... produce all of the differentiated cells in an organism, including the embryonic and extraembryonic cell...quantities in specific “stem cell niches” found in many organs in the adult human body. These generate specialized...PMCID: PMC3347549. Additional Resources on the Addgene Blog pCXLE Toolkit Stem Cell Models for Disease...Differentiate the Stem Cell Field Resources on Addgene.org Visit Our Stem Cell Page Find Plasmids for Reprogramming...

... 2. Determining the spatial and hierarchical organization of chromatin interaction networks  A second ...techniques: the powerful tools for spatial genome organization deciphering. Molecular Cytogenetics, 11. https...s13059-020-01973-w Additional Resources on the Addgene Blog First time CRISPR user? Check out our tips... Here Brush Up on Your CRISPR Background with Addgene’s CRISPR Guide Page ... Favorite CREs Here Additional Resources on Addgene.org Find Other Cas9 Variants for Purifying Genomic...

...their suppliers, and public health and medical organizations are working to help educate the public about...chance of an opportunistic infection like for organisms like C. difficile. In 2014, Citorik et al. and...PMC4˚237163. Find plasmids from this paper at Addgene. 2. Bikard, David, et al. “Exploiting CRISPR-Cas...PubMed: PMID 26973885. Additional Resources on the Addgene Blog CRISPR Methods for Bacterial Genome Engineering... out of the Genome  Additional Resources on Addgene.org Find CRISPR Plasmids for Your Research Read ...

...the membranes into stacked structures including organized smooth endoplasmic reticulum (OSER) whorls. 2)...distorted into dense stacked membranes termed Organized Smooth Endoplasmic Reticulum or OSER if a dimerizing...can be monomerized with an A206K mutation [2]. Addgene has many of these constructs and they are designated...PMCID: PMC3324619. Additional Resources on the Addgene Blog Read Professor Snapp's Post on mOX FPs Find...in 720°, i.e. they are on a membrane or in an organelle, then their effective concentration will be much...in Your Experiments Additional Resources on Addgene.org Browse our Fluorescent Protein Resources Find...

...become a positive force for public health. Visit Addgene's Microbiology Pages What is antibiotic resistance... that the drug prevents the growth of a living organism. As for multi-drug resistance: definitions vary...vary in the literature, but generally organisms with multi-drug resistance are resistant to three or more...Resistance Fact Sheet  Additional Resources on the Addgene Blog Learn How Antibiotic Resistance Genes Are...is an intense amount of competition between microorganisms for available resources. This competition drives...Our Microbiology Pages Additional Resouces on Addgene.org Check Out Our Microbiology Pages ...

...plant tissue cultures.   C. roseus is not a model organism such as Arabidopsis or tobacco. Studying gene ...transgenic plants is a common problem in non-model organisms. Therefore, transient methods are usually the ...Nicola Patron, who shared the MoClo plant kit via Addgene. For us, transient leaf transformations are a great...PMCID: PMC2699436. Additional resources on the Addgene blog Read all of our plant biology blog posts ...Read our Plasmids 101 blog posts Resources on Addgene.org Browse our Plant Plasmids and Resources page...

...default backgrounds all year and want a change? Addgene has you covered. Today we’re releasing 11 bold ...reading this blog, chances are you are a fan of Addgene or our very cute mascots!  Blugene has been helping...might want to use one of our virus backgrounds. Addgene’s viral service provides ready-to-use AAV and lentiviral...the joke? If not, you might want to check out Addgene’s Fluorescent Proteins blog topic to learn more ...background.     You can find all of the plasmids that Addgene offers by visiting our collections page. Here, ..., you can browse plasmids by field, organism, research technique, and more. Download the green DNA background...background.     Since we ❤️ DNA at Addgene, we're presenting you with another DNA background option, Download...

...shipping look like at Addgene? Here's an inside peak into the daily workings in Addgene's lab. We ship individual... all drive Addgene to succeed and to improve, so thank you. 500,000x Thank You! Addgene's new location...March 26, 2015. How does Addgene make it happen? Our awesome team of Addgenies! Our team recently grew ...Yesterday we hit a huge milestone here at Addgene – 500,000 plasmids shipped! That's a 1/2 million plasmids...labeling stabs with barcodes as they go. Stabs are organized by shipment and moved to our packing area.Using...with shipping labels and paperwork. Our stacks of Addgene boxes are now ready to be sent around the world... to necessitate an office move. We've got ~50 Addgenies working to ship plasmids, analyze QC sequence,...

