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Showing: 151 - 180 of 379 results
  1. RUBY-Red Siliques

    Type
    Blog Post
    ... plants (from callus) or T1 plants (Arabidopsis floral dip). In the next generation the CRISPR construct...right) A RUBY-expressing Arabidopsis seed following floral dip transformation next to an untransformed seed...
  2. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    ...school could afford – led me to develop BioBits ExplorerTM and BioBits BrightTM, biology kits based on freeze-dried...   References 1. Huang, Ally, et al. "BioBits™ Explorer: A modular synthetic biology education kit." Science...
  3. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    ...companies. The space was modern with clean bold colors of orange and white. The decor was very contemporary...Addgene lab, we don’t often get the opportunity to explore new techniques in our own space. Workshops such...
  4. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    ...thawed the samples at room temperature and used the color of the liquid solution and pH paper to measure the...samples were at the control pH of 7.4 as indicated by color (data not shown). What we did next: Venting the ...
  5. Science Career Options

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    Blog Post
    ...actually want, you can feel lost. Use this post to explore the wide range of careers available to scientists...roles. These types of skills show that you fully explore the different options available and then decide...
  6. A Quick Guide to a Career in Software Product Management

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    Blog Post
    ...others, and have strong communication skills to tailor your message to any type of audience. Additionally...more and more people will have the opportunity to explore a career in product management.  A day in the life...
  7. Antibodies 101: ChIP

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    Blog Post
    ... ChIP is an incredibly useful application for exploring these interactions, and can give valuable insight... with a PCR purification kit or with a phenol-chloroform extraction.    Fig. 1: Schematic overview...
  8. RANbodies: Reporter Nanobody Fusions

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    Blog Post
    ...Chicken IgY domains are especially useful for multi-color staining of tissue because they have limited cross-reactivity...cultured cells and tissue sections Great for multicolor staining of tissue since it won’t cross-react...
  9. Plasmids 101: The protein expression toolbox

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    Blog Post
    ...tried-and-true protein expression control tools as well as explore some new innovations. Whether you want to turn ...introduced gene or over the native locus? Let’s explore each of the options together. Turn it off Turn...
  10. Working with Nuclear Receptors

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    Blog Post
    ...reporter gene has over the years evolved from chloramphenicol acetyltransferase to luciferase, the basic ... Professor of Molecular and Cellular Biology at Baylor College of Medicine and Editor-In-Chief of the ...
  11. Hot Plasmids - October 2020

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    Blog Post
    ...synapses or that are Cre-inducible, researchers can explore synaptic integration and connectivity without the...Guo and her colleagues at Yale University fused a color-changing fluorescent timer (FT) protein to the histone...
  12. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    ...standard method for purification of rAdV uses cesium chloride (CsCl) density gradients combined with ultracentrifugation...practical guide for using the AdEasy System. 3. Jager, Lorenz, et al. "A rapid protocol for construction and ...
  13. CRISPR in the Clinic

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    Blog Post
    ...example, in 2013, Jinsong Li and colleagues chose to explore CRISPR’s therapeutic potential in mice with a dominant...component, CAR. Clinical trials are underway to explore CRISPR’s capacity for both classes of edits in ...
  14. Of Myc and Men

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    Blog Post
    ...ll dive into those questions in this post as we explore… the life and times of the myc tag.  Origins of...that their new monoclonals would help the field explore Myc’s role in cell proliferation and tumorigenesis...
  15. Academic vs. Industry Postdocs

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    Blog Post
    ...quickly. Of course, you might have less time to explore scientifically since you can’t afford to spend ...time working on something extremely risky or exploratory. Getting a job afterwards: Fairly or unfairly...
  16. DNA Purification Without a Kit

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    Blog Post
    ...remove RNA contamination and you can use a phenol chloroform extraction to remove remaining proteins and RNase...concentration of a chaotropic salt (guanidine hydrochloride for the plasmid purification protocol and guanidine...
  17. CRISPR Antimicrobials

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    Blog Post
    ...but also alter an individual’s native, beneficial flora. Currently, there is no antibiotic strategy available...population, represented here by cells of different colors, specifically targets bacteria with the given genomic...
  18. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

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    Blog Post
    ...processes. Re-engineering GFP to increase its range of color and application Almost as soon as its sequence... not listed here, many permutations within each color also exist with only slight variations separating...
  19. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    ... their selection techniques to come up with new colors (Fig. 3), such as Radish and Carrot, and further... ligands. References 1. Ma, Hanhui, et al. “Multicolor CRISPR labeling of chromosomal loci in human ...
Showing: 151 - 180 of 379 results