We narrowed to 379 results for: Lor
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TypeBlog Post...more details on how to make the most of these exploratory conversations. If you set yourself a goal of ...to Find Funding For Your Research University of Florida: Training Grants and Other Funding Opportunities...
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RUBY-Red Siliques
TypeBlog Post... plants (from callus) or T1 plants (Arabidopsis floral dip). In the next generation the CRISPR construct...right) A RUBY-expressing Arabidopsis seed following floral dip transformation next to an untransformed seed... -
Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring
TypeBlog Post...Lab at Stanford University became interested in exploring biological dark matter – large tracts of the microbial... microbial tree of life that remained unexplored. Using new single-cell sequencing approaches, the lab... -
Creating accessible biology activities in schools with BioBits
TypeBlog Post...school could afford – led me to develop BioBits ExplorerTM and BioBits BrightTM, biology kits based on freeze-dried... References 1. Huang, Ally, et al. "BioBits™ Explorer: A modular synthetic biology education kit." Science... -
FPbase: A new community-editable fluorescent protein database
TypeBlog Post...entities with a large range of characteristics (color, brightness, photostability, maturation, oligomerization...Protein Choosing Your Fluorescent Proteins for Multicolor Imaging Resources on Addgene.org Browse all... -
Learning Technical Skills at a Lab Central Workshop
TypeBlog Post...companies. The space was modern with clean bold colors of orange and white. The decor was very contemporary...Addgene lab, we don’t often get the opportunity to explore new techniques in our own space. Workshops such... -
How Dry Ice Affects Viral Vector Sample pH & How to Avoid It
TypeBlog Post...thawed the samples at room temperature and used the color of the liquid solution and pH paper to measure the...samples were at the control pH of 7.4 as indicated by color (data not shown). What we did next: Venting the ... -
Science Career Options
TypeBlog Post...actually want, you can feel lost. Use this post to explore the wide range of careers available to scientists...roles. These types of skills show that you fully explore the different options available and then decide... -
Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish
TypeBlog Post...plant compounds to treat complications of diabetes Gloria Otito Izu, PhD Candidate, University of Ibadan,...Oluwatosin Adeyi, University of Ibadan, Nigeria Gloria Otito Izu is a PhD Candidate working under the ... -
A Quick Guide to a Career in Software Product Management
TypeBlog Post...others, and have strong communication skills to tailor your message to any type of audience. Additionally...more and more people will have the opportunity to explore a career in product management. A day in the life... -
Antibodies 101: ChIP
TypeBlog Post... ChIP is an incredibly useful application for exploring these interactions, and can give valuable insight... with a PCR purification kit or with a phenol-chloroform extraction. Fig. 1: Schematic overview... -
Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection
TypeBlog Post...in the lab. This is commonly done using calcium chloride which permeabilizes the cell membrane so the bacteria...phases of competence occur during the Helicobacter pylori growth cycle." FEMS microbiology letters 255.1 ... -
Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer
TypeBlog Post... in the presentation, starvation or the use of caloric restriction mimetics trigger autophagy and, in ... References: 1. Pietrocola, Federico, et al. "Caloric restriction mimetics enhance anticancer immunosurveillance... -
Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards
TypeBlog Post...tdTomato and assessed cFos expression. She will explore the connectivity patterns of these cells and the...during morphine administration and withdrawal to explore how these cells may encode the negative affect ... -
It’s Bioelectric! An Exciting Interdisciplinary Field of Research
TypeBlog Post...Hyperpolarizing chloride channel SwiChr++ - A channelrhodopsin converted into a chloride-conducting anion... -
RANbodies: Reporter Nanobody Fusions
TypeBlog Post...Chicken IgY domains are especially useful for multi-color staining of tissue because they have limited cross-reactivity...cultured cells and tissue sections Great for multicolor staining of tissue since it won’t cross-react... -
Plasmids 101: The protein expression toolbox
TypeBlog Post...tried-and-true protein expression control tools as well as explore some new innovations. Whether you want to turn ...introduced gene or over the native locus? Let’s explore each of the options together. Turn it off Turn... -
Working with Nuclear Receptors
TypeBlog Post...reporter gene has over the years evolved from chloramphenicol acetyltransferase to luciferase, the basic ... Professor of Molecular and Cellular Biology at Baylor College of Medicine and Editor-In-Chief of the ... -
Hot Plasmids - October 2020
TypeBlog Post...synapses or that are Cre-inducible, researchers can explore synaptic integration and connectivity without the...Guo and her colleagues at Yale University fused a color-changing fluorescent timer (FT) protein to the histone... -
Adenoviral Vector Production and Troubleshooting
TypeBlog Post...standard method for purification of rAdV uses cesium chloride (CsCl) density gradients combined with ultracentrifugation...practical guide for using the AdEasy System. 3. Jager, Lorenz, et al. "A rapid protocol for construction and ... -
CRISPR in the Clinic
TypeBlog Post...example, in 2013, Jinsong Li and colleagues chose to explore CRISPR’s therapeutic potential in mice with a dominant...component, CAR. Clinical trials are underway to explore CRISPR’s capacity for both classes of edits in ... -
Of Myc and Men
TypeBlog Post...ll dive into those questions in this post as we explore… the life and times of the myc tag. Origins of...that their new monoclonals would help the field explore Myc’s role in cell proliferation and tumorigenesis... -
Academic vs. Industry Postdocs
TypeBlog Post...quickly. Of course, you might have less time to explore scientifically since you can’t afford to spend ...time working on something extremely risky or exploratory. Getting a job afterwards: Fairly or unfairly... -
DNA Purification Without a Kit
TypeBlog Post...remove RNA contamination and you can use a phenol chloroform extraction to remove remaining proteins and RNase...concentration of a chaotropic salt (guanidine hydrochloride for the plasmid purification protocol and guanidine... -
CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics
TypeBlog Post...techniques to explore dark matter in the genome. There are countless unknown regions yet to explore. I hope ... -
CRISPR Antimicrobials
TypeBlog Post...but also alter an individual’s native, beneficial flora. Currently, there is no antibiotic strategy available...population, represented here by cells of different colors, specifically targets bacteria with the given genomic... -
Fluorescent Proteins 101: Green Fluorescent Protein (GFP)
TypeBlog Post...processes. Re-engineering GFP to increase its range of color and application Almost as soon as its sequence... not listed here, many permutations within each color also exist with only slight variations separating... -
What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program
TypeBlog Post...’ll choose to study Crepidula atrasolea or Prochlorococcus with your next project. Gymnotiformes and Mormyridae...evolution in immunology, neurobiology, and more. Prochlorococcus (Penny Chisholm) This cyanobacterium is the... -
Analyzing CRISPR Editing Results with ICE from Synthego
TypeBlog Post... she's either day-dreaming about exploring sci-fi worlds or exploring nature in the real world. Additional... -
Fluorescent Proteins 101: Aptamer Fluorophores
TypeBlog Post... their selection techniques to come up with new colors (Fig. 3), such as Radish and Carrot, and further... ligands. References 1. Ma, Hanhui, et al. “Multicolor CRISPR labeling of chromosomal loci in human ...