We narrowed to 160 results for: ALC

1. Protocol - How to Inoculate a Bacterial Culture

   Type

   Protocol

   ...mini-prep you will usually want to start 2 mL in a falcon tube, but for larger preps you might want to use...

2. Molecular Biology Protocol - Restriction Digest of Plasmid DNA

   Type

   Protocol

   ...reaction in 1 hour. Using this ratio, you can calculate the minimal amount of enzyme for your reaction...

3. Protocol - Bacterial Transformation

   Type

   Protocol

   ...50 μL of competent cells in a microcentrifuge or falcon tube. GENTLY mix by flicking the bottom of the ...

4. Lentivirus Production

   Type

   Protocol

   ... Da Heat-inactivated FBS 1x PBS pH 7.4 without calcium or magnesium, Corning 21-040-CV (cations can affect...

5. Plasmid Cloning by Restriction Enzyme Digest (with Protocols)

   Type

   Protocol

   ...base pairs for each varies, it is difficult to calculate this based on DNA concentration alone. One method...

6. Virus Protocol - Generating Stable Cell Lines

   Type

   Protocol

   ...mL), Millipore TR-1003-G 1X PBS pH 7.4 without calcium or magnesium, Corning 21-040-CV (cations can affect...

7. Western Blot

   Type

   Protocol

   ... 37 °C . Determine the absorbance at 590 nm . Calculate the average absorbance of the duplicate samples...

8. Antibody Validation Using the Indirect ELISA Method

   Type

   Protocol

   ...don’t forget to factor in that dilution when calculating the original sample concentration!). Sample Data...

9. AAV ddPCR Titration

   Type

   Protocol

   ... a CSV file and use the following equation to calculate the titer for each sample: $$T = {R*C*D*1000\over...

10. Protocol - pLKO.1 – TRC Cloning Vector

    Type

    Protocol

    ... transfection reagent in serum-free OPTI-MEM. Calculate the amount of Fugene® and OPTI-MEM necessary given...