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We narrowed to 650 results for: CHI;

Showing: 151 - 180 of 650 results

  1. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

    Type
    Blog Post
    ...Guided by dCas9, VP64 recruits transcriptional machinery to specific sequences, causing targeted gene regulation...levels. Although other methods have been able to achieve much higher activation, dCas9-VP64 is great for...of interest, this allows more transcriptional machinery to be recruited per targeted gene. Performance...experiment! References Bikard D, Jiang W, Samai P, Hochschild A, Zhang F, Marraffini LA (2013) Programmable...Ewen-Campen B, Chari R, Ter-Ovanesyan D, Haque SJ, Cecchi RJ, Kowal EJK, Buchthal J, Housden BE, Perrimon...

  2. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ... A., Ichiki, T., Mazmanian, S. K., Osborne, P. B., Keast, J. R., Miller, C. T., Fox, A. S., Chiu, I. M...the brain difficult. Traditionally, delivery is achieved through the use of invasive methods, limiting ...control of transgene expression or activity can be achieved using switches, including riboswitches and optogenetic...Introduction to AAV References Asencor, A. I., Dvoryanchikov, G., Makhoul, V., Tsoulfas, P., & Chaudhari,... W. R., Fish, K. N., & Pfenning, A. R. (2022). Machine learning sequence prioritization for cell type-...j.ymthe.2019.07.017 Matsuzaki, Y., Konno, A., Mochizuki, R., Shinohara, Y., Nitta, K., Okada, Y., & Hirai...Vormstein-Schneider, D., Lin, J. D., Pelkey, K. A., Chittajallu, R., Guo, B., Arias-Garcia, M. A., Allaway, K...

  3. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

    Type
    Blog Post
    ...smaller pieces, and proximity ligation creates chimeric circles of DNA that are distant linearly but close...“picture” is then developed by detecting these chimeric DNA fragments by PCR or next-generation sequencing... outperformed other 3D chromatin methods such as ChIA-PET or Hi-C, by increasing both the number of unique...on-target enrichment. 2. Determining the spatial and hierarchical organization of chromatin interaction networks...CAPTURE 2.0 is the construction of spatial and hierarchical chromatin interaction networks. Think of these...

  4. Transferable Skills: Negotiation

    Type
    Blog Post
    ...partners, and/or children about everything from what you’re going to eat for dinner (frozen chicken fingers or...issue with childcare timing pickup. Whoever takes the earlier slot agrees to pick up both children while the...that the brand new grad student keeps using the machine? Let’s walk through a more specific example....

  5. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type
    Blog Post
    ...Filter By…” options changed when we changed from searching for viral preps instead of plasmids? The available... use to find materials from this publication. Searching for the title of a paper works You can also find...search results to exactly what you need: Better searching with filters Not every plasmid search will start...in the previous layout, the number of plasmids matching each filter is displayed in parentheses.  In this...insert, or use the other options to find plasmids matching your search terms with gene and guide RNA / shRNA...

  6. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type
    Blog Post
    ...they had even established their own labs.  Fried chicken with a side of inspiration  I look back at the ...randomized coffee trials for us at ASCB|EMBO 2019, matching Mentors with Singletons for short coffee breaks... even more valuable program to Microbe 2020 in Chicago.  Finding our place in networking and mentoring...Conference, and the Society for Advancement of Chicanos/Hispanics and Native Americans in Science’s (SACNAS...Joe taught high school chemistry for a year. The teaching/mentoring bug never went away. While he has frequent...

  7. Hot Plasmids - November 2023

    Type
    Blog Post
    ...problem! We also have chimeric versions of Anti-PSD-95 [K28/43R] in rabbit, chicken, and rat. Find Anti-...Tracking cellular history with protein ticker tape Teaching an old antibody new tricks   New compact, efficient... into the TurboID enzyme, Lee and Cheah et al. achieved precise control over when and where the enzyme... bioRxiv — doi: 10.1101/2021.10.13.463862.    Teaching an old antibody new tricks By Ashley Waldron While...

  8. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type
    Blog Post
    ...ophthalmology & visual science 59.3 (2018): 1630-1640. Sakaguchi, Richi, Marcus N. Leiwe, and Takeshi Imai. "Bright... Zebrabow animals to an inducible Cre line. The Schier group developed Zebrabow as a system that takes...optimizes Brainbow for current technologies Tetbow (Sakaguchi et al., 2018) overcame the low expression of XFP...such as the brain, transparent so that the 3D architecture of a fluorescent neuron is visible. Chemical...