...the United States but around the world. As an organization that directly collaborates with NIH-funded researchers...utilizes grants to support our own initiatives, Addgene is deeply concerned about these developments. These... We Are Here to Help Despite these challenges, Addgene remains steadfast in our mission to accelerate ...vital during this period of constrained funding. Addgene is uniquely positioned to help research institutions...Universities and research institutions can turn to Addgene as a trusted partner for archiving their biological...scientific progress depends on our collective response. Addgene stands ready to collaborate with partners across...work and what support would be most valuable from Addgene during this time. Your input will directly shape...

...quietly learning about Addgene’s past and present, but the real promise of Addgene lies in our future. As...every aspect of our organization. One thing that will remain the same is Addgene’s commitment to supporting...Addgene saw big changes in 2022, from the launch of a new material to the redesign of a community resource...number of people. I look forward to working with Addgene colleagues, collaborators, partners, and the larger...

...generated using random genomic fragments from the organism of interest, or a defined cDNA library (e.g. the...number of two-hybrid systems available in model organisms, including bacteria, alternative fungi (C. albicans... Pubmed Central PMCID: PMC514463. Resources At Addgene Browse Our Yeast Expression Vectors Find Bacterial...

...specimens and for in vivo imaging of transparent organisms. Advances in tissue clearing Similar to light ...3DISCO (3-dimensional imaging of solvent cleared organs) method of imaging rodent brains and spinal cords...).  These viral plasmids are available through Addgene.  The combination of LSFM with multiple tissue ...

...your host organism. Because transcription machinery differs between cell types or organisms, promoters...writing of this article. Additional Resources on the Addgene Blog Browse All Plasmids 101 Posts Learn about...GFP Resources on Addgene.org Find Plasmids for Your Research     ...  Kendall Morgan...

...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...discussion, we bring up a performance Ben and Niroshi organized for Tyler's (the host's) thesis defense. This ...Additional Resources on the Addgene Blog Get Advice on Choosing a Good Mentor Learn How the Future of Research...Process  Browse our Career Posts Resources on Addgene.org Browse Wu Lab Plasmids Find Plasmids for Your...

...region (approximately 500bp is best) and select the organism. It will then tell you the available gRNA targets...can also find many previously validated gRNAs at Addgene. In vitro screening for the best gRNA Figure...Find Plasmids for Testing Your gRNAs in vitro at Addgene CRISPR/Cas9 gene editing in mice Now that you’ve... much more from our favourite mammalian model organisms in the near future.  Many thanks to our guest...PMCID: PMC3969854. Additional Resources on the Addgene Blog Get the Basics of Mouse Genome Engineering...Homology Directed Repair Additional Resources on Addgene.org Find Software Tools to Help Design You gRNA ...

...for Scientists Resources on Addgene.org Learn about Careers at Addgene ...accordingly. Read Additional Career Posts on the Addgene Blog How did this change in labor laws come about... salaries (which have been made by multiple organizations across academia). Increased postdoc wages, overtime... salaries as their website implies. These 5 organizations, according to the NSF data, account for 18% ...Research @FORsymp.   Additional Resources on the Addgene Blog Read about the 2015 and 2016 Future of Research...

...over-express coding sequences from a given cell type or organism, have been used in a similar manner to Cas9 activators...lines (HEK293T, MCF7, U2-OS, Hela, N2A, 3T3) and organisms (Chavez et al., 2016). To learn more about the...    Alejandro (Alex) Chavez is a member of the Addgene Scientific Advisory Board. His laboratory at Columbia...doi.org/10.1038/nbt.3149 Additional Resources on the Addgene Blog Learn how to Choose the Best Cas9 Variant... CRISPR Has Been Used with AAV Resources on Addgene.org Find Cas9 Activators Find Cas9 Repressors Catch...

...and stably maintain the genes they carry among organisms belonging to many different phylogenetic groups...bacteria and express proteins within different host organisms. pBBR1-based plasmids and plasmids in incompatibility...  Find broad host range recombinant vectors at Addgene!   References and resources References Doran, ...10.1007/s13205-023-03507-0  More resources on the Addgene blog Plasmids 101: Origin of Replication Plasmids...

...Blotting Resources on Addgene.org Find plasmids encoding recombinant antibodies at Addgene ...intact cell, tissue, or, in some cases, a whole organism. But, as with any antibody-based technique, validation...you are confident, especially if you work in an organism that is underrepresented in the biomedical research...10.1038/nmeth.3995 Additional Resources on the Addgene blog Learn about research applications of antibodies...

...plasmids with Addgene. Additional control plasmids for the TKO library will be available at Addgene soon. Hart...cellular compartments (cytosol, Addgene catalog #71245; mitochondria, Addgene catalog #71247) Rosella provides... in vitro cell systems and diverse model organisms. Addgene’s newest TALEN kit, the FusX assembly system...read? Listen to our hot plasmids segment on the Addgene Podcast. View Plasmids from:  March (Q1) | June...sequencing. The STARR-seq backbone is available from Addgene in both human and fly versions. Arnold, et al....alongside a growing number of CRISPR libraries from Addgene’s repository that can be used to perform screens...PMID: 24395366 Oxford Drosophila CRISPR library Addgene has had the privilege of distributing several human...

