We narrowed to 989 results for: IGH@;

...Incubate at room temperature for 2hr, or at 16°C overnight (following the manufacturer’s instructions). Note...For many ligation reactions, especially if using "high concentration" ligase, 5min at room temperature ...

...protocol may need to be adjusted to accommodate slight differences between products. Addgene does not ... 0.2 µm PES syringe filter. Aliquot and freeze upright at -20 °C. Procedure Section 1: Seeding cells The...

...viral particles. Users may need to run lower or higher dilutions depending on their particular sample....appropriate PCR tubes. Pipette back and forth 5 times. Lightly cap the PCR tubes. Generate the Droplets Bring ...

...C for 1 hour, you can try incubating at -80°C overnight. Centrifuge for 20 minutes at 10,000 x g at 4°...

...well enough to be snug to your hand, but not so tight that you cannot perform your duties. Be sure to ...

...#3. Incubate plate with newly plated bacteria overnight (12-18 hours) at 37 °C. Pro-Tip Some plasmids ...

... 6 cm diameter LB/agar plates without antibiotic High concentration (100 mg/mL, 1000x) carbenicillin stock...

...Addgene Depositing Page Career Videos Get career insight and advice from real Addgenies! (Link opens in ...

...also be used for PCR-mediated DNA mutagenesis. A higher cation concentration increases the error rate of...