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We narrowed to 91 results for: lentiviral production

Showing: 51 - 60 of 91 results

  1. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

    Type
    Blog Post
    ...incapable of producing infectious virus (similar to lentiviral vectors) because they do not encode all of the...minigenome, thus modeling genome replication. These products can be detected by Northern blot analysis. In ...Addgene.org Browse Our Viral Vector Plasmids Read Our Lentiviral Vector Guide Read Our AAV Guide  ...

  3. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    ...transfection, and does not necessarily indicate the production of virus. Alternatively, if the virus encodes...packaging cells. Another method of ensuring viral production is to perform PCR against components of the viral...with, there’s a lot that goes into virus design, production, and transduction. Once you’ve harvested, it’...out Our Primer for Beginners! Learn All about Lentiviral Vectors Additional Resources on the Addgene....

  4. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

    Type
    Blog Post
    ...via a lentiviral vector to cell lines that either stably expressed Cas9 or that had been lentiviral infected...regulation, including almost all genes involved in the production of a cell surface glycan that’s required for ...cheat sheet Read our Quick Guide to All Things Lentivirus Resources on Addgene.org Check out this guide...

  5. Meet the New Addgene Board Members!

    Type
    Blog Post
    ...company.  Ultragenyx’s goal is to develop novel products for the treatment of rare and ultra-rare diseases...Harvard Medical School, he developed the first lentiviral microRNA-based system for polymerase II-regulated...

  6. Addgene: The First Twenty Years

    Type
    Blog Post
    ...again! Addgenie Marcy (now our Sr. Director of Production) pie-fully celebrating Addgene's 10 year anniversary...ready-to-use viral vectors from some of our most popular lentiviral and AAV vectors, as well as developing and sharing... recombinant antibodies, another plasmid-based product that, with Addgene’s support, could fill a community...

  8. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    ...genus capable of producing a variety of natural products. Recently, this group optimized their initial ... 10 guide RNAs per gene. The lab modified the lentiviral backbone to encode the CD90.1 selection marker...

  9. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    ...-studied lentivirus is HIV, which many lentiviral tools have been derived from. Lentiviruses have slightly...adenovirus has the highest maximum titer, a plus for production and infection. This virus can infect dividing... with immortalized cell line cultures. Lentivirus Lentivirus is a type of retrovirus and shares many ... commonly used lab viruses: gamma-retrovirus, lentivirus, adenovirus, and adeno-associated virus. Gamma-retrovirus...gamma-retrovirus, coming in around 9 kb. Unlike gamma, lentivirus can infect both dividing and non-dividing cells...nucleus as gamma-retroviruses do. Fun fact: Since lentivirus and gamma-retrovirus are so closely related, ...specific virus? We have you covered with guides on lentivirus, retrovirus, adenovirus, and AAV. If you’re wondering...

  10. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...precursors, biofuels, and natural products without the undesirable side products generated during chemical synthesis...these molecules. To make large amounts of pure product in an affordable way, researchers test many different... resistance through gene stacking, increasing production of a protein of interest, and improving the nutrient...Fragmid system was used to assess how different lentiviral plasmid backbone elements impact viral titer ...Modular Assembly Toolkit for the Rapid Design and Production of Diverse Expression Vectors.” ACS Synthetic...expand the Fragmid toolkit to facilitate other lentivirus- and AAV-based studies. Unified MoClo Systems...
Showing: 51 - 60 of 91 results