  9. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ...therapeutic settings. Silencing of a target gene is achieved through the delivery of a double-stranded RNA ... plasmids, shRNAs hijack the endogenous miRNA machinery for processing (Figure 1). The shRNA sequence ...the bound miRISC can simply block translation machinery. The composition of the RISC varies, but at the...% end_module_attribute %}{% module_attribute "child_css" is_json="true" %}{% raw %}{}{% endraw %}{% end_module_attribute... Silencing Complex: a versatile gene-silencing machine. Journal of Biological Chemistry, 284(27), 17897...

  10. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    ...has been achieved with the Rinehart system (6); however different proteins will likely achieve different...suppression, as described (6). To determine the stoichiometry of phospho-incorporated to non-phospho-incorporated...-Sung, et al. "Expanding the genetic code of Escherichia coli with phosphoserine." Science 333.6046 (2011...al. "A flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation...

  11. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    ...obvious that a desired career outcome is not easily achieved. Of note, it was highlighted during the conference...Positive Points for Trainees - Taking Steps to Achieve a Successful Career Career Advice from the Career...showing researchers that they can take steps to achieve success in their careers. The take-away message...research. As during previous panels, speakers Moon Duchin, Rafael Luna, Joan Reede, Alberto Roca, and Jessica...and stability of academic research. Panelist Moon Duchin provided a concrete example of how underrepresented...

  12. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ...Mizutani E, Sato H, Kasai M, Ogawa A, Suchy F, Yamaguchi T, Nakauchi H (2018) Intra-embryo Gene Cassette Knockin...mouse embryos, the embryos allowed to develop into chimeric mice (with a fraction of the cells in the adult...adult mice derived from the engineered ESCs), and chimeric adults mated to produce completely transgenic ...Wang is a professor at the Institute of Zoology, Chinese Academy of Sciences, and he is interested in using...

  13. What's New in CRISPR - Winter 2018

    Type
    Blog Post
    ...dual-nuclease Cas9-Cas9 chimeras Scot Wolfe’s lab has developed dual-nuclease Cas9-Cas9 chimeras with higher target... in Nature Communications Find the Cas9-Cas9 chimera plasmids A modular toolkit for genome editing...transcription factor localization and binding via ChiP-seq. Learn more about ENCODE Find the transcription...

  14. Science Communication for Everyone

    Type
    Blog Post
    ... Many thanks to our guest blogger, Chinmaya Sandangi! Dr. Chinmaya Sadangi is currently a postdoc at the...This post was contributed by guest blogger Chinmaya Sadangi, a postdoc at the University of Toronto. ...goal of communicating science to non-scientists. Chinmaya Sadangi, a postdoc at the University of Toronto...

  15. Science Communication Snapshot: DayCon 2015

    Type
    Blog Post
    ...well as archived seminar presentations. One of SITN's newest initiatives, DayCon was the brainchild of SITN...Currently in its 16th year, Science in the News has two chief goals. First, SITN works to bridge the communication...information about SITN: DayCon event website (includes archived content) Science in the News website Check our...

  16. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    ...tumors effectively in mice. Chimeric antigen receptors T cells producing chimeric antigen receptors (CARs)...expressing CD19 chimeric antigen receptors for acute lymphoblastic leukaemia in children and young adults...proteins involved in immune tolerance. Recently, China spearheaded the first ever CRISPR-based clinical...

  17. 2023 and 2024: Reflections and Looking Ahead

    Type
    Blog Post
    ...wonderful opportunity to once again reflect on our achievements in the last year and share the exciting things...distributor partners, with the launch of antibodies in China and expansion of viral services available in South...partially through work with our distributors in China, Korea, Japan, India, and Brazil. Through an expansion...companies, while making use of our nonprofit status to achieve mission-driven goals of accelerating research and...

  18. Plasmids 101: Origin of Replication

    Type
    Blog Post
    ...chromosomal DNA, but they still rely on the host machinery to make additional copies. ori sequences are generally...lower temperatures and allows the replication machinery room to come in and get busy making copies.   ...incompatible because they will compete for the same machinery, creating an unstable and unpredictable environment...multiple-unnatural amino acid mutagenesis in Escherichia coli. Biochemistry. 2013 Mar 12;52(10):1828-37...

  19. Viral Vectors 101: Transductions

    Type
    Blog Post
    ...another method Transfection and transduction seem to achieve similar outcomes, so why and when should you opt...Second, what is the biological outcome you want to achieve? For example, do you want the nucleic acid cargo...work is that you can swap out your virus type to achieve different biological outcomes. For example, some...targeting of that virus, which can’t be easily achieved with alternative methods. Ready, set, transduce...

