We narrowed to 532 results for: abo.3

... the last section of the plate, to create streak #3. Incubate plate with newly plated bacteria overnight...resistance and your initials. Labeling within a laboratory setting is important for organization, and it...a section of the plate, as shown in the diagram above, to create streak #1. Pro-Tips Hold your tooth pick...

...Addgene's plasmid information pages recommend 5’ and 3’ sequencing primers. These primers typically anneal...Addgene lists the primers used to obtain each result above the posted sequence in the "View Sequence" link....sequence than expected and wish to contact Addgene about the accuracy of your plasmid, please email help@...

...spectra Example: Jaws Archaerhodopsins Archaerhodopsin-3 (Arch) from Halorubrum sodomense is also commonly ...with single-spike temporal resolution. PloS One, 2 (3), e299. https://doi.org/10.1371/journal.pone.0000299... of distinct neural populations. Nat Methods, 11 (3), 338–346. https://doi.org/10.1038/nmeth.2836 PMID...of neural activity. Cold Spring Harb Protoc, 2011 (3), top102. https://doi.org/10.1101/pdb.top102 PMID:... to permit CIB1 binding. As shown in the figure above, this light induced binding can be utilized to control...dimerization. In addition to the applications described above, both LOV and Dronpa can control activity of a fused...

...this field, most jobs needed at least 3 months experience working in a developing tropical country. This...really passionate about during my degree. I loved parasitology. However, when I enquired about roles in this...with an overview of my experience and how I felt about the different positions to enable them to decide...

...if you are using disinfectants as described above in step 3. You will also need to maintain the appropriate... lab! Introduction A water bath is a piece of laboratory equipment that helps bring your materials to ...

...and can exhibit recombination levels as high as 1-3%. In light of this finding, we now include guidance...the most common concerns we get here at Addgene about this system is off-target or spurious expression...cells that don’t express Cre or Flp, what can we do about it?  Test the serotype and promoter The first step...

...nanobodies. Nature Structural & Molecular Biology, 25(3), 289–296. https://doi.org/10.1038/s41594-018-0028...structure-guided directed evolution. The nice thing about these methods is that they all follow the same general...performance. If you are interested in learning more about antibody engineering technologies, the Institute...

...Timeline Day 1: Antigen Coating Day 2: Blocking Day 3: Primary antibody incubation Day 4: Secondary antibody...at room temperature or overnight at 4 °C . Section 3: Primary antibody incubation Carefully remove the ...point. If your unknown sample’s absorbance falls above the range of the standard curve you will need to...

...Originally published May 3, 2017 and last updated Sep 24, 2020 This post was contributed by guest blogger...target should be near the TSS but you can worry less about sequence optimality because you generally have fewer...

...Science 6.1 (2015): 50-69. Royal Society of Chemistry. 3. Ermolenko, Dmitri N., et al. "Observation of intersubunit...environment? If these are pressing issues in your laboratory, then FRET experiments are a viable biophysical...to consider these cases separately when talking about the tools that are available. Let’s consider the...that, if you make an FP fusion, you are inserting about 20-25 kDa of protein mass into your protein and ...together The FRET donors and acceptors described above can be paired together to measure a wide range of...of your protein that can lead to novel insights about its biology and its behavior. See the table below...