... the antibody has an antigen binding site for a total of 2 binding sites per antibody. C) IgG’s are big...23495938. Additional Resources on the Addgene Blog DIY DNA ladders from Penn State University Use Nanobodies...

...Cas9’s second HNH endonuclease domain. Cpf1 cleaves DNA in a staggered pattern and requires only one RNA ...Kim et al. Kim et al. also compared the ratio of total off-target to on-target modification for AsCpf1 ...

... viability controls such as DNA binding dyes and amine reactive dyes. DNA binding dyes, such as propidium...create a histogram of CD19-containing cells versus total cell number to determine the number of B cells in...membranes as is the case with dead or dying cells. DNA binding dyes are not compatible with protocols that...amine reactive dye, such as LIVE/DEAD stain. Like DNA binding dyes, amine reactive dyes can only pass through...

... biotin ligase from A. aeolicus, which lacks the DNA-binding domain (233 amino acids) found in the E. ...Central PMCID: PMC3699407. Milo, Ron. "What is the total number of protein molecules per cell volume? A call...

...plasmids are transformed in E. coli with the donor DNA for editing to take place. Then plasmids are cured.... coli W strain, and 1 Tatumella citrea, with a total of 70 sites edited. We optimized the strategy for...

...interactions between DNA and protein. Micrococcal nuclease digestion is used to shear the DNA in these assays... uses antibodies to capture protein:DNA complexes. After capture, the DNA is released from the complex...suitable for DNA:histone modifier interactions, which have strong binding between the protein and DNA. Some ...Alternatively, the samples can be normalized to a total protein measurement. Both methods will also serve...light. Shear the samples using sonication to break DNA up into fragments of 300-1000 bps in length. Note...IP section above. Reverse the crosslink, isolate DNA, and perform downstream analysis. Special considerations...gentler but results in non-random fragmentation of the DNA. Controls for capture methods Capture methods should...

...sure you hear it all the time, but Addgene is a totally invaluable resource. I really appreciate the work... plasmid request came on September 8, 2004, for pcDNA-p160MBP, while the first request for a kit came ...

...psi packaging signal. Viral components typically total <1.5kb, which is rarely more than 30% of the transfer... created for a variety of applications including cDNA expression, shRNA-mediated knockdown, Tet and Cre-regulated...

... cells and media are harvested and purified. In total, this process takes 4-7 days, not counting the time...are present in an AAV prep. AAV samples are first DNaseI digested to remove any residual AAV plasmid that... account for the dilution of the sample for the DNAseI digest. Use good sterile technique when handling...

...maintained and often results in a greater yield of DNA. However, if the plasmid contains a Gateway cassette...plasmids (resulting in a four plasmid system in total). In addition, 3rd generation systems do not use...1 Genome Nuclear Import Is Mediated by a Central DNA Flap. Zennou V, Petit C, Guetard D, Nerhbass U, Montagnier... polypurine tract; recognition site for proviral DNA synthesis. Increases transduction efficiency and ... simple transfections. Is it feasible to express cDNA from a lentiviral transfer plasmids normally used...drive RNA pol III-directed transcription of shRNAs. cDNA expression requires RNA pol II, and thus requires...