...not just impact. Cause #3: Poor experimental design It’s easy to get excited about positive results. Not...easier to find information about research tools. BenchSci collates information about antibodies while FPbase...variety of additional resources and information about organizations promoting reproducibility in this ...that make it easier to share in-depth information about reagents, protocols, analysis tools, and more. Being...from a freezer. As you’ll see in Steve Almo’s talk about work at the Institute for Protein Innovation (IPI... talked to many researchers who have complaints about the unreliability of antibodies. IPI is working ...FPbase curates information about fluorescent proteins so you can best apply these technologies to your own...

... or isopropanol 90% ethanol 70% ethanol TE buffer 3 M Na-acetate (pH 4.8) Protocol: Generalized DNA Purification....5 volumes 95% or 100% ethanol and 1/10 volume of 3 M Na-acetate (pH 4.8). Invert the microfuge tube to... supernatant out of the tube if you are worried about losing the pellet. Dry with vacuum or by inverting...

...separated by size, (2) transferred to a membrane, and  (3) detected using antibodies.   Figure 1: Overview... the thickness of the band provides information about the relative abundance of the protein in the sample...history, principles, and types of ELISA, and our laboratory experience with peptide/protein analyses using...

...Originally published May 3, 2017 and last updated Sep 24, 2020 This post was contributed by guest blogger...target should be near the TSS but you can worry less about sequence optimality because you generally have fewer...

...figures for scientific publications. PLOS Biology, 19(3), e3001161. https://doi.org/10.1371/journal.pbio.3001161...to label the rat brain sample shown in Figure 2A above, Andrews et al. used the following combination of...

... the last section of the plate, to create streak #3. Incubate plate with newly plated bacteria overnight...resistance and your initials. Labeling within a laboratory setting is important for organization, and it...a section of the plate, as shown in the diagram above, to create streak #1. Pro-Tips Hold your tooth pick...

...Addgene's plasmid information pages recommend 5’ and 3’ sequencing primers. These primers typically anneal...Addgene lists the primers used to obtain each result above the posted sequence in the "View Sequence" link....sequence than expected and wish to contact Addgene about the accuracy of your plasmid, please email help@...

...this field, most jobs needed at least 3 months experience working in a developing tropical country. This...really passionate about during my degree. I loved parasitology. However, when I enquired about roles in this...with an overview of my experience and how I felt about the different positions to enable them to decide...

...and can exhibit recombination levels as high as 1-3%. In light of this finding, we now include guidance...the most common concerns we get here at Addgene about this system is off-target or spurious expression...cells that don’t express Cre or Flp, what can we do about it?  Test the serotype and promoter The first step...

...if you are using disinfectants as described above in step 3. You will also need to maintain the appropriate... lab! Introduction A water bath is a piece of laboratory equipment that helps bring your materials to ...