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We narrowed to 469 results for: Lif

Showing: 101 - 120 of 469 results

  1. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ...scFv sequences have traditionally been cloned by amplifying the VLand VH antibody sequences of hybridomas...for high affinity scFvs. Bound phage are then proliferated by infecting bacteria for additional rounds ... high affinity binding. Bound phage are then proliferated by infecting bacteria for additional rounds ...applications of scFvs: The SunTag system uses an scFv to amplify the fluorescence intensity of a tagged protein.... about the SunTag system which uses an scFv to amplify the fluorescence intensity of a tagged protein....

  2. Technical Design of a Western Blot

    Type
    Blog Post
    ...multiple sites on an antigen, are cheap and can help amplify a weak signal. However, they have high cross-reactivity... smells like rotting eggs but has a longer shelf life when stored properly. Because BME is not as strong...hours Easy and cheap to handcast Short shelf-life; can alter proteins due to high pH Bis-tris ...proteins <40 kDa 180 V, 30 minutes Good shelf life; runs at neutral pH (reduced protein alteration)...proteins > 30 kDA 200 V, 30 minutes Good shelf life; runs at neutral pH (reduced protein alteration)... that work as signal enhancers. These blockers amplify signal without increasing background noise and ...

  3. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...CyOFP1, a large stokes shift, long fluorescence lifetime, and a stable monomeric form as determined by ...resonance energy transfer) and FLIM (Fluorescence Lifetime Imaging Microscopy) applications. To demonstrate...isolated and characterized: mScarlet (fluorescent lifetime of 3.9 ns, quantum yield of 0.70), mScarlet-I ...maturation in cells compared to mScarlet, fluorescent lifetime of 3.1 ns, quantum yield of 0.54) and mScarlet-H...photostability compared to mScarlet, fluorescent lifetime of 1.3 ns, quantum yield of 0.20). All three mScarlet...comprised of 2 plasmids, pPSU1 and pPSU2, that can be amplified affordably in bacteria. They are compatible with...

  4. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ... whole genome amplification (by a mechanism known as multiple displacement amplification) even in the ...that is refractory to enzymatic manipulation, amplifying phage DNA with phi29 polymerase prior to fragmentation...cloned into E. coli cells, isolated colonies will be lifted onto nitrocellulose membranes and exposed to IPTG...the plate (saving the plate so you can regrow the lifted colonies overnight), flip the membrane over and...

  5. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    ...immunocytochemistry assays. Dewari et al, eLife 2018;7:e35069 DOI: 10.7554/eLife.35069. Encouraged by the high ...Operetta imaging system. Dewari et al, eLife 2018;7:e35069 DOI: 10.7554/eLife.35069. How to set up csRNP knock-in...mammalian stem cells using Cas9 ribonucleoprotein. Elife 7. PubMed PMID: 29638216. PubMed Central PMCID: ...

  6. Who Gives a Tweet? 9 Facts About Scientists on Twitter

    Type
    Blog Post
    ...scientists are overrepresented on Twitter Mathematical, life, and physical scientists are underrepresented. It...domains between 0-10% of the time; however, ~20% of life scientists  (biologists, neuroscientists, ecologists...follower network is shown. Top: The follower network of life (green), physical (blue) and social (orange)  scientists...subgroups of scientific disciplines: social (left), life (center), and physical (right). How do you use Twitter...

  7. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...major research applications. eLife, 12, RP91645. https://doi.org/10.7554/eLife.91645 Biddle, M. S., Alende.../FTD disease gene C9ORF72. eLife, 8, e48363. https://doi.org/10.7554/eLife.48363 Uhlen, M., Bandrowski...of interest is critical to cell survival or proliferation, an additional challenge is that only partial...

  8. Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs

    Type
    Blog Post
    ...growth opportunities in life sciences       Propel Careers: Propel Careers is a life science search and career...Immigration officers use to determine if an applicant qualifies for the National Interest Waiver. The three prongs...and no job offer is needed for this category. To qualify, you need to satisfy 3 out of the 10 criteria listed...assist companies and foreign nationals working in the life sciences community obtain their working visas, permanent...

