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We narrowed to 960 results for: App;

Showing: 511 - 520 of 960 results

  1. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ...) How many loci can Cas9 modify? To test the application of this system to gRNA multiplexing, Xie et al...multiplexing further illustrates the potential of PTGs. Applications of PTGs The major advantage of the PTG system...multiple gRNAs may be used to target genes with overlapping activity. Multiple gRNAs can also facilitate ...

  2. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    ...associated with molecular plant parts, particularly for applied projects intended for commercialization. Many of...patented, and building our own novel pieces where applicable. We will also be working with open source, transformation...but there are several hazards to navigate when mapping out a new project. We’ve shared some of the concerns...

  3. Will You Be My Mentor? Finding and Asking for Mentoring Support

    Type
    Blog Post
    ... I will describe some of the many ways you can approach finding someone to give you advice and guidance...see someone with a great professional presence, approach them for advice on what makes them that way. Is...organization has a mentoring program, consider tapping into it and helping make it work by being an educated...

  4. Antibodies 101: Conjugation

    Type
    Blog Post
    ...especially if you start to consider clinical applications). But there are a few other classes that we ...proximity ligation, immuno-PCR, and single-cell applications (Hegazy, et al., 2020; Niemeyer, et al., 2007...al., 2015). However, there are certainly other approaches that involve modifying the antibody sequence ...

  5. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ...experiments. Recombinant AAVs (rAAVs) The primary application of AAV is delivery of genetic cargo to a host...rates for AAVs are generally high enough for this approach to work, although it’s less effective in vivo ... S., et al. “Various AAV Serotypes and Their Applications in Gene Therapy: An Overview.” Cells, 12(5),...

  6. The time and cost required to make a plasmid

    Type
    Blog Post
    ...screens and the more traditional “one at a time” approach. According to his math, it takes five hours of...method is free for you, and many labs are quite happy to share, labs that get high volumes of plasmid ...or changes. Fun fact: Addgene’s high-throughput approach means we virtually always use every single well...

  7. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    ...permanent control mechanism? Where is the control happening? Is the regulation of an exogenously introduced...times) into the genome. Make sure to choose an appropriate promoter to express your cDNA, depending on the...flavors of activators which can be used with this approach - read more about them on our CRISPRa blog! ...

  9. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    ... changes in neural activity when paired with appropriate fluorescent optics. Existing red fluorescent ...organisms such as E. coli and yeast. Because this approach uses standardized parts, it allows for greater...increasingly been recognized for its biotechnological applications including conversion of CO2 into water and electrical...

  10. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    ... work on our external website appearance and functionality are happening and this strengthens our image...support more new projects. Diversification is happening. The Addgene team will have some fun challenges...
Showing: 511 - 520 of 960 results