  20. Don’t Wait To Launch Your Health Science Startup

    Type
    Blog Post
    ....  You will find an extra layer of difficulty launching a company in the health sciences without those...guidance.  Before even beginning the process of launching a company, you need to determine whether or not...viable.  This is not to say that we aren’t still pitching for feedback.  Your venture should continuously...you know your idea, you will rarely succeed in launching a company without partners.  You need a dedicated...

  21. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    ...This post was contributed by Adam Chin-Fatt, a Ph.D. student at the University of Western Ontario. Adam...the overexpression of various metabolites in Escherichia coli or Saccharomyces cerevisiae with few techniques...programs. Thank you to our guest blogger! Adam Chin-Fatt is a Ph.D. student at the University of Western...recombinant pharmaceuticals. Follow him on Twitter @AdamChinFatt     References Qi, L. S. et al. Repurposing...

  22. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    ...Presentation Experience”, “Career Achievements”, and “Academic Achievements”. Leaning my CV more towards me...time in the lab, my colleagues and what I was researching, but I decided I needed a new challenge. Looking... business friendly CV that would focus on my achievements, capabilities, courses and office skills. I ...

  23. Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs

    Type
    Blog Post
    ...but not limited to biotechnology, chemistry, architecture, engineering, mathematics, physical sciences...should only focus on your current scientific achievements. Participating in the scientific review process...international recognition for your outstanding achievements in your specific academic field, (2) that you...receipt of major prizes or awards for outstanding achievement Evidence of membership in associations that require...require their members to demonstrate outstanding achievement Evidence of published material in professional...

  24. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    ... as many viral particles must infect a cell to achieve visible expression of the protein. However, expression...expression of receptors may not need to be as high to achieve an observable signal in your experiment, therefore...looking for effective gene expression. How do you achieve high titers in the first place? Some AAVs are known...solution. Add a bit less buffer than you would to achieve the exact desired concentration to avoid over-diluting...

  25. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    ... need, and we wanted to support all of them — searching, browsing, drilling down, and zooming out — with...that best match a user’s query, and return the matching resources in an order that makes sense to the ...synapsin, hsyn synapsin-1, human synapsin, hSynap. Matching We capture multiple variations of the same information...ID). Depositor suggestions provide shortcuts to matching PI labs and institutions. There are many more ...

  26. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    ...oligos can be cloned to create a chimeric guide RNA. Find pX330-U6-Chimeric_BB-CBh-hSpCas9. pSpCas9(BB)-2A-...years on their own or through Addgene!) pX330-U6-Chimeric_BB-CBh-hSpCas9 - The S. pyogenes Cas9 (SpCas9) has... This plasmid expresses the S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from the U6...

  27. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type
    Blog Post
    ...thanks to our guest bloggers Joachim Goedhard and Marieke Mastop! Joachim Goedhart is an assistant professor...This post was contributed by guest bloggers Joachim Goedhart and Marieke Mastop from the Section of Molecular...fluorescent probes. You can follow him on twitter: @joachimgoedhart.   Marieke Mastop is a PhD candidate at the...

  28. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type
    Blog Post
    ... Arnold’s lab and Viviana Gradinaru’s lab used machine learning to design new channelrhodopsins that are...channelrhodopsin that’s successful in vivo. Using machine-learning to overcome channelrhodopsin design challenges...challenges, the Arnold and Gradinaru Labs developed a machine-learning model to help them construct “designer...Robinson JE, Mackey ED, Gradinaru V, Arnold FH (2019) Machine learning-guided channelrhodopsin engineering enables...

  29. What's New in CRISPR - November 2021

    Type
    Blog Post
    ...recognition by MMR machinery and improved editing efficiency. The lab also optimized the architecture and amino...Enhanced prime editing systems by manipulating MMR machinery In their Cell paper, the Liu Lab found that mismatch... developed a system in which the cellular MMR machinery is disrupted via a dominant negative mutation ...

  30. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type
    Blog Post
    ...thanks to our guest bloggers Joachim Goedhart and Marieke Mastop! Joachim Goedhart is an assistant professor...This post was contributed by guest bloggers Joachim Goedhart and Marieke Mastop from the Section of Molecular...fluorescent probes. You can follow him on twitter: @joachimgoedhart.   Marieke Mastop is a PhD candidate at the...
Showing: 151 - 180 of 650 results