Gene Tagging in Human Cells on the Addgene Blog. Plasmids for Endogenous Gene Tagging in Human...Link opens in a new window) RNP transfection for gene editing human iPS cells (Link opens in a new window..., S. M., & Gunawardane, R. N. (2017). Systematic gene tagging using CRISPR/Cas9 in human stem cells to... The plasmids used to generate these lines have been deposited here with Addgene for distribution to the...Institute for Cell Science has deposited plasmids to generate fluorescently tagged proteins of key cellular ...With these plasmids researchers will be able to generate fluorescently tagged proteins of key cellular ... with endogenously-tagged cellular structures. Addgene ID Plasmid Allen Institute ID Tag Protein Structure... -
Botman-Teusink Yeast FP Collection
TypeCollection...marker. ID Plasmid Gene/Insert Selectable Marker Publication Additional Resources Addgene Kits MoClo-YTK ...constitutive overexpression of FPs and tagging of genes of interest with FPs in yeast. Characterization ...Bydder, S. F., Zhou, Y., Nielsen, L. K. (2013). Dual gene expression cassette vectors with antibiotic selection...Overexpression (MyLO) CRISPR-Cas9 Toolkit Other Addgene Plasmids Bas Teusink Lab Materials Kurt Thorn Lab... -
Deep Dive: Statistical Tests (Comparisons)
TypeBlog Post...Parametric tests Statistical tests fall into two general categories: parametric and non-parametric. In this...returning false positives/negatives, but they all generally yield a similar result and are appropriate to ...common parametric comparison tests along with some general statistics knowledge to have in your tool belt.... -
Which Fluorescence Microscopy Technique is Best for Me?
TypeBlog Post...PubMed PMID: 26186186. Additional Resouces on the Addgene Blog Lightsheet Microscopy Choosing Your Fluorescent...Fluorescent Protein for Your Experiments Resources on Addgene.org Browse Our Fluorescent Protein Resources Find...Fluorescent Protein Backbones Find Plasmids for Optogenetics ... -
Malate Dehydrogenase CUREs Community Collection
TypeCollection...a diverse set of wild-type malate dehydrogenase genes and watermelon mutant clones for your course-based...interactions. Plasmids A diverse set of wild-type MDH genes from several species and mutant clones from watermelon..., or "subunit interface") ID Plasmid Description Gene/Insert Mutations Additional Resources MDH CUREs ... -
Synthetic Biology - Overview
TypeCollection...contains pre-assembled genetic circuits such as logic gates and higher level gene networks. Sensing and... pathways and auxiliary components. Networks and Gene Regulation Browse plasmids containing both naturally...Lab RNA Feedback Lu Lab Multiplexed/Programmable Gene Networks Mascher Lab Bacillus BioBrick Box Mukhopadhyay...about synthetic biology resources available at Addgene. Browse featured collections or depositing labs...engineering of biological systems for novel functions. Addgene is working with leading synthetic biologists around...facilitate the sharing of parts and information. Addgene can help you to share your synthetic biology plasmids...signaling and environmental sensing. Strains Browse Addgene's collection of engineered bacterial strains, including... -
Synthetic Biology - Sensing and Signaling
TypeCollection...Metabolism Networks & Gene Regulation Sensing & Signaling Strains Browse Addgene's collection of synthetic...plasmids available from this depositor's lab. Plasmid Gene/Insert Vector Type Promoter Tags PI Publication ...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!... -
Synthetic Biology - Strains
TypeCollection...Metabolism Networks & Gene Regulation Sensing & Signaling Strains Browse Addgene's collection of synthetic...available from this depositor's lab. Plasmid Description Gene/Insert Antibiotic Resistance PI Publication Back...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!... -
Synthetic Biology - Metabolism
TypeCollection...Metabolism Networks & Gene Regulation Sensing & Signaling Strains Browse Addgene's collection of synthetic...plasmids available from this depositor's lab. Plasmid Gene/Insert Vector Type Promoter PI Publication Back ...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!... -
TREAT-AD Plasmid Collection
TypeCollection...this table based on plasmid name, gene/insert, and more. ID Plasmid Gene/Insert Vector Type Backbone Mutations...of drug targets in Alzheimer’s disease (AD) by generating validated resources for emerging targets in AD...IUSM-Purdue TREAT-AD centers will be deposited at Addgene and available to request. Plasmids Browse the table... -
Zinc Finger Consortium Reagents
TypeCollection...Arrays for Zebrafish Genes - Browse pre-constructed zinc fingers targeting specific genes Oligomerized Pool...Keith Joung and Daniel Voytas have deposited at Addgene various reagents for engineering and expressing... -
CRISPR Guide Update
TypeBlog Post...Addgene is proud to present our updated CRISPR Guide! The CRISPR Guide helps scientists understand how...offer. Happy CRISPR-ing! More resources on the Addgene blog PRIME Editing CRISPR 101: Cas9 vs the Other... -
Retrovirus Plasmids
TypeCollection...your gene fused to eGFP expression Clevers 18760 MSCV IRES Luciferase MSCV Plasmid for transgene expression...transfer plasmids. Want a retroviral plasmid with your gene of interest? Search our database for retroviral ...Puro IRES GFP empty plasmid) MSCV For cloning and gene expression; select with puromycin or screen for ...'s genome, which can lead to oncogenesis (termed insertional mutagenesis). Read our γ-Retrovirus Guide...Learn about retroviral plasmids at Addgene, including popular packaging, envelope, and transfer plasmids...virus uses the reverse transcriptase in tow to generate a DNA provirus. This provirus then gets integrated...to ∼8 kb), and infection results in long-term transgene expression. Some disadvantages of γ-retroviruses... -
Synthetic Biology - Cloning and Genomic Tools
TypeCollection...Metabolism Networks & Gene Regulation Sensing & Signaling Strains Browse Addgene's collection of synthetic...available from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication Back to Top Shuttle...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!... -
#Deckthelab Contest Winners 2017
TypeBlog Post...this year! All winners receive an Addgene care package with Addgene mugs and some delicious hot chocolate... -
Congrats to the 2019 Deck The Lab Winners
TypeBlog Post...EikeMahlandt @AnoeskaMoosdijk @Saskia_deMan @SILS_UvA @addgene #SciArt #fluorescence #LCAM #DeckTheLab pic.twitter.com...holiday season. Santa frog is coming to town. @Addgene @UMich_MCDB #christmastaco #frogsnowflake #xenopus... -
Antibody Data Repositories and Search Engines
TypeBlog Post...Human Cell Differentiation Molecules group Addgene Data Hub Any (see notes) Any Data repository...repository Only accepts data for antibodies in Addgene’s repository IBEX Knowledge-Base Any IBEX...