... mRFP Tsoulfas Lab Lentiviral Plasmids - Fluorescently tag your gene for lentiviral expression Verkhusha...Emission maximum are listed in nm. Brightness is the product of exctinction coefficient and quantum yield divided...Prone to dimerization pLV-Azurite - Mammalian Lentiviral expression pB18cmBFPAzurite - Bacterial Expression...pCAG-GFP - Mammalian Expression pLV-eGFP - Mammalian Lentiviral Expression Ac5-STABLE1-neo - Insect Expression... Mammalian Expression pLV-mCherry - Mammalian Lentiviral Expression pCS2+8CmCherry - Zebrafish/Xenopus...

...useful to the study of SARS-CoV-2 are the lentiviral production and packaging vectors VSV-G and psPAX2, ...to scientists around the world. This scale of production and distribution is possible due to the dedicated...

...transfection, and does not necessarily indicate the production of virus. Alternatively, if the virus encodes...packaging cells. Another method of ensuring viral production is to perform PCR against components of the viral...with, there’s a lot that goes into virus design, production, and transduction. Once you’ve harvested, it’...out Our Primer for Beginners! Learn All about Lentiviral Vectors Additional Resources on the Addgene....

...via a lentiviral vector to cell lines that either stably expressed Cas9 or that had been lentiviral infected...regulation, including almost all genes involved in the production of a cell surface glycan that’s required for ...cheat sheet Read our Quick Guide to All Things Lentivirus Resources on Addgene.org Check out this guide...

...company.  Ultragenyx’s goal is to develop novel products for the treatment of rare and ultra-rare diseases...Harvard Medical School, he developed the first lentiviral microRNA-based system for polymerase II-regulated...

...again! Addgenie Marcy (now our Sr. Director of Production) pie-fully celebrating Addgene's 10 year anniversary...ready-to-use viral vectors from some of our most popular lentiviral and AAV vectors, as well as developing and sharing... recombinant antibodies, another plasmid-based product that, with Addgene’s support, could fill a community...

...genus capable of producing a variety of natural products. Recently, this group optimized their initial ... 10 guide RNAs per gene. The lab modified the lentiviral backbone to encode the CD90.1 selection marker...

... The “Riboglow” platform for visualizing RNA production and localization Article contributed by Tyler...deaminases like APOBEC. To achieve this they created a lentiviral vector system that monitors both APOBEC- and ...

...-studied lentivirus is HIV, which many lentiviral tools have been derived from. Lentiviruses have slightly...adenovirus has the highest maximum titer, a plus for production and infection. This virus can infect dividing... with immortalized cell line cultures. Lentivirus Lentivirus is a type of retrovirus and shares many ... commonly used lab viruses: gamma-retrovirus, lentivirus, adenovirus, and adeno-associated virus. Gamma-retrovirus...gamma-retrovirus, coming in around 9 kb. Unlike gamma, lentivirus can infect both dividing and non-dividing cells...nucleus as gamma-retroviruses do. Fun fact: Since lentivirus and gamma-retrovirus are so closely related, ...specific virus? We have you covered with guides on lentivirus, retrovirus, adenovirus, and AAV. If you’re wondering...

...precursors, biofuels, and natural products without the undesirable side products generated during chemical synthesis...these molecules. To make large amounts of pure product in an affordable way, researchers test many different... resistance through gene stacking, increasing production of a protein of interest, and improving the nutrient...Fragmid system was used to assess how different lentiviral plasmid backbone elements impact viral titer ...Modular Assembly Toolkit for the Rapid Design and Production of Diverse Expression Vectors.” ACS Synthetic...expand the Fragmid toolkit to facilitate other lentivirus- and AAV-based studies. Unified MoClo Systems...