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  1. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

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    ...transposon-encoded CRISPR–Cas system. Nature 577:271–274 . https://doi.org/10.1038/s41586-019-1849-0 Klompe SE, Vo...RNA-guided DNA integration. Nature 571:219–225 . https://doi.org/10.1038/s41586-019-1323-z Peters JE, Makarova...transposons. Proc Natl Acad Sci USA 114:E7358–E7366 . https://doi.org/10.1073/pnas.1709035114 Rubin BE, Diamond... Bacteria Within Microbial Communities. bioRxiv https://www.biorxiv.org/content/10.1101/2020.07.17.209189v2...CRISPR-associated transposases. Science 365:48–53 . https://doi.org/10.1126/science.aax9181 Strecker J, Ladha...CRISPR-associated transposases.” Science 368:eabb2920 . https://doi.org/10.1126/science.abb2920 Vo PLH, Ronda ...multiplexed bacterial genome engineering. Nat Biotechnol. https://doi.org/10.1038/s41587-020-00745-y Zhang Y, Sun...

  2. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

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    Blog Post
    ... (e.g. http://openwetware.org/wiki/Protocols/Template). Nature Protocol Exchange Website: http://www.nature.com...share your lab protocols online Addgene Website: http://www.addgene.org/ If you’re working with plasmids...started on our deposit page. Protocols.io Website: http://protocols.io/ Protocols.io is the creation of ... to use the resource. OpenWetWare Wikis Website: http://www.openwetware.org/wiki/Protocols OpenWetWare.org... and biomedical topics. Protocol Online Website: http://www.protocol-online.org/ Protocol Online is one... find useful for your research. LabGuru Website: http://www.labguru.com/ Labguru is a good place to go...such as Nature Protcols and NEB. Quartzy Website: https://www.quartzy.com/ Quartzy is a free web-based lab...

  3. CRISPR 101: Cytosine Transversion Editors

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    ...outcomes and assisting sgRNA selection is available at http://www.sunlab.fun:3838/BE_SMART/. Even more new DNA...repair proteins. Nature Communications, 12(1), 1384. https://doi.org/10.1038/s41467-021-21559-9 Huang, M. E...translocation. Nature Cell Biology, 26(2), 294–304. https://doi.org/10.1038/s41556-023-01342-2 Koblan, L. ...learning. Nature Biotechnology, 39(11), 1414–1425. https://doi.org/10.1038/s41587-021-00938-z Koblan, L. ...reconstruction. Nature Biotechnology, 36(9), 843–846. https://doi.org/10.1038/nbt.4172 Kurt, I. C., Zhou, R....human cells. Nature Biotechnology, 39(1), 41–46. https://doi.org/10.1038/s41587-020-0609-x Yang, C., Dong..., Baden-Wurttemberg, Germany), 9(26), e2202957. https://doi.org/10.1002/advs.202202957 Yuan, T., Yan, ...

  4. A Needle in a Base-Stack: Cas9 Structural Biology

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    .... Nature Reviews Microbiology, 20(11), 641–656. https://doi.org/10.1038/s41579-022-00739-4 References ...surveillance by CRISPR–Cas9. Nature, 603(7900), 343–347. https://doi.org/10.1038/s41586-022-04470-1 Cofsky, J. ...Structural & Molecular Biology, 29(4), 395–402. https://doi.org/10.1038/s41594-022-00756-0 Jiang, F., ...Mechanisms. Annual Review of Biophysics, 46, 505–529. https://doi.org/10.1146/annurev-biophys-062215-010822 ...Guide RNA and Target DNA. Cell, 156(5), 935–949. https://doi.org/10.1016/j.cell.2014.02.001 Osuka, S., ... CRISPR–Cas9. The EMBO Journal, 37(10), e96941. https://doi.org/10.15252/embj.201796941 Pattanayak, V....specificity. Nature Biotechnology, 31(9), 839–843. https://doi.org/10.1038/nbt.2673 Sternberg, S. H., LaFrance...

  5. Plasmids 101: Plasmid Incompatibility

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    ...by low-copy number plasmids. Plasmid 80:54–62 . https://doi.org/10.1016/j.plasmid.2015.03.007 Pubmed PMID...clusters. Molecular Microbiology 56:1430–1440 . https://doi.org/10.1111/j.1365-2958.2005.04643.x Pubmed...resistance plasmids in vivo. PLOS ONE 12:e0172913 . https://doi.org/10.1371/journal.pone.0172913 Pubmed PMID...Using Plasmid Incompatibility. PLoS ONE 7:e29875 . https://doi.org/10.1371/journal.pone.0029875 Pubmed PMID...incompatibility. Letters in Applied Microbiology 47:235–240 . https://doi.org/10.1111/j.1472-765x.2008.02426.x Pubmed...Current Opinion in Structural Biology 22:72–79 . https://doi.org/10.1016/j.sbi.2011.11.001 Pubmed PMID:...and host taxonomy. Frontiers in Microbiology 6: . https://doi.org/10.3389/fmicb.2015.00242 Pubmed PMID: ...