  9. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...as the spectrum shift occurs during Timer protein life due to a form of oxidation (dehydrogenation). MitoTimers...that you need. The Golden GATEway cloning kit simplifies the cloning process for complex DNA constructs...signaling dynamics are influenced by the relatively long life-cycle of a protein from expression to degradation...the same transgene as the Brainbow cassette, simplifying experiments when additional cross-breeding steps...between the vector and gene primer for SLIC cloning. Amplify the pCoofy vector and the sequence of gene of interest...cassette, which is not copied during the PCR amplification step, so that only vectors with the desired ...

  10. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    ...are having difficulty amplifying your target sequence, or if you want to amplify sequences from a large...efficient, but if you are having difficulties in amplifying a template, particularly from a large genome,...correct insert Sequence the regions that have been amplified by PCR, putting special attention in the areas...

  11. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    ...detected with tyramide signal amplification. Tyramide signal amplification occurs when HRP converts a fluorescently...Tyramide signal amplification of HRP Staining cultured cells and tissue sections Amplifying weak signals...

  12. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    ...and a Senior Research Associate at the Labor and Worklife Program at Harvard Law School. She recently launched...is a Senior Research Associate at the Labor and Worklife Program at Harvard Law School. He has continually...R. "A fair deal for PhD students and postdocs." Elife 2 (2013): e01139. Pubmed PMID: 24137543. Pubmed ...Marder, Eve. "Looking out for future scientists." eLife 3 (2014): e04901. Pubmed PMID: 25291257. Pubmed ...

  13. Meet an Addgenie: Insights into Grad School, Career Paths, and More

    Type
    Blog Post
    ... of my life, even though that’s what I thought,” she said. “I’m so excited to see where my life will take... as a research and technical assistant, when her life changed––she had a daughter. Needing a career switch...she loves to do, she would never work a day in her life. She said, “it now makes sense because I love working...

  14. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    ...for experimental use. Thus, the first step is to amplify your library to a concentration that is sufficient...next-generation sequencing to check the quality of your amplification. If you've obtained a ready-to-use lentiviral.... Figure 2: The CRISPR library must be amplified (A) to be used to generate lentivirus (B). Cas9... As such, sequencing CRISPR libraries after amplification and after a screen requires the use of next-...

  15. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

    Type
    Blog Post
    ...beta platform for sharing, annotating, and storing life science protocols. Since we began sharing this protocol...Currently in beta testing, the software aims to be a life science protocol repository. The software can be...have a record of your work. This feature further simplifies lab work if you like to bring your phone in the...and track inventory and orders. Quartzy caters to life science labs and offers the service free of charge...

  16. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ...Expansion Microscopy (TREx). Elife 11:e73775. https://doi.org/10.7554/elife.73775  Dani A, Huang B, Bergan...Expansion microscopy of C. elegans. Elife 9:e46249. https://doi.org/10.7554/elife.46249  Additional resources ...

  17. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ...inclusive. CBE Life Sciences Education. 13(4):602-606. Brian, D. 1996, Einstein: A Life. Chapter 17, Einstein... gains and positive STEM attitudes in CUREs. CBE Life Sciences Education. 21(4):ar74. Duboue, E., Kowalko...chemical biology, biochemistry, and neurobiology. CBE Life Sciences Education. 15(4):ar55. Ma, Y., Mukherjee...

  18. Plasmids 101: Colony PCR

    Type
    Blog Post
    ...yes or no” kind of test, with a positive clone amplifying a product and a negative clone resulting in no...and without an insert to verify that the primers amplify the expected size PCR products.    Primer Design...same backbone plasmid. If this control doesn’t amplify a product, then you know there could be something...insert-specific primers. If your negative control amplifies a product of the expected size, you know the genome...

  19. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    ...species, even from completely different kingdoms of life. That’s convenient for researchers who want to do...episomal vectors is to include viral oris to drive amplification, which helps increase cellular uptake, but this... DNA intercalator, which affects all kingdoms of life. Of course, that includes you. Besides the risk ... you hop, skip, and jump between all kingdoms of life. A bit of careful design will help make your experiment...

  20. The Challenges of Cell Culture

    Type
    Blog Post
    ...leading to genotypic and phenotypic artefacts. The life science literature is a litany of false interpretation...Down-Regulation of HIF-1α by NDRG2 Expression Attenuates Proliferation and Invasion in Renal Cancer Cells, Marker-independent...attributions (from top to bottom) Database Center for Life Science (DBCLS),  National Cancer Institute, Public... Skloot, Rebecca, and Bahni Turpin. The immortal life of Henrietta Lacks. New York: Crown Publishers, ...
Showing: 101 - 120 of 469 results