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1. Addgene's eBook Collection

   Type

   Guide

   ...Select Science Career Guide November 2018 | First Edition About Science Career Guide November 2018 |...your gRNA and how to use pooled libraries in genome-wide screens. Close About Overlay Select Fluorescent ...Vectors 101 July 2024 | Second Edition About This guide to viral vectors contains an introduction to each... | First Edition About The guide includes information on the many career options in science, advice ...advice on mentoring and management, resources for identifying your transferable skills, and tips on the job...

2. Handling Plasmids from Addgene - Purifying Plasmid DNA

   Type

   Protocol

   ...Pro-Tip DNA resuspension can take time, it is a good idea to let it sit for several hours to overnight at ...at 4°C. Tips and FAQ Plasmid purification kits provide the fastest way to obtain a high concentration ...

3. Promoters

   Type

   Guide

   ... are located within promoter regions, and they provide a stable binding site for RNA polymerase and transcription...Pribnow box which usually consists of the six nucleotides, TATAAT. Proximal Promoter Further upstream from...prokaryotic promoters. Eukaryotic promoters span a wide range of DNA sequences. It is not unusual to have...

4. Sequencing Primers

   Type

   Guide

   ...Primers? The primer sequences listed on the left are provided for your reference. Addgene does not distribute...primers based on the plasmid backbone and insert. To identify primers that may be useful in your sequencing ...

5. DNA Quantification

   Type

   Protocol

   ...affected the accuracy of the NanoDrop. It is a good idea to re-zero any spectrophotometer between samples...

6. Centrifugation

   Type

   Protocol

   ...Intro to the Lab Bench Check out more protocols and videos to help you get started in the lab! Introduction...