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Showing: 501 - 510 of 960 results
  1. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    ...interview or whether your receive the next grant you apply for. After all, the only value your work will have... slide, think about whether the same principle applies to other slides in your deck.  If someone asks ...stay tuned to our website and social channels to apply for our future events. In addition to this annual...
  2. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

    Type
    Blog Post
    ... exon skipping. Previous CRISPR exon-skipping approaches used two guide RNAs to remove a given exon from...proof-of-concept for pre-clinical and clinical applications of dCasRx. The identification of Type VI Cas13d...sharing will continue to grow, and we at Addgene appreciate our depositors for making their tools available...
  3. A Deep Dive into BioBuilder

    Type
    Blog Post
    ...labs, BioBuilder inspires students and teachers to apply engineering principles to biological systems. Today...teams around the world who combine engineering approaches and scientific know-how to design/build/test ...students include The BioBuilder High School Apprenticeship, preparing local (Massachusetts), economically...
  4. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

    Type
    Blog Post
    ... protein purification with OptoNBs The first application for OptoNBs takes us in vitro: light-controlled...require a protein to be tagged, which limits these approaches to use with recombinant proteins. Purifying an...OptoNBs to control cell signaling The second application for OptoNBs takes us into cells: light-based ...
  5. From Lab Bench to Leadership: Transitioning into Management as a PhD

    Type
    Blog Post
    ...guidance and structure. I quickly learned that my approach needed to adapt based on individual needs. For... and disengagement. By tailoring my leadership approach to each person, I was able to create a more productive...tension between two team members with differing approaches—one detail-focused and the other big-picture ...
  6. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

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    Blog Post
    ...usable product with two 5′ overhangs, so these approaches require higher concentrations of insert to succeed...intermediate won’t just have nicks: it may have gaps or overlapping regions of single-stranded DNA. But as long as...of multicomponent assembly, as fragments with overlapping sequence homology can be assembled in a unique...
  7. Viral Vectors 101: Viral Vector Elements

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    Blog Post
    ...infectivity. Next, the virus must assemble and have appropriate factors displayed on the outside of the particle... production, they can be helpful for certain applications and aid in ease of use. Our lentiviral guide...elements discussed are compiled for reference below. Happy virus making!  References and Resources Kulanayake...
  8. Viral Vectors 101: Biosensors

    Type
    Blog Post
    ...ready-to-use. While biosensors span the gamut of applications within chemistry, biology, and physics, in this... this blog we will focus on those which can be applied in cell culture and animal models primarily. Fun...Neurotransmission and Neuromodulation: Engineering and Applications. Frontiers in Cellular Neuroscience. 2019 Oct...
  9. Antibodies 101: Isotypes

    Type
    Blog Post
    ... and remove a pathogen. Different isotypes also appear at different stages of an immune response. There... isotype of an antibody so you can select an appropriate isotype control. An isotype control has the same... Isotype controls are used in antibody-based applications including flow cytometry, immunohistochemistry...
  10. Developing a cold tolerant E. coli using specialized chaperones

    Type
    Blog Post
    ...can firmly gain a handle on understanding and appreciating their biology. The importance of psychrophilic... the empty vector. And thus was born ELSA - our happy lil’ cold-tolerant E. coli! Additionally, we’re ...re also working towards widening the applicability of our strategy using the pMBP-LbCas12a and pC013-Twinstrep-SUMO-huLwCas13a...
Showing: 501 - 510 of 960 results