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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 1,082 results for: TOR;

Showing: 1001 - 1020 of 1082 results

  1. Synthetic Biology - Networks and Gene Regulation

    Type
    Collection
    ...Examples Include: promoters and terminators repressors and activators logic gates Networks and Gene Regulation...plasmids available from this depositor's lab. Plasmid Gene/Insert Vector Type Promoter PI Publication ...includes both naturally ocurring and synthetic regulatory elements, pre-assembled genetic circuits, and...

  2. TALENs for Endogenous Zebrafish Genes

    Type
    Collection
    ...TGGATGGCGAGAACAGGGcggtggagtataacgcTGTGGGCCTGACGAAGGA Complement Factor H TAL3230 & TAL3231 TGATTACATGTGAACCTCcatcacgtgaccagcatgTGTCCACCTCCTTTTGGA...TAAACGCCCTGATCTTCAgcgccgacggcctgcagcAGAGAGACAAGATGCGCA inhibitor of DNA binding 3 TAL3466 & TAL3467 TCAACATTGGATATCTATagagctttacagctagTTTATCCCAGTGTCCAGA...TCATGGTGCAGCCGTTACtggacagccgcgttccCTACGGACGCCTCCAGCA Purinergic receptor Y2 TAL3348 & TAL3349 TTCAACGCATCAGTGAATgttcaaaaccacagttCTGCACACAGCTGCAGCA...TCTTTTTGTGCTGCGCCAcccagcccataatcgaGTCTTCTACAGGCATGAA retinitis pigmentosa GTPase regulator b TAL3528 & TAL3529 TGGAGAAACAGAAGATGAaatccccgagtccggaGCTGTCTTCACTTTTGGA...TGGAGAAACAGAAGATGAaatccccgagtccggaGCTGTCTTCACTTTTGGA retinitis pigmentosa GTPase regulator b ORF15 TAL3530 & TAL3531 TAAGAGGGCGGAGCTTTTatctcgcaaagctctgCCCACTGAGTTACTTAGA...TGACTGGGAGGAAGATCAgagtcccgttgtgtctTGCAGCTCAGGATTTGGA Vascular-endothelial growth-factor-aa (vegfaa) TAL3200 & TAL3201 TCTCGCTTTGCTTCCTCCtcagtgattcccttcaTGGATGTGTATAAAAAGA...

  3. CRISPR Plasmids - Activate Gene Expression

    Type
    Collection
    ...gRNA sequence to direct the dCas9-activator to promoter or regulatory regions of your gene of interest....-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...Catalytically dead dCas9 fused to a transcriptional activator peptide can increase transcription of a specific...separate gRNA expression plasmid to target the dCas9-activator to your specific locus. Want more information ...

  4. CRISPR Plasmids - Zebrafish

    Type
    Collection
    ...Plasmid Gene/Insert Vector Type Promoter PI Publication Empty gRNA Expression Vectors Select a gRNA expression...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...expression plasmid based on factors such as selectable marker or cloning method. When using CRISPR, you...both the gRNA and Cas protein act as all-in-one vectors, but their function is often limited to a single...

  5. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...plasmids to study the regulatory networks involved in retinal development.... Depositor Collections Brzezinski...mouse retina. To aide in their studies of the regulatory networks involved in retinal development the ...Prdm1 and Vsx2 enhancers in the retina alters photoreceptor and bipolar cell fate specification, yet differs...

  6. Resolute Plasmid Collection

    Type
    Collection
    ... SLCs in Gateway-compatible pENTR vectors (pDONR221). These vectors allow a fast and efficient sub-cloning...framework that provides biological context. Assays monitoring impedance, fluorescence, mass/charge properties... into a wide range of Gateway-compatible pDEST vectors. ID Plasmid Gene/Insert Industry PI Pooled Libraries...the Resolute Partnership using their RESOLUTE repository feedback form (Link opens in a new window) . ...

  7. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ...Blog: Plasmids 101-Viral Vector Elements Blog: AAV use in CRISPR Viral Vectors 101 eBook Packaging Plasmids...Pseudotyping Want to save time? Check out our Viral Vector Packaging Service and learn about our options for...RepCap Plasmids for Serotypes Available as a Viral Vector Packaging Service at Addgene ID Plasmid Capsid ...species, gene name, and more. ID Plasmid Gene/Insert Vector Type Backbone Mutations Tags PI Do you have suggestions...

  8. Open Enzyme Collection

    Type
    Collection
    ... Depositor Collections FreeGenes Project Open Enzyme Collection...Enzymes You may also like… Bacterial Expression Vectors Synthetic Biology Plasmids BIOFAB Plasmid Set Drew... own enzymes by cloning them into an expression vector of their choice and transforming into E. coli bacteria...Researchers should clone these enzymes into an expression vector of their choice and then transform into E. coli...expression and purification ( pET28a-LIC or other pET vectors are recommended). The selection marker for all ...

  9. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...Protein Collection . Return to top Secretory Pathway The secretory pathway is named for its role in secreting...Posts Background General Cytoskeletal Junctions Secretory Pathway Background The subcellular localization... shipped. Many of the proteins that enter the secretory pathway require chaperones to help with folding... or search the table below to find markers of secretory pathway components. ID Plasmid Structure Targeting... 24920 pEGFP-LC3 (human) Autophagosome LC3 EGFP Toren Finkel 14435 mRFP-Clc Clathrin-coated vesicles Clathrin...