...  Additional resources on the Addgene blog Get started with Addgene's science career resources Browse...hub assembled a coalition of higher education organizations committed to advancing career and professional...

...plasmids you use to bring this into your target organism. We have designed a Golden Gate system based on...The complete GreenGate toolkit, now available at Addgene, also gives the option to stack several expression...motivations for making GreenGate available through Addgene,” Lohmann said. “We strongly believe the free sharing...  Kendall Morgan...

...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...Along these lines, Darrell recently joined the Addgene Board of Directors where he hopes to help keep ...of this technique will someday allow us to make organs using iPS cells derived from patients. Chapter ...Chapter 12: Darrell's goals as a member of the Addgene board of directors - increasing open source sharing...Additional Resources from Addgene Learn about Retinal Gene Therapy from Connie Cepko Find Plasmids from the...

...protocols Sep-OTSλ plasmid - Addgene # 68292 C321.ΔA bacterial strain – Addgene #68306 pCRT7 NT TOPO E17TAG...E17TAG GFP plasmid - Addgene #68295 SupD plasmid – Addgene # 68307 For further technical reference and protocols...amongst a subset of methanogenic archea: these organisms lacked a classic cysteinyl tRNA synthetase, despite...flavors’ of Sep-OTS plasmids are available through Addgene, but we have worked most extensively worked with... recombinant phosphoproteins’ available on the Addgene website. Many thanks to guest blogger Natalie ... Lajoie, Marc J., et al. "Genomically recoded organisms expand biological functions." Science 342.6156...Protoc. doi 10 (2008). Journal. Resources on the Addgene Blog Verify Your Constructs with Colony PCR Read...

...should come as no surprise that those of us at Addgene believe in the power of sharing, and especially...ban gene patents in a case against Myriad, an organization called Free the Data launched a public database...And, last but not least (certainly not for us), Addgene launched this blog, A Better Way to Share Science...  Kendall Morgan...

...plasmids available from Addgene Capsid Published Tropism Model Organism Mouse Strain-Specific...administration, distributing widely throughout the organism to target large or diffuse biological structures...good option for your experiment? Look it up in Addgene’s Data Hub and the CLOVER repository of published...specific application and animal model.  Other model organisms Tropism and efficacy can vary depending on species...target cell type or tissue of interest, and model organism under study. *Many engineered AAVs for mice use...most commonly requested systemic capsid in the Addgene repository. Like its parent AAV-PHP-B, it can be... and shows decreased transduction in off-target organs (Goertsen, Flytzanis, et al., 2022). AAV-PHP.N ...

...Brainbow plasmids available from Addgene! Find Brainbow Plasmids @Addgene: Brainbow 1.0, 1.1, 2.1 plasmids...addition to the neuronal pThy1-Brainbow constructs, Addgene also has two Brainbow adeno-associated viral vectors...techniques are also being applied to study model organisms such as Drosophila and zebrafish. Brainbow in ...important insights into the complicated physical organization of the brain and other biological systems. Interested...

...these principles apply to using CRISPR in other organisms. First, consider the genetic manipulation that...carry out your experiment in your particular model organism. There may not be a perfect plasmid for your specific...disrupt gene function in a particular cell type or organism without a specific preferred mutation Cas9 (or...effects Additional Resources: CRISPR protocols from Addgene depositors JOVE Video Reprint: Protocol for genomic...sequence and design your gRNA. Know your cell line/organism and genomic sequence When possible, you should...may vary depending on the specific cell line or organism, which may affect the observed efficiency of CRISPR...containing validated gRNAs are now available through Addgene. These plasmids contain gRNAs that have been used...

...executive director of Addgene, it is my privilege to uphold the values that shape Addgene’s mission and work...mirroring our own aspirations for Addgene. On behalf of every Addgenie, thank you for twenty years of incredible...thrilled to be sharing our plans for 2024.  In 2023, Addgene distributed 188,729 materials to 39,844 scientists... plasmids, deposited from 1,106 labs in 504 organizations from 37 different countries, into our repository... three–year project that touched every team at Addgene. As a resource, it will allow scientists around...Institute for Protein Innovation (IPI), in which Addgene will distribute recombinant antibodies (rAbs) developed...focus on expanding international access to the Addgene repository, partially through work with our distributors...

