We narrowed to 144 results for: transfection

...be produced through a single or dual-plasmid transfection, depending on whether the heavy and light chain...they’ve been separated onto two. You’ll first transfect your cells with your plasmid(s), and then wait...

...packaging and host genome integration. After co-transfection of these plasmids, 293T cells released transgene-containing...work with primary cells or other difficult-to-transfect cells. Lentiviral barcoding libraries have been...

...human codon-optimized SpCas9; sgRNA can be co-transfected as PCR amplicons (Ran et al., Nature Protocols... #61422 - #61424 were designed for transient transfection : 61422 : Expresses dCAS9-VP64 activator with...codon-optimized SaCas9; SaCas9 sgRNA can be co-transfected as PCR amplicons using a protocol similar to ...

...identical targeting vectors delivered through transfection of naked DNA. Other investigators who had been...then packaged into AAV virions via transient transfection of 293T cells with helpers, and used to infect...

...generating hiPSCs for clinical purposes: mRNA transfection- this allows transient expression of the reprogramming...express reprogramming factors To date, the mRNA transfection method has not been used successfully to derive...

...site. (c) The pCAG-EGxxFP target plasmid was co-transfected with pX330 plasmids containing sgRNA sequences...efficacy of your gRNAs is then a simple matter of transfecting HEK293T cells with your modified pCAG-EGXXFP...

...the passage number of a culture the lower the transfection and transduction efficiencies. Cultures should... while others opt for a single harvest after transfecting with the transfer and packaging plasmids. A ...

...short interfering RNA). Delivery can occur via transfection or viral delivery of a plasmid. shRNAs are often...

...Addgene's Biosafety Resources Protocols General Transfection Bert Vogelstein’s lab practical guide for using...

...as effective NIR fluorescence in transiently transfected live HeLa cells with no supply of exogenous BV...and after normalization to fluorescence of co-transfected EGFP. e Originally reported as a monomer16, IFP2.0...