  10. Biosensor AAV Preps

    Type
    Collection
    ... Viral Vector Packaging Service AAV Biosensors Viral Vector Packaging Service: Biosensor...CAG CaMKIIa Dlx EF1a GFAP/GfaABC1D Synapsin E2 regulatory element Activity Cre-dependent Flp-dependent ...Karpova and Schaffer labs. See the Retrograde AAV Inventory Page . Don’t See What You’re Looking For? Our ... eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know...format. Please note this does not guarantee viral vector packaging service, but lets us know what viruses...

  11. Brain Armamentarium

    Type
    Collection
    ...will aid neuroscientists in studying circuits in laboratory animals and human tissue specimens. These technologies...rAAV-hI56i-minBglobin-iCre-4X2C-WPRE3-BGHpA AAV vector for Cre expression in forebrain GABAergic interneurons...lines or co-infection with Cre dependent viral vectors. Jonathan Ting Viviana Gradinaru 163509-PHPeB CN1839...CN1839-rAAV-hSyn1-SYFP2-10aa-H2B-WPRE3-BGHpA AAV vector for nuclear SYFP2 labeling in neurons. Useful for...BGHpA DLX2.0 (3xCore version of DLX enhancer). AAV vector for strong & rapid SYFP2 expression in forebrain...

  12. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ... Depositor Collections Rinehart Lab Phosphoprotein Reagents...Library #111704) is anticipated to be useful for laboratories performing phosphoproteomic experiments in need...can also be introduced into a second expression vector encoding a split mCherry fluorescent reporter, ...domain and the phosphosite, mCherry fluorescence is restored, so interactions can be discovered by fluorescence-activated... via partial UAG codon reassignment and release factor 1 deletion . FEBS Letters , 586 (20), 3716–3722...

  13. Cell Migration Consortium Plasmids

    Type
    Collection
    ... Depositor Collections Cell Migration Consortium (CMC) Plasmids...Cell Migration Consortium and Addgene. Member laboratories of the Cell Migration Consortium have deposited...for cell migration research at Addgene. CMC investigators who have deposited plasmids include: Joan Brugge...Francisco Browse Plasmids ID Plasmid Gene/Insert Vector Type Backbone Mutations Tags PI...

  14. Impact of Genomic Variation on Function (IGVF) Consortium Collection

    Type
    Collection
    ... plasmid tools generated by IGVF Consortium investigators to examine how genomic variation affects genome... Depositor Collections IGVF Consortium Impact of Genomic Variation...collaborative program that brings together teams of investigators who will use state-of-the-art experimental and...plasmid tools through Addgene. ID Plasmid Description Vector Type PI Publication Additional Resources The IGVF...

  15. CRISPR Plasmids - Xenopus

    Type
    Collection
    ...Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors such as selectable marker...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...both the gRNA and Cas protein act as all-in-one vectors, but their function is often limited to a single...

  16. Fluorescent Protein Guide

    Type
    Collection
    ...is a Monomer not a Monomer? Addgene's plasmid repository contains a variety of fluorescent protein plasmids...conformational changes within a protein. Biosensors Monitor small biomolecules or other physiological intracellular...signals, cells, or groups of cells with either actuators or sensors. Chemogenetics Plasmids | Guide Use...molecules to activate genetically engineered cellular receptors that affect signalling pathways within cells. ...

  17. CRISPR Plasmids - CRISPR Transposases (CAST)

    Type
    Collection
    ...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...fragments of DNA at specific locations. The Cas effector lacks nuclease activity, instead recruiting the... and type V-K. Type I systems use a type I Cas effector, composed of several separate Cas enzymes that...target site. Type V-K systems use a type V-K Cas effector, Cas12k, and three transposase components (TnsB...

  18. Addgene Packaged on Request: Scope of Service

    Type
    Collection
    ...any viral vector. Addgene performs QC measures which verify the identity of the viral vector, but not ... Viral Vector Packaging Service Packaged on Request Scope of Service AAV Packaged on Request: Scope of...guarantees above. We will have our expert viral vector production team carefully review your cargo/serotype...

  19. Antibody Production

    Type
    Collection
    ...antibodies (rAb) encoded by mammalian expression vectors, human embryonic kidney cells are transfected with...Neuromab Protocols (Link opens in a new window) page. Storage and Shipping Stability Addgene does not recommend... suggests preparing single-use aliquots before storing at -20 °C. Each antibody catalog item is tested...some of our other resources to help you choose, store, or use your antibody. Antibody Blog Posts Recipient...

  20. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...s reverse complement for cloning into the pX330 vector using BbsI restriction enzyme ( Table 3 ). Add ...sgRNA expression from the U6 promoter of the pX330 vector is enhanced by the inclusion of a G nucleotide ...Prepare a 50 μl reaction mix: 100 ng circular pX330 vector, 1.0 μl annealed oligos (1 μM; see step 3.1.4 )... ( e.g., with a PCR cloning kit) into a plasmid vector. Transform the plasmid into DH5α E. coli cells ...
Showing: 1001 - 1020 of 1082 results