...our hot plasmids segment on the Addgene Podcast. Subscribe to the Addgene blog to get all the latest hot...transposon vectors tested in this work are available at Addgene. Kim, et al. Stem Cell Reports. 2015. PubMed PMID... using a viral approach Genetically modified organisms like transgenic mice are important tools for understanding...specific to interneurons in mice as well as other organisms, opening the possibility of using these tools ... a stable monomeric form as determined by the organized smooth endoplasmic reticulum (OSER) assay. A major...spines. The set of CyRFP constructs available at Addgene includes bacterial and mammalian mCyRFP1 or CyRFP1...lab has deposited all mScarlet constructs with Addgene. Bindels DS, et al. Nat. Methods. 2017. PubMed...

... a few of the new CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR...zebrafish genome, this system can be used in these organisms. Read the paper in bioRxiv Find the GeneWeld...a new CRISPR tool you’ve recently deposited to Addgene and you’d like it to be included in the next What...more information. Additional resources on the Addgene blog Download the CRISPR 101 eBook Find our CRISPR...posts Read our CRISPR cheat sheet Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids by...

...the observation of proteins in living cells and organisms. Both components of the chimera are encoded by...). However, these assays are qualitative. The Organized Smooth Endoplasmic Reticulum (OSER) assay (Costantini...PMCID: PMC4723041. Additional resources on the Addgene blog Read our fluorescent proteins blog posts ...Read our plasmids 101 blog series Resources on Addgene.org Browse the fluorescent protein collection Find...

...post was contributed by guest bloggers and IMP organizers Rosario F. Godino, Rocío López-Diego, & Zafira... Students also need more access to reputable, organized networks connecting international experts and ...Advancemnet of Higher Education website Resources at Addgene Read Other Career Blog Posts Read the Networking...

... resources on addgene.org  Addgene's CRISPR Guide Addgene's CRISPR 101 eBook  Addgene's CRISPR Cheat Sheet...distinguishing the foreign sequences and keeping the array organized. Target strand: The strand of foreign DNA that...looser PAM requirements, which are available from Addgene!   RNA crRNA: “CRISPR RNAs” are transcribed from...CRISPR-Cas9 system for laboratory applications. At Addgene, we sometimes refer to the programmable guide sequence...Conclusion Ready for some more details? Check out the Addgene CRISPR guide or our CRISPR 101 ebook. We also ... Sheet  Resources on the Addgene blog A Needle in a Base-Stack: Cas9 Structural Biology...

...Additional resources on Addgene.org Find molecular biology protocols here Visit the Addgene careers page ... of different phages or with phages targeting organisms closely related to the clinical sample. For treatment... Capsid and Tail. Additional resources on the Addgene blog Read our blog posts on open science Find ...

...transcriptional machinery from a particular organism or group of organisms. Meaning, if a plasmid in intended ...videos for specific techniques and tips! Addgene Protocols Addgene Video Library...Addgene's molecular biology reference guide, including information on molecular genetics, plasmids, sequencing...abbreviated to A, T, C, and G respectively) that are organized into a double stranded helix. The order of these...the instructions to make every protein within an organism. Proteins are made up of amino acids. Each amino... plasmid. For commonly used primers check out Addgene's sequencing primer list. Working with Plasmids ...can drive gene expression in a wide variety of organisms, including plants, worms, mice, and even cultured...

...power of community in 2024 In 2024, your trust in Addgene helped unlock unprecedented scientific collaboration.... Even more remarkably, 1,160 labs from 522 organizations in 41 countries enriched our repository with...online and in-person, you helped us understand how Addgene can better serve the global scientific community...about our latest developments? Subscribe to the Addgene newsletter. References Ayoubi, R., Ryan, J., Gonzalez...

...manipulation Resources on Addgene.org Browse all viral vectors available at Addgene Visit our viral vectors...tools for optogenetics and chemogenetics on the Addgene website. Optogenetics also requires that light ...brain. One way to address this is by working with organisms with light accessible brains, such as zebrafish...bs.mie.2018.01.022 Additional resources on the Addgene blog Read more about optogenetics New to viral...

... a few of the new CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR.... Caulobacter crescentus is a popular model organism for studying the bacterial cell cycle, asymmetric...a new CRISPR tool you’ve recently deposited to Addgene and you’d like it to be included in the next What...more information. Additional resources on the Addgene blog Download the CRISPR 101 eBook Find our CRISPR...posts Read our CRISPR cheat sheet Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids by...

...for a review, see Simpson et al., 2015). Visit Addgene's MIcrobiology Pages The problem: Host binding proteins...membrane on an agar plate selective for your target organism and incubate overnight according to appropriate...on hand that will specifically bind your target organism, it can be helpful to spot this protein directly...PubMed Central PMCID: PMC2258639. Resources on the Addgene Blog Learn How CRISPR Can Be Combined with Phage...CRISPR For Genome-Wide Screens Resources on Addgene.org Visit Our Microbiology Pages Use the FX Cloning...