  6. CRISPR/Cas9 FAQs Answered!

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    ...Nucleases. G3 Genes Genomes Genetics, 3(4), 657–664. https://doi.org/10.1534/g3.112.005439  Cong, L., Ran,...CRISPR/CAS systems. Science, 339(6121), 819–823. https://doi.org/10.1126/science.1231143  Elliott, B.,... Molecular and Cellular Biology, 18(1), 93–101. https://doi.org/10.1128/mcb.18.1.93  Ran, F. A., Hsu...genome editing specificity. Cell, 154(6), 1380–1389. https://doi.org/10.1016/j.cell.2013.08.021  Ran, F. ...CRISPR-Cas9 system. Nature Protocols, 8(11), 2281–2308. https://doi.org/10.1038/nprot.2013.143 Sander, J. D.... genomes. Nature Biotechnology, 32(4), 347–355. https://doi.org/10.1038/nbt.2842  Soldner, F., Laganière...Parkinson Point mutations. Cell, 146(2), 318–331. https://doi.org/10.1016/j.cell.2011.06.019  Wang, H.,...

  7. How to Design Your gRNA for CRISPR Genome Editing

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    ... and prime editors. Nat Biotechnol 38:824–844 . https://doi.org/10.1038/s41587-020-0561-9 Dempster JM,...CRISPR-Cas9 gene dependency data sets. Nat Commun 10: . https://doi.org/10.1038/s41467-019-13805-y Doench JG (...guide to genetic screens. Nat Rev Genet 19:67–80 . https://doi.org/10.1038/nrg.2017.97 Hanna RE, Doench JG...CRISPR–Cas experiments. Nat Biotechnol 38:813–823 . https://doi.org/10.1038/s41587-020-0490-7 Radzisheuskaya...transcriptional repression. Nucleic Acids Res 44:e141–e141 . https://doi.org/10.1093/nar/gkw583 Rees HA, Liu DR (2018...transcriptome of living cells. Nat Rev Genet 19:770–788 . https://doi.org/10.1038/s41576-018-0059-1 Veres A, Gosis...Whole-Genome Sequencing. Cell Stem Cell 15:27–30 . https://doi.org/10.1016/j.stem.2014.04.020 Yang L, Guell...

  8. Predicting Adverse Reactions to Monoclonal Antibody Drugs

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    ...Vulnerable Patients. Frontiers in Immunology, 13. https://www.frontiersin.org/articles/10.3389/fimmu.2022.885672Piché...Safety Assessment (pp. 519–542). Academic Press. https://doi.org/10.1016/B978-0-12-417144-2.00027-5van ... Challenges. The oncologist, 21(10), 1260–1268. https://doi.org/10.1634/theoncologist.2016-0061WHO. International...Enhancements. Frontiers in drug discovery, 2, 952326. https://doi.org/10.3389/fddsv.2022.952326Joubert, M. K...Antibody-Based Biotherapeutics. PloS one, 11(8), e0159328. https://doi.org/10.1371/journal.pone.0159328Groell, F....Pharmazeutische Verfahrenstechnik e.V, 130, 128–142. https://doi.org/10.1016/j.ejpb.2018.06.008Lu, RM., Hwang...treatment of diseases. J Biomed Sci 27, 1 (2020). https://doi.org/10.1186/s12929-019-0592-z  Resources Antibodies...

  9. RNA Interference in Plant Biology: New Tools for an Old Favorite

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    ... Function Phenotypes. Plant Biology 7:251–257 . https://doi.org/10.1055/s-2005-837597 Li J-F, Chung HS... Silencing in Plants. Plant Cell 25:1507–1522 . https://doi.org/10.1105/tpc.113.112235 Morgens DW, Deans...for essential genes. Nat Biotechnol 34:634–636 . https://doi.org/10.1038/nbt.3567 Nakaoka Y, Miki T, Fujioka...Microtubule Generation. Plant Cell 24:1478–1493 . https://doi.org/10.1105/tpc.112.098509 Smits AH, Ziebell...in CRISPR knock outs. Nat Methods 16:1087–1093 . https://doi.org/10.1038/s41592-019-0614-5 Sztal TE, Stainier...genetic robustness. Development 147:dev186452 . https://doi.org/10.1242/dev.186452 Wilson RC, Doudna JA...RNA Interference. Annu Rev Biophys 42:217–239 . https://doi.org/10.1146/annurev-biophys-083012-130404 ...

  10. Summer SciComm: Preprints

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    ...Resources References ASAPbio, Preprint Resource Center: https://asapbio.org/preprint-info ASAPbio, Preprints and... and Rapid Communication of COVID-19 research: https://asapbio.org/preprints-and-covid-19 Ettinger CL,...preprinting for early-career researchers. Biol Open. https://doi.org/10.1242/bio.059310 Fraser N, Mayr P, ...preprints: a survey of bioRxiv authors. bioRxiv. https://doi.org/10.1101/2021.09.07.459259 Sarabipour S...early career researcher perspective. PLoS Biol. https://doi.org/10.1371/journal.pbio.3000151 Sever R, ...bioRxiv: the preprint server for biology. bioRxiv. https://doi.org/10.1101/833400  Stern BM, O’Shea EK (2019...scientific publishing in the life sciences. PLoS Biol. https://doi.org/10.1371/journal.pbio.3000116 More resources...
Showing: 51 - 60 of 258 results