...Biering SB,  Sarnik SA, Wang E, Zengel JR, Sathyan V, Nguyenla X, Van Dis E, Catamura C, Yamashiro LH,  ...Puschnik AS, Bertozzi CR, Carette JE, Stanley SA, Harris E, Konermann S, Hsu PD (2021) Genome-wide, bidirectional...starting point for SARS-CoV-2 researchers to investigate how the virus interacts with human cells. Addgene...

...different strains of E. coli that can be used for transformation. The preferred E. coli strains for storage... DNA has been received and stored, it is put into E. coli strains that have been treated to easily take...kanamycin resistance gene. Every step along the way involves good aseptic technique and controls to ensure ...

...amino acids E. coli and Mammalian Cells pEVOL-pAzF synthetase for p-azido-l-phenylalanine E. coli pEVOL-pBpF...has proven most effective only in a few cell types (E. coli and Xenopus oocytes, mainly). Nevertheless, ...pEVOL-pBpF synthetase for p-benzoyl-l-phenylalanine E. coli pAcBac1.tR4-MbPyl pyrrolysyl-tRNA synthetase...commercially available as Lumio Green and Lumio Red from Invitrogen (now ThermoFisher Scientific). These reagents...structurally characterizing large ion channels involved in skeletal muscle contraction.   References 1...

...plasmid multimerization in the naturally occurring E. coli plasmid ColE1 (Goebel & Helinski, 1968; Bazaral... (Bedbrook & Ausubel, 1976; Fishel et al., 1981). E. coli strains such as DH5α, HB101, NEB Stable, Stbl3...multiple circular DNA forms of colicinogenic factor E-1 from Proteus mirabilis. Biochemistry. 1968 Oct;7...1566524020666200427110452. PMID: 32338220. Crozat E, Fournes F, Cornet F, Hallet B, Rousseau P. Resolution...during our quality control process. Since MiSeq involves sequence assembly from short reads (<300 bp) which...

...Lentiviral preps for expressing the E protein (pLVX-EF1alpha-SARS-CoV-2-E-2xStrep-IRES-Puro) and NSP2 (pLVX-EF1alpha-SARS-CoV...service in 2016, we’ve added many new tools to our inventory of ready-to-use viral vectors. Here are some of...interneurons (83895). See our Serotype Testing AAV inventory for the entire collection! Plasmid Serotype...Fishell   Chemogenetics Chemogenetics offers noninvasive methods for manipulating cell activity, particularly...

...Burke Lab (Roux et al., 2012). BioID utilizes the E. coli biotin ligase, BirA*, with a site mutation (...DNA-binding domain (233 amino acids) found in the E. coli version. It also contains a single mutation ...signal to noise ratio 30-fold compared to the standard E. coli BirA* mutant. RapID consists of this mutant ...Interactions through Spatial Proximity." Journal of Investigative Dermatology 137.12 (2017): e197-e203. PubMed...

...Ye C, Rogan SC, Adams AC, Cusher DS, Maratos-Flier E, Roth BL, Lowell BB. Rapid, reversible activation ...PubMed Central PMCID: PMC3069789. 3. Kügler S, Kilic E, Bähr M. Human synapsin 1 gene promoter confers highly...few inventory items offered domestically, but by the end of 2016, we expanded our viral inventory to 25... lentiCas9-Blast Most of Addgene’s lentiviral inventory is based on CRISPR tools, though we do provide...pAAV-hSyn-DIO-hM4D(Gi)-mCherry Our current AAV inventory consists almost entirely of viruses expressing... Carolina, Chapel Hill. These can be used to investigate the effects of activating particular signaling...neurons drives feeding behavior in mice. J Clin Invest. 2011 Apr;121(4):1424-8. PubMed PMID: 21364278....

... a supplemental add-on kit and is compatible with E. coli and/or cell-free systems. Protein expression... et al. “EcoFlex: A Multifunctional MoClo Kit for E. Coli Synthetic Biology.” ACS Synthetic Biology, vol...CIDAR MoClo: Improved MoClo Assembly Standard and New E. Coli Part Library Enable Rapid Combinatorial Design...//doi.org/10.1021/acssynbio.6b00337. Lee, Michael E., et al. “A Highly Characterized Yeast Toolkit for...model organisms and backgrounds, MoClo can be invaluable for more complex editing and screening experiments...

...polymerase I (PolI3M) is capable of mutagenesis in E. Coli, but this approach requires special bacterial...mutations per nucleotide per generation of wild-type E. coli, while only increasing the standard mutation...be used to map protein-protein interactions, investigate non-coding segments of the genome, or engineer...

...and strategies for their projects. References 1. L. E. Woodard and M. H. Wilson, “piggyBac-ing models and...Biol., Apr. 2016. PubMed PMID: 27075976. 4.  X. Li, E. R. Burnight, A. L. Cooney, N. Malani, T. Brady, J.... Menon, G. S. Parker, S. J. Qualls, B. M. Lewis, E. Ke, C. T. Dargitz, R. Wright, A. Khanna, F. H. Gage... likely due to the fact that PBx relies on the inverted terminal repeats (ITRs) found on the piggyBac™... Grégoire and M. Kmita, “Recombination between inverted loxP sites is cytotoxic for proliferating cells...